| + |
CDK1 | up-regulates activity 
phosphorylation
|
RCC1 |
0.522 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262702 |
Ser11 |
KRIAKRRsPPADAIP |
in vitro |
|
| pmid |
sentence |
| 15014043 |
Human RCC1 is phosphorylated on Ser 2 and Ser 11 in mitosis by Cdc2 kinase. We show here that Cdc2 kinase phosphorylates the serines located in or near the nuclear localization signal (NLS) of human RCC1, the nucleotide exchange factor for Ran. This phosphorylation is necessary for RCC1 to generate RanGTP on mitotic chromosomes in mammalian cells, which in turn is required for spindle assembly and chromosome segregation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262701 |
Ser2 |
sPKRIAKR |
in vitro |
|
| pmid |
sentence |
| 15014043 |
Human RCC1 is phosphorylated on Ser 2 and Ser 11 in mitosis by Cdc2 kinase. We show here that Cdc2 kinase phosphorylates the serines located in or near the nuclear localization signal (NLS) of human RCC1, the nucleotide exchange factor for Ran. This phosphorylation is necessary for RCC1 to generate RanGTP on mitotic chromosomes in mammalian cells, which in turn is required for spindle assembly and chromosome segregation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262703 |
Ser387 |
GQDEDAWsPVEMMGK |
in vitro |
|
| pmid |
sentence |
| 15014043 |
We show here that Cdc2 kinase phosphorylates the serines located in or near the nuclear localization signal (NLS) of human RCC1, the nucleotide exchange factor for Ran. This phosphorylation is necessary for RCC1 to generate RanGTP on mitotic chromosomes in mammalian cells, which in turn is required for spindle assembly and chromosome segregation. However, when both S2 and S11 were simultaneously mutated to As, the resulting 6His-RCC1S2,11A failed to be phosphorylated, whereas all of the other double mutants were phosphorylated (Fig. 1C). As expected, mutating all four sites to As (the 6His-RCC1S2,11,387A-T274A) also blocked phosphorylation (Fig. 1C). |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262704 |
Thr274 |
SNYHQLGtPGTESCF |
in vitro |
|
| pmid |
sentence |
| 15014043 |
We show here that Cdc2 kinase phosphorylates the serines located in or near the nuclear localization signal (NLS) of hum an RCC1, the nucleotide exchange factor for Ran. This phosphorylation is necessary for RCC1 to generate RanGTP on mitotic chromosomes in mammalian cells, which in turn is required for spindle assembly and chromosome segregation. However, when both S2 and S11 were simultaneously mutated to As, the resulting 6His-RCC1S2,11A failed to be phosphorylated, whereas all of the other double mutants were phosphorylated (Fig. 1C). As expected, mutating all four sites to As (the 6His-RCC1S2,11,387A-T274A) also blocked phosphorylation (Fig. 1C). |
|
| Publications: |
4 |
Organism: |
In Vitro |
| + |
STK3 | up-regulates
phosphorylation
|
RCC1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-263145 |
Ser11 |
KRIAKRRsPPADAIP |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 19559616 |
MST2 Phosphorylates RCC1 In Vitro and In Vivo. Using an antibody generated against phospho-S2/11 in RCC1 [18], we found that these two residues were also efficiently phosphorylated by MST1 and MST2 (Figure 2D), further supporting that S2 and/or S11 are genuine MST2 phosphorylation targets. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-263146 |
Ser2 |
sPKRIAKR |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 19559616 |
MST2 Phosphorylates RCC1 In Vitro and In Vivo. Using an antibody generated against phospho-S2/11 in RCC1 [18], we found that these two residues were also efficiently phosphorylated by MST1 and MST2 (Figure 2D), further supporting that S2 and/or S11 are genuine MST2 phosphorylation targets. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
3.0
RCC1
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