| + |
MBD2 | down-regulates quantity by repression 
transcriptional regulation
|
ALOX5 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254026 |
|
|
Homo sapiens |
U-937 Cell |
| pmid |
sentence |
| 19781662 |
Human 5-lipoxygenase (5-LO) is the key enzyme in the formation of inflammatory leukotrienes. 5-LO gene expression is mainly restricted to B cells and cells of myeloid origin. It is known that basal 5-lipoxygenase promoter activity is regulated by DNA methylation.|Using ChIP assays, we found that the methyl-DNA binding proteins MBD1, MBD2 and MeCP2 bind to the methylated 5-LO core promoter in U937 cells. Knock down of each of the MBDs upregulates 5-LO mRNA expression in U937 cells indicating that these proteins are involved in silencing of the 5-LO gene. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MBD2 | form complex 
binding
|
MBD2/NuRD complex |
0.722 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-263835 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 27098840 |
The NuRD complex is a multi-protein transcriptional corepressor that couples histone deacetylase and ATP-dependent chromatin remodelling activities.In humans, an assembly of proteins called the NuRD complex makes chromatin more compact by removing acetyl groups from nucleosomes. This complex is important for early development and for the stability and repair of our genes. Three proteins make up its core: HDAC1, which removes the acetyl group from the nucleosome; MTA1, which acts as a scaffold to hold the complex together; and RBBP4, which enables the complex to interact with nucleosomes. MBD2 and MBD3 are members of the methyl cytosine-guanosine (CpG)-binding domain (MBD) family of proteins42; 43. Of the five MBD members, only MBD2 and MBD3 associate with NuRD and are required for the complex formation and gene repression. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DNMT1 | down-regulates quantity by repression
transcriptional regulation
|
MBD2 |
0.693 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254128 |
|
|
Homo sapiens |
MDA-MB-231 Cell |
| pmid |
sentence |
| 15618232 |
We then examined the levels of DNMT1 and methylated DNA-binding protein 2 (MBD2) expressions in these cells to determine whether this reduction in uPA expression is associated with changes in the DNA methylation machinery. Our results showed that ectopic expression of RAS induced DNMT1 expression and activity and inhibited MBD2 expression. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MBD2 | down-regulates quantity by repression
transcriptional regulation
|
CHD4 |
0.614 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254102 |
|
|
Mus musculus |
B-lymphocyte |
| pmid |
sentence |
| 23071088 |
MBD2 and multiple domains of CHD4 are required for transcriptional repression by Mi-2/NuRD complexes |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
3.0
MBD2
- 
- 