| + |
GRIPAP1 | up-regulates activity 
binding
|
MAP3K1 |
0.316 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260607 |
|
|
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 17761173 |
We investigated signal transduction pathways that might lie downstream of GRASP-1 and found that GRASP-1 potently activates JNK pathway signaling, with no effect on ERK signaling. Such JNK pathway activating activity requires binding of GRASP-1 to both JNK and the upstream JNK pathway activator MEKK-1. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260640 |
|
|
Homo sapiens |
HEK-293T Cell, Brain |
| pmid |
sentence |
| 17761173 |
To examine whether GRASP‐1 interacts with MEKK1 and JNK1 in neurons, co‐immunoprecipitation experiments were performed with detergent‐solubilized extracts from cultured cortical neurons. Both antiJNK1 and anti‐MEKK1 antibodies immunoprecipitated GRASP‐1 from neuronal lysates. These results suggest that GRASP‐1 interacts with MEKK1 and JNK1 in neurons. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
GRIP1 | up-regulates activity
binding
|
GRIPAP1 |
0.529 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260638 |
|
|
Rattus norvegicus |
Brain |
| pmid |
sentence |
| 10896157 |
We have identified several GRIP-associated proteins (GRASPs) that bind to distinct PDZ domains within GRIP. GRASP-1 is a neuronal rasGEF associated with GRIP and AMPA receptors in vivo. GRIP1 has seven PDZ domains, which mediate protein–protein interactions, allowing the recruitment of GRASP-1 to a large signal-transducing complex |
|
| Publications: |
1 |
Organism: |
Rattus Norvegicus |
| + |
GRIPAP1 | up-regulates activity
binding
|
MAPK8 |
0.315 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260639 |
|
|
Homo sapiens |
HEK-293T Cell, Brain |
| pmid |
sentence |
| 17761173 |
To examine whether GRASP‐1 interacts with MEKK1 and JNK1 in neurons, co‐immunoprecipitation experiments were performed with detergent‐solubilized extracts from cultured cortical neurons. Both antiJNK1 and anti‐MEKK1 antibodies immunoprecipitated GRASP‐1 from neuronal lysates. These results suggest that GRASP‐1 interacts with MEKK1 and JNK1 in neurons. GRASP-1 potently activates JNK pathway signaling, with no effect on ERK signaling. Such JNK pathway activating activity requires binding of GRASP-1 to both JNK and the upstream JNK pathway activator MEKK-1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CASP3 | up-regulates activity
cleavage
|
GRIPAP1 |
0.426 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260641 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17761173 |
These results suggest that the region of GRASP‐1 downstream of the Caspase‐3‐cleavage site is capable of activating the JNK signaling pathway by enhancing the phosphorylation of JNK. these results suggest that full length GRASP‐1 does not enhance JNK pathway activity, possibly due to the inhibitory effect of the N‐terminal fragment on the C‐terminal fragment. In contrast, Caspase‐3 cleavage of GRASP‐1 releases the C‐terminal fragment, which in turn activates JNK signaling by serving as a scaffold protein. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
GRIPAP1
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