| + |
PKA | down-regulates activity 
phosphorylation
|
GRK7 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276036 |
Ser23 |
YLQARKPsDCDSKEL |
in vitro |
|
| pmid |
sentence |
| 15946941 |
We also determined that cAMP-dependent protein kinase (PKA) phosphorylates GRK1 at Ser(21) and GRK7 at Ser(23) and Ser(36) in vitro. These sites are also phosphorylated when FLAG-tagged GRK1 and GRK7 are expressed in HEK-293 cells treated with forskolin to stimulate the endogenous production of cAMP and activation of PKA.Phosphorylation of GRK1 and GRK7 by PKA reduces the ability of GRK1 and GRK7 to phosphorylate rhodopsin in vitro. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-274081 |
Ser23 |
YLQARKPsDCDSKEL |
in vitro |
|
| pmid |
sentence |
| 15946941 |
PKA Phosphorylates GRK7 on Ser23 and Ser36. Phosphorylation by PKA inhibits GRK7 activity |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260839 |
Ser23 |
YLQARKPsDCDSKEL |
Homo sapiens |
|
| pmid |
sentence |
| 15946941 |
Phosphorylation of GRK1 and GRK7 by cAMP-dependent Protein Kinase Attenuates Their Enzymatic Activities | We also determined that cAMP-dependent protein kinase (PKA) phosphorylates GRK1 at Ser(21) and GRK7 at Ser(23) and Ser(36) in vitro. These sites are also phosphorylated when FLAG-tagged GRK1 and GRK7 are expressed in HEK-293 cells treated with forskolin to stimulate the endogenous production of cAMP and activation of PKA. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260840 |
Ser36 |
ELQRRRRsLALPGLQ |
Homo sapiens |
|
| pmid |
sentence |
| 15946941 |
Phosphorylation of GRK1 and GRK7 by cAMP-dependent Protein Kinase Attenuates Their Enzymatic Activities | We also determined that cAMP-dependent protein kinase (PKA) phosphorylates GRK1 at Ser(21) and GRK7 at Ser(23) and Ser(36) in vitro. These sites are also phosphorylated when FLAG-tagged GRK1 and GRK7 are expressed in HEK-293 cells treated with forskolin to stimulate the endogenous production of cAMP and activation of PKA. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-274080 |
Ser36 |
ELQRRRRsLALPGLQ |
in vitro |
|
| pmid |
sentence |
| 15946941 |
PKA Phosphorylates GRK7 on Ser23 and Ser36. Phosphorylation by PKA inhibits GRK7 activity |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276035 |
Ser36 |
ELQRRRRsLALPGLQ |
in vitro |
|
| pmid |
sentence |
| 15946941 |
We also determined that cAMP-dependent protein kinase (PKA) phosphorylates GRK1 at Ser(21) and GRK7 at Ser(23) and Ser(36) in vitro. These sites are also phosphorylated when FLAG-tagged GRK1 and GRK7 are expressed in HEK-293 cells treated with forskolin to stimulate the endogenous production of cAMP and activation of PKA.Phosphorylation of GRK1 and GRK7 by PKA reduces the ability of GRK1 and GRK7 to phosphorylate rhodopsin in vitro. |
|
| Publications: |
6 |
Organism: |
In Vitro, Homo Sapiens |
| + |
PKA | up-regulates activity 
phosphorylation
|
GRK7 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-263152 |
Ser36 |
ELQRRRRsLALPGLQ |
Xenopus laevis |
|
| pmid |
sentence |
| 18803695 |
Recently, we have defined sites of phosphorylation by cAMP-dependent protein kinase (PKA) in the amino termini of both GRK1 and GRK7 in vitro. To determine the conditions under which GRK7 is phosphorylated in vivo, we have generated an antibody that recognizes GRK7 phosphorylated on Ser36, the PKA phosphorylation site. Using this phospho-specific antibody, we have shown that GRK7 is phosphorylated in vivo and is located in the cone inner and outer segments of mammalian, amphibian and fish retinas. The conservation of phosphorylation at Ser36 in GRK7 in these different species (which span a 400 million-year evolutionary period), and its light-dependent regulation, indicates that phosphorylation plays an important role in the function of GRK7 |
|
| Publications: |
1 |
Organism: |
Xenopus Laevis |
| Tissue: |
Retina |
| + |
GRK7 |
phosphorylation
|
GRK7 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260838 |
Ser490 |
YAKDIAEiDDFSEVR |
Homo sapiens |
|
| pmid |
sentence |
| 15946941 |
In the absence of PKA, GRK1 and GRK7 are autophosphorylated | The mutants, S490A-GRK7 and S490E-GRK7, do not undergo autophosphorylation, indicating that Ser490 is the only autophosphorylation site in GRK7 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
GRK7
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