| + |
BTRC | down-regulates quantity by destabilization 
ubiquitination
|
GLI3 |
0.653 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-145116 |
Lys773 |
RRNPAGTkWMEHVKL |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 17283082 |
Third, we and others have recently shown that only phosphorylated Ci/Gli3 are able to directly bind Slimb/BetaTrCP, that Gli3 is polyubiquitinated in the cell, and that mutations of 4 lysine residues, the putative ubiquitination sites in the Gli3 C-terminal region, inhibit Gli3 processing These observations further support the notion that Ci/Gli3 processing is carried out by the proteasome because the deletion of the cleavage site is expected to often disrupt the protease-mediated site-specific cleavage. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-249576 |
Lys779 |
TKWMEHVkLERLKQV |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 17283082 |
Third, we and others have recently shown that only phosphorylated Ci/Gli3 are able to directly bind Slimb/BetaTrCP, that Gli3 is polyubiquitinated in the cell, and that mutations of 4 lysine residues, the putative ubiquitination sites in the Gli3 C-terminal region, inhibit Gli3 processing These observations further support the notion that Ci/Gli3 processing is carried out by the proteasome because the deletion of the cleavage site is expected to often disrupt the protease-mediated site-specific cleavage. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-249577 |
Lys784 |
HVKLERLkQVNGMFP |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 17283082 |
Third, we and others have recently shown that only phosphorylated Ci/Gli3 are able to directly bind Slimb/BetaTrCP, that Gli3 is polyubiquitinated in the cell, and that mutations of 4 lysine residues, the putative ubiquitination sites in the Gli3 C-terminal region, inhibit Gli3 processing These observations further support the notion that Ci/Gli3 processing is carried out by the proteasome because the deletion of the cleavage site is expected to often disrupt the protease-mediated site-specific cleavage. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-249578 |
Lys800 |
LNPILPPkAPAVSPL |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 17283082 |
Third, we and others have recently shown that only phosphorylated Ci/Gli3 are able to directly bind Slimb/BetaTrCP, that Gli3 is polyubiquitinated in the cell, and that mutations of 4 lysine residues, the putative ubiquitination sites in the Gli3 C-terminal region, inhibit Gli3 processing These observations further support the notion that Ci/Gli3 processing is carried out by the proteasome because the deletion of the cleavage site is expected to often disrupt the protease-mediated site-specific cleavage. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-143171 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 16371461 |
Phosphorylated gli3 can bind beta-trcp directly both in vitro and in vivo, resulting in polyubiquitination of gli3 and processing through proteasome activity |
|
| Publications: |
5 |
Organism: |
Homo Sapiens |
| Pathways: | Sonic Hedgehog |
| + |
PRKACA | down-regulates quantity
phosphorylation
|
GLI3 |
0.439 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-75339 |
Ser1006 |
GHGVRRAsDPVRTGS |
Homo sapiens |
|
| pmid |
sentence |
| 10693759 |
Ci/gli zinc finger proteins mediate the transcriptional effects of hedgehog protein signals. In drosophila, ci action as transcriptional repressor or activator is contingent upon hedgehog-regulated, pka-dependent proteolytic processingall six pka phosphorylation sites are required for processing of gli3. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-75343 |
Ser849 |
NMLNRRDsSASTISS |
Homo sapiens |
|
| pmid |
sentence |
| 10693759 |
Ci/gli zinc finger proteins mediate the transcriptional effects of hedgehog protein signals. In drosophila, ci action as transcriptional repressor or activator is contingent upon hedgehog-regulated, pka-dependent proteolytic processingall six pka phosphorylation sites are required for processing of gli3. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-75347 |
Ser865 |
YLSSRRSsGISPCFS |
Homo sapiens |
|
| pmid |
sentence |
| 10693759 |
Ci/gli zinc finger proteins mediate the transcriptional effects of hedgehog protein signals. In drosophila, ci action as transcriptional repressor or activator is contingent upon hedgehog-regulated, pka-dependent proteolytic processingall six pka phosphorylation sites are required for processing of gli3. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-75351 |
Ser877 |
CFSSRRSsEASQAEG |
Homo sapiens |
|
| pmid |
sentence |
| 10693759 |
Ci/gli zinc finger proteins mediate the transcriptional effects of hedgehog protein signals. In drosophila, ci action as transcriptional repressor or activator is contingent upon hedgehog-regulated, pka-dependent proteolytic processingall six pka phosphorylation sites are required for processing of gli3. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-75355 |
Ser907 |
TDASRRSsEASQSDG |
Homo sapiens |
|
| pmid |
sentence |
| 10693759 |
Ci/gli zinc finger proteins mediate the transcriptional effects of hedgehog protein signals. In drosophila, ci action as transcriptional repressor or activator is contingent upon hedgehog-regulated, pka-dependent proteolytic processingall six pka phosphorylation sites are required for processing of gli3. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-75359 |
Ser980 |
VHAPRRCsDGGAHGY |
Homo sapiens |
|
| pmid |
sentence |
| 10693759 |
Ci/gli zinc finger proteins mediate the transcriptional effects of hedgehog protein signals. In drosophila, ci action as transcriptional repressor or activator is contingent upon hedgehog-regulated, pka-dependent proteolytic processingall six pka phosphorylation sites are required for processing of gli3. |
|
| Publications: |
6 |
Organism: |
Homo Sapiens |
| Pathways: | Sonic Hedgehog |
| + |
GLI3 | down-regulates
binding
|
MED12 |
0.523 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-149876 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17000779 |
We propose that activated gli3 physically targets med12 in mediator to reverse mediator-dependent suppression of shh target gene (i.e., Gli1 or cyclin d1) transcription. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
GSK3B | down-regulates quantity by destabilization
phosphorylation
|
GLI3 |
0.513 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-148475 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 16885213 |
Gli2 and Gli3 (in vertebrates) are phosphorylated by protein kinase A and glycogen synthase kinase-3_ and are proteolytically processed |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-219231 |
|
|
Drosophila melanogaster |
|
| pmid |
sentence |
| 11955435 |
We show that these phosphoserines prime further phosphorylation at adjacent Glycogen Synthase Kinase 3 (GSK3) and Casein Kinase I (CK1) sites. Alteration of the GSK3 or CK1 sites prevents Ci-155 proteolysis and activates Ci in the absence of Hedgehog. |
|
| Publications: |
2 |
Organism: |
Mus Musculus, Drosophila Melanogaster |
| Pathways: | Sonic Hedgehog |
| + |
SPOP | down-regulates quantity
ubiquitination
|
GLI3 |
0.689 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-268861 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 20463034 |
RNAi knockdown of Spop (a substrate-binding adaptor for the cullin3-based ubiquitin E3 ligase) in Sufu mutant mouse embryonic fibroblasts (MEFs) can restore the levels of Gli2 and Gli3 full-length proteins |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ULK3 | up-regulates activity 
phosphorylation
|
GLI3 |
0.542 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260799 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 19878745 |
We show that ULK3 is able to phosphorylate three mammalian GLI proteins in vitro |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
GLI3 | up-regulates quantity by expression 
transcriptional regulation
|
CCND1 |
0.576 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-188878 |
|
|
Homo sapiens |
Breast Cancer Cell, Lung Cancer Cell |
| pmid |
sentence |
| 19860666 |
Gli activators bind to gaccaccca motif to regulate transcription of gli1, ptch1, ptch2, hhip1, mycn, ccnd1, ccnd2, bcl2, cflar, foxf1, foxl1, prdm1 (blimp1), jag2, grem1, and follistatin. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-154234 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17419683 |
Gli activators bind to gaccaccca motif to regulate transcription of gli1, ptch1, ptch2, hhip1, mycn, ccnd1, ccnd2, bcl2, cflar, foxf1, foxl1, prdm1 (blimp1), jag2, grem1, and follistatin. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
GLI3 | down-regulates quantity by repression 
transcriptional regulation
|
MYCN |
0.369 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-188881 |
|
|
Homo sapiens |
Breast Cancer Cell, Lung Cancer Cell |
| pmid |
sentence |
| 19860666 |
Gli activators bind to gaccaccca motif to regulate transcription of gli1, ptch1, ptch2, hhip1, mycn, ccnd1, ccnd2, bcl2, cflar, foxf1, foxl1, prdm1 (blimp1), jag2, grem1, and follistatin. .Hedgehog Signals induce cellular proliferation through upregulation of n-myc, cyclin d/e, and foxm1. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-154237 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17419683 |
Gli activators bind to gaccaccca motif to regulate transcription of gli1, ptch1, ptch2, hhip1, mycn, ccnd1, ccnd2, bcl2, cflar, foxf1, foxl1, prdm1 (blimp1), jag2, grem1, and follistatin. .Hedgehog Signals induce cellular proliferation through upregulation of n-myc, cyclin d/e, and foxm1. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
ZIC1 | up-regulates
relocalization
|
GLI3 |
0.365 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-105497 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 11238441 |
Co-expression of zic1 resulted in gli1 and gli3 proteins being translocated to the nucleus in varying levels |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RAB23 | down-regulates
|
GLI3 |
0.562 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-143160 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 16364285 |
Based on su(fu) function, we predict that rab23 can interact with all gli1 molecules including gli1, gli2 and gli3, and inhibit their transcriptional activities and nuclear localization. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SUFU | down-regulates activity
relocalization
|
GLI3 |
0.885 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129068 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 10433919 |
Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| Pathways: | Sonic Hedgehog |
| + |
GLI3 | up-regulates quantity by expression
transcriptional regulation
|
PTCH1 |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-188884 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19860666 |
GLI activators bind to GACCACCCA motif to regulate transcription of GLI1, PTCH1, PTCH2, HHIP1, MYCN, CCND1, CCND2, BCL2, CFLAR, FOXF1, FOXL1, PRDM1 (BLIMP1), JAG2, GREM1, and Follistatin |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Sonic Hedgehog |
| + |
ZIC1 | up-regulates
|
GLI3 |
0.365 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-105500 |
|
|
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 11238441 |
Moreover, gli proteins were translocated to cell nuclei by coexpressed zic proteins, and both proteins regulated each others transcriptional activity.In Nih3t3 and 293t cells, both gli1 and gli3 proteins were located predominantly in the cytoplasm (fig. 2, c, d, h, k, l, and p). Coexpression of zic1 resulted in gli1 and gli3 proteins being translocated to the nucleus in varying levels (fig. 2, e and m). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PRKACA | down-regulates
phosphorylation
|
GLI3 |
0.439 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-154276 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17419683 |
In vertebrates,pka-mediated phosphorylation of gli2 and gli3 initiates a phosphorylation cascade that leads to processing into repressors of transcription or frank degradation |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-75362 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 10693759 |
In vertebrates,pka-mediated phosphorylation of gli2 and gli3 initiates a phosphorylation cascade that leads to processing into repressors of transcription or frank degradation |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| Pathways: | Sonic Hedgehog |
| + |
GLI3 | up-regulates quantity by expression
transcriptional regulation
|
PTCH1 |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-209641 |
|
|
Mus musculus |
MEF Cell |
| pmid |
sentence |
| 16571352 |
Primary mouse embryonic fibroblasts responded to Shh stimulation with the induction of Hh target genes Gli1, Ptc1, and Hip1.These observations support the previously advanced notion of a functional redundancy or cooperativity between Gli2 and Gli1 in activation of target genes [18] and [43] and indicate a functional cooperation between Gli3 and Gli1. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-154240 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17419683 |
Binding of n-shh to ptch1 inhibits repression of smo, leading to activationof some genes and de-repression of others through the effects of smo on the gli family of transcription factors. |
|
| Publications: |
2 |
Organism: |
Mus Musculus, Homo Sapiens |
| Pathways: | Sonic Hedgehog |
| + |
SUFU | up-regulates quantity by stabilization
binding
|
GLI3 |
0.885 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-268868 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 20463034 |
We show that loss of suppressor of fused (Sufu; an inhibitory effector for Gli proteins) results in destabilization of Gli2 and Gli3 full-length activators but not of their C-terminally processed repressors, whereas overexpression of Sufu stabilizes them. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Sonic Hedgehog |
| + |
CSNK1A1L | up-regulates
phosphorylation
|
GLI3 |
0.339 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-144554 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 16481469 |
Ci is phosphorylated by pka at multiple sites priming phosphorylation by both gsk3 and cki, leading to partial proteolysis. The pka, gsk3, and cki sites are conserved in gli2 and gli3, vertebrate homologs of ci that are similarly processed |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ZIC3 | up-regulates
binding
|
GLI3 |
0.381 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-157637 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17764085 |
Zic3 functions as a transcriptional coactivator of gli3 when it physically associates with gli3 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
KIF7 | up-regulates quantity by stabilization
binding
|
GLI3 |
0.559 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-209614 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 19592253 |
These results suggest a role for Kif7 in coordinating Hh signal transduction at the tip of cilia and preventing Gli3 cleavage into a repressor form in the presence of Hh. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| Pathways: | Sonic Hedgehog |
| + |
GLI3 | down-regulates quantity
transcriptional regulation
|
FGF8 |
0.465 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-268949 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 12435361 |
Whereas Fgf8 expression was almost absent in Shh-/- mutants, it was up-regulated in Gli3-/-;Shh-/- double mutants, suggesting that SHH is not required for Fgf8 induction, and that GLI3 normally represses Fgf8 independently of SHH |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
CXCL1 | up-regulates quantity by expression
transcriptional regulation
|
GLI3 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-148460 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 16885213 |
The data suggest that smo is in fact the source of two signals relevant to the activation of gli: one involving g(i) and the other involving events at smo's c-tail independent of g(i). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CSNK1A1 | down-regulates
phosphorylation
|
GLI3 |
0.575 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-116512 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 11955435 |
In principle, pka, ck-1 and gsk3 can phosphorylate as many as 19 serine residues in gli3: fourpkasites, three primarygsk3sites, four primary ck-1 sites and eight secondary gsk3 and ck-1 sites |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
GLI3 | down-regulates quantity by repression
transcriptional regulation
|
GLI1 |
0.433 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-209638 |
|
|
Mus musculus |
MEF Cell |
| pmid |
sentence |
| 16571352 |
The basal expression of Gli1, Ptc1, and Hip1 was positively associated with the loss of Gli3 alleles.These findings implicate Gli3 as a repressor of Hh target gene expression. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| Pathways: | Sonic Hedgehog |
| + |
SUFU | down-regulates
relocalization
|
GLI3 |
0.885 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-72308 |
|
|
Homo sapiens |
Prostate Gland Cancer Cell, Melanoma Cell, Glioblastoma Cell |
| pmid |
sentence |
| 10564661 |
Su(fu) is a negative regulator of shh that interacts with all three gli proteins to retain them in the cytosol. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Sonic Hedgehog |
3.0
GLI3
- 
- 