| + |
NEDD4 | down-regulates quantity 
ubiquitination
|
SAG |
0.375 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278610 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 25216516 |
These data collectively indicate that NEDD4-1 negatively regulates the SAG protein levels.|Third, NEDD4-1 promotes SAG poly-ubiquitylation and degradation, as demonstrated by both in vitro and in vivo ubiquitylation assays. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SAG | up-regulates quantity by stabilization 
binding
|
CUL5 |
0.409 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272844 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 25216516 |
Here we report that NEDD4-1, a HECT domain-containing E3 ubiquitin ligase, binds via its HECT domain directly with SAG's C-terminal RING domain and ubiquitylates SAG for proteasome-mediated. We also found that SAG bridges NEDD4-1 via its C-terminus and CUL-5 via its N-terminus to form a NEDD4-1/SAG/CUL-5 tri-complex. Biologically, NEDD4-1 overexpression sensitizes cancer cells to etoposide-induced apoptosis by reducing SAG levels through targeted degradation. Thus, SAG is added to a growing list of NEDD4-1 substrates and mediates its biological function. degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NEDD4 | down-regulates quantity by destabilization
polyubiquitination
|
SAG |
0.375 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272843 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 25216516 |
Here we report that NEDD4-1, a HECT domain-containing E3 ubiquitin ligase, binds via its HECT domain directly with SAG's C-terminal RING domain and ubiquitylates SAG for proteasome-mediated. We also found that SAG bridges NEDD4-1 via its C-terminus and CUL-5 via its N-terminus to form a NEDD4-1/SAG/CUL-5 tri-complex. Biologically, NEDD4-1 overexpression sensitizes cancer cells to etoposide-induced apoptosis by reducing SAG levels through targeted degradation. Thus, SAG is added to a growing list of NEDD4-1 substrates and mediates its biological function. degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
4.0
SAG
- 
- 