| + |
PLK4 | up-regulates activity 
phosphorylation
|
NEDD1 |
0.591 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272996 |
Ser325 |
PTTVNKRsVNVNAAS |
|
|
| pmid |
sentence |
| 33351100 |
We found that PLK4-mediated phosphorylation of NEDD1 at its S325 amino acid residue directly promotes both NEDD1 binding to SAS-6 and recruiting SAS-6 to the centrosome. |Collectively, our results demonstrate that PLK4-regulated NEDD1 facilitates initiation of the cartwheel assembly and of daughter centriole biogenesis in mammals. |
|
| Publications: |
1 |
| + |
NEK9 | up-regulates activity
phosphorylation
|
NEDD1 |
0.43 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-263016 |
Ser377 |
EKAGLPRsINTDTLS |
in vitro |
|
| pmid |
sentence |
| 22818914 |
Nek9 phosphorylates NEDD1 on Ser377 driving its recruitment and thereby that of γ-tubulin to the centrosome in mitotic cells. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
PLK1 | up-regulates activity
phosphorylation
|
NEDD1 |
0.618 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272994 |
Ser397 |
GKNQDFSsFDDTGKS |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 19509060 |
Here we report that the function of Nedd1 is regulated by Cdk1 and Plk1. During mitosis, Nedd1 is firstly phosphorylated at T550 by Cdk1, which creates a binding site for the polo-box domain of Plk1. Then, Nedd1 is further phosphorylated by Plk1 at four sites: T382, S397, S637 and S426. The sequential phosphorylation of Nedd1 by Cdk1 and Plk1 promotes its interaction with gamma-tubulin for targeting the gammaTuRC to the centrosome and is important for spindle formation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272992 |
Ser426 |
VNKGSDEsIGKGDGF |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 19509060 |
Here we report that the function of Nedd1 is regulated by Cdk1 and Plk1. During mitosis, Nedd1 is firstly phosphorylated at T550 by Cdk1, which creates a binding site for the polo-box domain of Plk1. Then, Nedd1 is further phosphorylated by Plk1 at four sites: T382, S397, S637 and S426. The sequential phosphorylation of Nedd1 by Cdk1 and Plk1 promotes its interaction with gamma-tubulin for targeting the gammaTuRC to the centrosome and is important for spindle formation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272991 |
Ser637 |
HSLLERYsVNEGLVA |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 19509060 |
Here we report that the function of Nedd1 is regulated by Cdk1 and Plk1. During mitosis, Nedd1 is firstly phosphorylated at T550 by Cdk1, which creates a binding site for the polo-box domain of Plk1. Then, Nedd1 is further phosphorylated by Plk1 at four sites: T382, S397, S637 and S426. The sequential phosphorylation of Nedd1 by Cdk1 and Plk1 promotes its interaction with gamma-tubulin for targeting the gammaTuRC to the centrosome and is important for spindle formation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272993 |
Thr382 |
PRSINTDtLSKETDS |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 19509060 |
Here we report that the function of Nedd1 is regulated by Cdk1 and Plk1. During mitosis, Nedd1 is firstly phosphorylated at T550 by Cdk1, which creates a binding site for the polo-box domain of Plk1. Then, Nedd1 is further phosphorylated by Plk1 at four sites: T382, S397, S637 and S426. The sequential phosphorylation of Nedd1 by Cdk1 and Plk1 promotes its interaction with gamma-tubulin for targeting the gammaTuRC to the centrosome and is important for spindle formation. |
|
| Publications: |
4 |
Organism: |
Homo Sapiens |
| + |
AURKA | up-regulates activity
phosphorylation
|
NEDD1 |
0.555 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272965 |
Ser405 |
FDDTGKSsLGDMFSP |
Homo sapiens |
U2-OS Cell |
| pmid |
sentence |
| 31028180 |
Microtubule nucleation during central spindle assembly requires NEDD1 phosphorylation on serine 405 by Aurora A| In the absence of Aurora A, the HURP (also known as DLGAP5) and NEDD1 proteins that are involved in nucleation of microtubules fail to concentrate in the midzone. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CDK1 | up-regulates activity
phosphorylation
|
NEDD1 |
0.583 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272972 |
Ser460 |
STSVLHSsPLNVFMG |
|
|
| pmid |
sentence |
| 21690413 |
Mechanistically, we demonstrated that Cdc2-dependent phosphorylation on a γ-tubulin ring complex (γ-TuRC) recruitment protein, Nedd1/GCP-WD, at the previously uncharacterized S460 residue induces the Nedd1-Plk1 interaction |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272973 |
Thr550 |
PPINGSStPNPKIAS |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 19509060 |
Here we report that the function of Nedd1 is regulated by Cdk1 and Plk1. During mitosis, Nedd1 is firstly phosphorylated at T550 by Cdk1, which creates a binding site for the polo-box domain of Plk1. Then, Nedd1 is further phosphorylated by Plk1 at four sites: T382, S397, S637 and S426. The sequential phosphorylation of Nedd1 by Cdk1 and Plk1 promotes its interaction with gamma-tubulin for targeting the gammaTuRC to the centrosome and is important for spindle formation. |
|
| Publications: |
2 |
Organism: |
, Homo Sapiens |
| + |
HAUS complex | up-regulates activity
relocalization
|
NEDD1 |
0.543 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262332 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19369198 |
We further found coprecipitation of HA-tagged NEDD1 [also called GCP-WD, a component of gamma-TuRC (12, 22)] with endogenous hDgt6|A link between augmin and gamma-TuRC is likely critical for these functions, because a gamma-TuRC mutant that attenuates interaction with augmin does not restore function in vivo |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262331 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19427217 |
Under NuMA and HAUS6 codepletion conditions, NEDD1 levels on spindle microtubules remained low, suggesting that the suppression observed is not caused by more efficient localization of NEDD1 to spindle microtubules (Figure S9D). Taken together, our results suggest that HAUS and NuMA exert opposing activities necessary for robust bipolar spindle formation. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
NEDD1 | up-regulates activity
binding
|
g-TuRC complex |
0.766 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-261422 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19029337 |
It has been reported that NEDD1 directly interacts with and recruits the gamma-tubulin ring complex to centrosomes and to spindle MTs to promote MT nucleation and spindle assembly |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
HAUS6 | up-regulates activity
binding
|
NEDD1 |
0.813 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-261421 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 19029337 |
FAM29A recruits NEDD1 to spindle MTs. Ectopically expressed NEDD1 and FAM29A interact with each other. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
NEDD1
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