| + |
CSNK2A1 | down-regulates quantity by destabilization 
phosphorylation
|
WEE1 |
0.419 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276038 |
Ser121 |
WEEEGFGsSSPVKSP |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 16085715 |
In the present study, we show that phosphorylation of S123 (pS123) by CDK promoted the binding of Wee1A to beta-TrCP through three independent mechanisms. S123 phosphorylation creates a PBD-binding motif and accelerates S53 phosphorylation by Plk1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PLK1 | down-regulates
phosphorylation
|
WEE1 |
0.623 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-139473 |
Ser123 |
EEGFGSSsPVKSPAA |
Homo sapiens |
|
| pmid |
sentence |
| 16085715 |
Phosphorylation of serines 53 and 123 (s53 and s123) of wee1a by polo-like kinase 1 (plk1) and cdk, respectively, are required for binding to beta-trcp. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-128643 |
Ser53 |
GHSTGEDsAFQEPDS |
Homo sapiens |
|
| pmid |
sentence |
| 15350223 |
Phosphorylation of serines 53 and 123 (s53 and s123) of wee1a by polo-like kinase 1 (plk1) and cdk, respectively, are required for binding to beta-trcp. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-139477 |
Ser53 |
GHSTGEDsAFQEPDS |
Homo sapiens |
|
| pmid |
sentence |
| 16085715 |
Phosphorylation of serines 53 and 123 (s53 and s123) of wee1a by polo-like kinase 1 (plk1) and cdk, respectively, are required for binding to beta-trcp. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-123832 |
Ser53 |
GHSTGEDsAFQEPDS |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 15070733 |
Phosphorylation of serines 53 and 123 (s53 and s123) of wee1a by polo-like kinase 1 (plk1) and cdk, respectively, are required for binding to beta-trcp. |
|
| Publications: |
4 |
Organism: |
Homo Sapiens |
| + |
CDC14A | up-regulates quantity by stabilization 
dephosphorylation
|
WEE1 |
0.568 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-267469 |
Ser123 |
EEGFGSSsPVKSPAA |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 23051732 |
In particular, we found that Cdc14A inhibits Wee1 degradation through the dephosphorylation of Ser-123 and Ser-139 residues. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-267470 |
Ser139 |
YFLGSSFsPVRCGGP |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 23051732 |
In particular, we found that Cdc14A inhibits Wee1 degradation through the dephosphorylation of Ser-123 and Ser-139 residues. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
CDK2 | down-regulates quantity by destabilization
|
WEE1 |
0.647 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276039 |
Ser123 |
EEGFGSSsPVKSPAA |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 16085715 |
PS123 primes CK2 to phosphorylate S121, resulting in creation of a β-TrCP phosphodegron (EEGFGpS121) that is responsible for the instability of Wee1A during interphase. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CDK1 | down-regulates
phosphorylation
|
WEE1 |
0.855 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-123824 |
Ser123 |
EEGFGSSsPVKSPAA |
Homo sapiens |
|
| pmid |
sentence |
| 15070733 |
We have found also that the major M-phase kinases polo-like kinase 1 (Plk1) and Cdc2 are responsible for the phosphorylation of S53 and S123, respectively, and that in each case phosphorylation generates an unconventional phospho-degron (signal for degradation) that can be recognized by beta-TrCP |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-139465 |
Ser123 |
EEGFGSSsPVKSPAA |
Homo sapiens |
|
| pmid |
sentence |
| 16085715 |
We show that phosphorylation of S123 (pS123) by CDK promoted the binding of Wee1A to beta-TrCP through three independent mechanisms. The pS123 not only directly interacted with basic residues in the WD40 repeat domain of beta-TrCP but also primed phosphorylation by two independent protein kinases, Plk1 and CK2 (formerly casein kinase 2) |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
CyclinB/CDK1 | down-regulates quantity by destabilization
phosphorylation
|
WEE1 |
0.778 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-267471 |
Ser123 |
EEGFGSSsPVKSPAA |
|
|
| pmid |
sentence |
| 23051732 |
Cdk1 phosphorylates Wee1 on Ser-123, which primes additional phosphorylation by other kinases, leading to the formation of phosphodegrons responsible for SCF (Skp1/cullin/F-box) ubiquitin-mediated degradation of Wee1 |
|
| Publications: |
1 |
| + |
CDK2 | down-regulates
phosphorylation
|
WEE1 |
0.647 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-139469 |
Ser123 |
EEGFGSSsPVKSPAA |
Homo sapiens |
|
| pmid |
sentence |
| 16085715 |
Phosphorylation of serines 53 and 123 (s53 and s123) of wee1a by polo-like kinase 1 (plk1) and cdk, respectively, are required for binding to beta-trcp. During the s and g2 phases, s123 (wee1) is phosphorylated by a cdk (possibly cdk2). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PLK1 | down-regulates quantity by destabilization
phosphorylation
|
WEE1 |
0.623 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276040 |
Ser53 |
GHSTGEDsAFQEPDS |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 16085715 |
In the present study, we show that phosphorylation of S123 (pS123) by CDK promoted the binding of Wee1A to beta-TrCP through three independent mechanisms. S123 phosphorylation creates a PBD-binding motif and accelerates S53 phosphorylation by Plk1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
BRSK1 |
phosphorylation
|
WEE1 |
0.451 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250601 |
Ser642 |
KKMNRSVsLTIY |
in vitro |
|
| pmid |
sentence |
| 15150265 |
HsSAD1 protein produced in Sf9 cells phosphorylated the GST-fused human wild-type Wee1A fragment but not its S642A mutant produced inEscherichia coli (Fig. 2). The kinase-dead hsSAD1 mutant (K59A) failed to phosphorylate the wild-type Wee1A fragment. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
WEE1 | down-regulates
phosphorylation
|
CDK2 |
0.647 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-83139 |
Tyr15 |
EKIGEGTyGVVYKAR |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 11029659 |
Identification and characterization of human wee1b, a new member of the wee1 family of cdk-inhibitory kinases. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
WEE1 | down-regulates
phosphorylation
|
CDK1 |
0.855 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-139491 |
Tyr15 |
EKIGEGTyGVVYKGR |
Homo sapiens |
|
| pmid |
sentence |
| 16096060 |
The wee1 kinase phosphorylates and inhibits cyclin-dependent kinase 1 (cdk1), thereby delaying entry into mitosis until appropriate conditions have been met |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PAK1 | down-regulates
phosphorylation
|
WEE1 |
0.303 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-123528 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 15037762 |
Kinases targeted sequentially to the neck, cla4/pak and cdc5/polo, are responsible for stepwise phosphorylation and down-regulation of swe1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
1-[6-(2-hydroxypropan-2-yl)-2-pyridinyl]-6-[4-(4-methyl-1-piperazinyl)anilino]-2-prop-2-enyl-3-pyrazolo[3,4-d]pyrimidinone | down-regulates
chemical inhibition
|
WEE1 |
0.8 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-163173 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20068082 |
Mk-1775 (merck). This wee1 inhibitor (ic50, 5.2nm) potentiates the activity of dna-damaging agents (e.g., gemcitabine, cisplatin, carboplatin) in vitro and in vivo, particularly in p53-negative cancers |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-194423 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| Other |
|
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
AR | down-regulates quantity by repression 
transcriptional regulation
|
WEE1 |
0.265 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253678 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 16281084 |
After AR antagonist flutamide treatment, three hundred and twenty-six genes (3.93%) expressed differentially, 97 down-regulated and 219 up-regulated. Among them, eight up-regulated genes might be cell cycle-related, namely CDC10, NRAS, BTG1, Wee1, CLK3, DKFZP564A122, CDKN1A and BTG2. The CDKN1A and BTG1 gene mRNA expression was confirmed to be higher in the experimental group by RT-PCR, while p53 mRNA expression had no significant changes. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CHEK1 | up-regulates
phosphorylation
|
WEE1 |
0.592 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-163164 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20068082 |
Chk1 also phosphorylates and stabilizes wee1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SCF-betaTRCP | down-regulates
binding
|
WEE1 |
0.4 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-217184 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 15340381 |
Scfb-trcp continues to have a role in this phase, however, through its induced degradation of the cdk1 inhibitor, wee1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PPP2R2A | up-regulates quantity by stabilization
dephosphorylation
|
WEE1 |
0.394 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-266381 |
|
|
Homo sapiens |
A-549 Cell |
| pmid |
sentence |
| 33108758 |
Knockout of FAM122A results in activation of PP2A-B55α, a phosphatase that dephosphorylates the WEE1 protein and rescues WEE1 from ubiquitin-mediated degradation. in tumor cells with oncogene-driven replication stress, CHK1 can directly phosphorylate FAM122A, leading to activation of the PP2A-B55α phosphatase and increased WEE1 expression. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
BTRC | down-regulates
binding
|
WEE1 |
0.406 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-128439 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 15340381 |
Scfb-trcp continues to have a role in this phase, however, through its induced degradation of the cdk1 inhibitor, wee1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
WEE1
- 
- 