| + |
hsa-miR-200a-3p | down-regulates quantity by destabilization 
post transcriptional regulation
|
ZEB2 |
0.4 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-281191 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 18376396 |
On the basis of the preceding observations, we surmised that a function of miR-205 and the miR-200 family is to prevent expression of ZEB1 and SIP1 in epithelial cells, which may otherwise trigger EMT by downregulating E-cadherin, and further, that downregulation of the microRNAs may be sufficient to initiate EMT. To test this, we examined the effect of the microRNA inhibitors on cell phenotype.We found that all five members of the microRNA-200 family (miR-200a, miR-200b, miR-200c, miR-141 and miR-429) and miR-205 were markedly downregulated in cells that had undergone EMT in response to transforming growth factor (TGF)-β or to ectopic expression of the protein tyrosine phosphatase Pez.Together, these microRNAs cooperatively regulate expression of the E-cadherin transcriptional repressors ZEB1 (also known as δEF1) and SIP1 (also known as ZEB2), factors previously implicated in EMT and tumour metastasis. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
hsa-miR-200a-3p | down-regulates quantity by destabilization
post transcriptional regulation
|
ZEB1 |
0.4 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-281192 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 18376396 |
On the basis of the preceding observations, we surmised that a function of miR-205 and the miR-200 family is to prevent expression of ZEB1 and SIP1 in epithelial cells, which may otherwise trigger EMT by downregulating E-cadherin, and further, that downregulation of the microRNAs may be sufficient to initiate EMT. To test this, we examined the effect of the microRNA inhibitors on cell phenotype.We found that all five members of the microRNA-200 family (miR-200a, miR-200b, miR-200c, miR-141 and miR-429) and miR-205 were markedly downregulated in cells that had undergone EMT in response to transforming growth factor (TGF)-β or to ectopic expression of the protein tyrosine phosphatase Pez.Together, these microRNAs cooperatively regulate expression of the E-cadherin transcriptional repressors ZEB1 (also known as δEF1) and SIP1 (also known as ZEB2), factors previously implicated in EMT and tumour metastasis. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ZEB1 | down-regulates quantity by destabilization
post transcriptional regulation
|
hsa-miR-200a-3p |
0.4 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-281213 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 24627491 |
Epithelial-mesenchymal transition (EMT) is required for the specification of tissues during embryonic development and is recapitulated during the metastatic progression of tumors. The miR-200 family plays a critical role in enforcing the epithelial state with their expression lost in cells undergoing EMT. EMT can be mediated by activation of the ZEB1 and ZEB2 (ZEB) transcription factors, which repress miR-200 expression via a self-reinforcing double negative feedback loop to promote the mesenchymal state. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TWIST1 | down-regulates quantity by destabilization
post transcriptional regulation
|
hsa-miR-200a-3p |
0.4 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-281209 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20473948 |
The miR-200 family (miR-200a, -200b, -200c, -141 and -429) and miR-205 are frequently silenced in advanced cancer and have been implicated in epithelial to mesenchymal transition (EMT) and tumor invasion by targeting the transcriptional repressors of E-cadherin, ZEB1 and ZEB2. ZEB1 is also known to repress miR-200c-141 transcription in a negative feedback loop, but otherwise little is known about the transcriptional regulation of the miR-200 family and miR-205. TWIST1 associates directly with the miR-200 and miR-205 promoters, and may act as a repressor of miR-200 and miR-205 expression. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ZEB2 | down-regulates quantity by destabilization
post transcriptional regulation
|
hsa-miR-200a-3p |
0.4 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-281216 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 24627491 |
Epithelial-mesenchymal transition (EMT) is required for the specification of tissues during embryonic development and is recapitulated during the metastatic progression of tumors. The miR-200 family plays a critical role in enforcing the epithelial state with their expression lost in cells undergoing EMT. EMT can be mediated by activation of the ZEB1 and ZEB2 (ZEB) transcription factors, which repress miR-200 expression via a self-reinforcing double negative feedback loop to promote the mesenchymal state. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
4.0
hsa-miR-200a-3p
