| + |
ARAF | up-regulates 
phosphorylation
|
MAP2K1 |
0.734 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-236451 |
Ser218 |
VSGQLIDsMANSFVG |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 8621729 |
Our data demonstrated that a-raf is, indeed, a mek1 activator and may play a role in growth factor signaling|The immunoprecipitates were assayed for GST-MEK1 activation. D, activation of MEK1 by A-Raf requires the presence of serine residue 218 and 222. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-235944 |
Ser222 |
LIDSMANsFVGTRSY |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 8621729 |
Our data demonstrated that a-raf is, indeed, a mek1 activator and may play a role in growth factor signaling|The immunoprecipitates were assayed for GST-MEK1 activation. D, activation of MEK1 by A-Raf requires the presence of serine residue 218 and 222. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
PAK1 | up-regulates
phosphorylation
|
ARAF |
0.266 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-236342 |
Ser299 |
KNLGYRDsGYYWEVP |
Homo sapiens |
Melanoma Cell |
| pmid |
sentence |
| 15710605 |
Phosphorylation of endogenous a-raf, b-raf and raf-1 on the homologous pak phosphorylation sites (serine 299, serine 445, or serine 338 respectively)we found that the phosphorylation of a-raf on serine 299 was also stimulated by egf, although the duration of phosphorylation on this site was much shorter than for raf-1. Thus, by analogy with raf-1, phosphorylation of this site may play an important role in the a-raf activation mechanism. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | EGFR Signaling |
| + |
ARAF | up-regulates activity
phosphorylation
|
SLC9A3R2 |
0.379 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-273503 |
Ser303 |
QESGLHLsPTAAEAK |
Homo sapiens |
JEG-3 Cell |
| pmid |
sentence |
| 26310448 |
We also identify A-Raf as a kinase necessary for E3KARP phosphorylation at the G2/M stage of the cell cycle. Phosphorylation of Ser-303 regulates the localization, function, and dynamics of E3KARP |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SRC | up-regulates
phosphorylation
|
ARAF |
0.487 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-236037 |
Tyr301 |
LGYRDSGyYWEVPPS |
Chlorocebus aethiops |
COS Cell |
| pmid |
sentence |
| 9020159 |
A-raf behaves like raf-1, being weakly activated by oncogenic ras more strongly activated by oncogenic src, and these signals synergize to give maximal activation. Activation of Raf-1 and A-Raf by Src requires tyrosine phosphorylation at residues 340 and 341 in Raf-1 and 301 and 302 in A-Raf. |
|
| Publications: |
1 |
Organism: |
Chlorocebus Aethiops |
| Pathways: | EGFR Signaling |
| + |
SRC | up-regulates activity
phosphorylation
|
ARAF |
0.487 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-236459 |
Tyr302 |
GYRDSGYyWEVPPSE |
Chlorocebus aethiops |
|
| pmid |
sentence |
| 9020159 |
A-raf behaves like raf-1, being weakly activated by oncogenic ras more strongly activated by oncogenic src, and these signals synergize to give maximal activation |
|
| Publications: |
1 |
Organism: |
Chlorocebus Aethiops |
| Pathways: | EGFR Signaling |
| + |
HRAS | up-regulates
binding
|
ARAF |
0.837 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-175183 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21779497 |
The raf family of proteins (raf-1, a-raf, and b-raf) is serine/threonine kinases that bind to the effector region of ras-gtp, thus inducing translocation of the protein to the plasma membrane. Once there, raf proteins are activated and phosphorylated by different protein kinases. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | EGFR Signaling |
| + |
ARAF | up-regulates
phosphorylation
|
MAP2K2 |
0.748 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-175142 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21779497 |
Active raf phosphorylates mek. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
KSR1 | up-regulates activity
binding
|
ARAF |
0.564 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-273876 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 29433126 |
In mammals, RAF family kinases include three catalytically competent enzymes (ARAF, BRAF and CRAF) and two pseudokinases (KSR1 and KSR2) that have been described as scaffolds owing to their apparent ability to bridge RAF isoforms and their substrate, mitogen-activated protein kinase kinase (MEK).Kinase suppressor of Ras (KSR) pseudokinases were also shown to dimerize with kinase-competent RAFs to stimulate catalysis allosterically. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
2-chloro-5-(2-phenyl-5-pyridin-4-yl-1H-imidazol-4-yl)phenol | down-regulates 
chemical inhibition
|
ARAF |
0.8 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-100355 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 12970777 |
At drug concentrations around the reported ic(50) for the raf inhibitor l-779,450, it suppressed dna synthesis and induced apoptosis in hematopoietic fdc-p1 cells transformed to grow in response to either raf-1 or a-raf (fd/deltaraf-1:er and fd/deltaa-raf:er) |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NRAS | up-regulates
binding
|
ARAF |
0.833 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-175216 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21779497 |
The raf family of proteins (raf-1, a-raf, and b-raf) is serine/threonine kinases that bind to the effector region of ras-gtp, thus inducing translocation of the protein to the plasma membrane. Once there, raf proteins are activated and phosphorylated by different protein kinases. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARAF | down-regulates quantity by destabilization
phosphorylation
|
SMAD2 |
0.374 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279391 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23591895 |
Araf promotes Smad2 linker phosphorylation through S253.|In this study, we demonstrate that Araf can directly bind to and phosphorylate the linker of Smad2, leading to degradation of activated Smad2. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
KSR2 | up-regulates activity
binding
|
ARAF |
0.55 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-273875 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 29433126 |
In mammals, RAF family kinases include three catalytically competent enzymes (ARAF, BRAF and CRAF) and two pseudokinases (KSR1 and KSR2) that have been described as scaffolds owing to their apparent ability to bridge RAF isoforms and their substrate, mitogen-activated protein kinase kinase (MEK).Kinase suppressor of Ras (KSR) pseudokinases were also shown to dimerize with kinase-competent RAFs to stimulate catalysis allosterically. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARAF | up-regulates
phosphorylation
|
MEK1/2 |
0.748 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-244813 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 8621729 |
Our data demonstrated that a-raf is, indeed, a mek1 activator and may play a role in growth factor signaling. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-244809 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21779497 |
Active raf phosphorylates mek. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| Pathways: | EGFR Signaling |
4.0
ARAF
- 
- 