| + |
PRKCB | up-regulates 
phosphorylation
|
CHAT |
0.28 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129280 |
Ser464 |
LLKHVTQsSRKLIRA |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 15381704 |
Protein kinase c isoforms differentially phosphorylate human choline acetyltransferase regulating its catalytic activity. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129284 |
Ser465 |
LKHVTQSsRKLIRAD |
Homo sapiens |
|
| pmid |
sentence |
| 15381704 |
Protein kinase c isoforms differentially phosphorylate human choline acetyltransferase regulating its catalytic activity. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129288 |
Ser558 |
VPTYESAsIRRFQEG |
Homo sapiens |
|
| pmid |
sentence |
| 15381704 |
Protein kinase c isoforms differentially phosphorylate human choline acetyltransferase regulating its catalytic activity. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129292 |
Ser594 |
HKAAVPAsEKLLLLK |
Homo sapiens |
|
| pmid |
sentence |
| 15381704 |
We show that chat is differentially phosphorylated by protein kinase c (pkc) isoforms on four serines (ser-440, ser-346, ser-347, and ser-476) and one threonine (thr-255). This phosphorylation is hierarchical, with phosphorylation at ser-476 required for phosphorylation at other serines. Phosphorylation at some, but not all, sites regulates basal catalysis and activation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129296 |
Thr373 |
TVLVKDStNRDSLDM |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 15381704 |
We show that chat is differentially phosphorylated by protein kinase c (pkc) isoforms on four serines (ser-440, ser-346, ser-347, and ser-476) and one threonine (thr-255). This phosphorylation is hierarchical, with phosphorylation at ser-476 required for phosphorylation at other serines. Phosphorylation at some, but not all, sites regulates basal catalysis and activation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-96632 |
Thr373 |
TVLVKDStNRDSLDM |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 12486117 |
We show that chat is differentially phosphorylated by protein kinase c (pkc) isoforms on four serines (ser-440, ser-346, ser-347, and ser-476) and one threonine (thr-255). This phosphorylation is hierarchical, with phosphorylation at ser-476 required for phosphorylation at other serines. Phosphorylation at some, but not all, sites regulates basal catalysis and activation. |
|
| Publications: |
6 |
Organism: |
Homo Sapiens |
| Tissue: |
Brain |
| + |
PRKCE | up-regulates
phosphorylation
|
CHAT |
0.306 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129300 |
Ser464 |
LLKHVTQsSRKLIRA |
Homo sapiens |
|
| pmid |
sentence |
| 15381704 |
We show that chat is differentially phosphorylated by protein kinase c (pkc) isoforms on four serines (ser-440, ser-346, ser-347, and ser-476) and one threonine (thr-255). This phosphorylation is hierarchical, with phosphorylation at ser-476 required for phosphorylation at other serines. Phosphorylation at some, but not all, sites regulates basal catalysis and activation |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129304 |
Ser465 |
LKHVTQSsRKLIRAD |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 15381704 |
We show that chat is differentially phosphorylated by protein kinase c (pkc) isoforms on four serines (ser-440, ser-346, ser-347, and ser-476) and one threonine (thr-255). This phosphorylation is hierarchical, with phosphorylation at ser-476 required for phosphorylation at other serines. Phosphorylation at some, but not all, sites regulates basal catalysis and activation |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129308 |
Ser558 |
VPTYESAsIRRFQEG |
Homo sapiens |
|
| pmid |
sentence |
| 15381704 |
We show that chat is differentially phosphorylated by protein kinase c (pkc) isoforms on four serines (ser-440, ser-346, ser-347, and ser-476) and one threonine (thr-255). This phosphorylation is hierarchical, with phosphorylation at ser-476 required for phosphorylation at other serines. Phosphorylation at some, but not all, sites regulates basal catalysis and activation |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129312 |
Ser594 |
HKAAVPAsEKLLLLK |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 15381704 |
We show that chat is differentially phosphorylated by protein kinase c (pkc) isoforms on four serines (ser-440, ser-346, ser-347, and ser-476) and one threonine (thr-255). This phosphorylation is hierarchical, with phosphorylation at ser-476 required for phosphorylation at other serines. Phosphorylation at some, but not all, sites regulates basal catalysis and activation |
|
| Publications: |
4 |
Organism: |
Homo Sapiens |
| + |
PRKCA | up-regulates
phosphorylation
|
CHAT |
0.375 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129260 |
Ser464 |
LLKHVTQsSRKLIRA |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 15381704 |
We show that chat is differentially phosphorylated by protein kinase c (pkc) isoforms on four serines (ser-440, ser-346, ser-347, and ser-476) and one threonine (thr-255). This phosphorylation is hierarchical, with phosphorylation at ser-476 required for phosphorylation at other serines. Phosphorylation at some, but not all, sites regulates basal catalysis and activation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129264 |
Ser465 |
LKHVTQSsRKLIRAD |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 15381704 |
We show that chat is differentially phosphorylated by protein kinase c (pkc) isoforms on four serines (ser-440, ser-346, ser-347, and ser-476) and one threonine (thr-255). This phosphorylation is hierarchical, with phosphorylation at ser-476 required for phosphorylation at other serines. Phosphorylation at some, but not all, sites regulates basal catalysis and activation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129268 |
Ser558 |
VPTYESAsIRRFQEG |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 15381704 |
We show that chat is differentially phosphorylated by protein kinase c (pkc) isoforms on four serines (ser-440, ser-346, ser-347, and ser-476) and one threonine (thr-255). This phosphorylation is hierarchical, with phosphorylation at ser-476 required for phosphorylation at other serines. Phosphorylation at some, but not all, sites regulates basal catalysis and activation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129272 |
Ser594 |
HKAAVPAsEKLLLLK |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 15381704 |
We show that chat is differentially phosphorylated by protein kinase c (pkc) isoforms on four serines (ser-440, ser-346, ser-347, and ser-476) and one threonine (thr-255). This phosphorylation is hierarchical, with phosphorylation at ser-476 required for phosphorylation at other serines. Phosphorylation at some, but not all, sites regulates basal catalysis and activation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129276 |
Thr373 |
TVLVKDStNRDSLDM |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 15381704 |
We show that chat is differentially phosphorylated by protein kinase c (pkc) isoforms on four serines (ser-440, ser-346, ser-347, and ser-476) and one threonine (thr-255). This phosphorylation is hierarchical, with phosphorylation at ser-476 required for phosphorylation at other serines. Phosphorylation at some, but not all, sites regulates basal catalysis and activation. |
|
| Publications: |
5 |
Organism: |
Homo Sapiens |
| + |
PRKCG | up-regulates
phosphorylation
|
CHAT |
0.315 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129316 |
Ser464 |
LLKHVTQsSRKLIRA |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 15381704 |
We show that chat is differentially phosphorylated by protein kinase c (pkc) isoforms on four serines (ser-440, ser-346, ser-347, and ser-476) and one threonine (thr-255). This phosphorylation is hierarchical, with phosphorylation at ser-476 required for phosphorylation at other serines. Phosphorylation at some, but not all, sites regulates basal catalysis and activation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129320 |
Ser465 |
LKHVTQSsRKLIRAD |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 15381704 |
We show that chat is differentially phosphorylated by protein kinase c (pkc) isoforms on four serines (ser-440, ser-346, ser-347, and ser-476) and one threonine (thr-255). This phosphorylation is hierarchical, with phosphorylation at ser-476 required for phosphorylation at other serines. Phosphorylation at some, but not all, sites regulates basal catalysis and activation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129324 |
Ser558 |
VPTYESAsIRRFQEG |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 15381704 |
We show that chat is differentially phosphorylated by protein kinase c (pkc) isoforms on four serines (ser-440, ser-346, ser-347, and ser-476) and one threonine (thr-255). This phosphorylation is hierarchical, with phosphorylation at ser-476 required for phosphorylation at other serines. Phosphorylation at some, but not all, sites regulates basal catalysis and activation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129328 |
Ser594 |
HKAAVPAsEKLLLLK |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 15381704 |
We show that chat is differentially phosphorylated by protein kinase c (pkc) isoforms on four serines (ser-440, ser-346, ser-347, and ser-476) and one threonine (thr-255). This phosphorylation is hierarchical, with phosphorylation at ser-476 required for phosphorylation at other serines. Phosphorylation at some, but not all, sites regulates basal catalysis and activation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129332 |
Thr373 |
TVLVKDStNRDSLDM |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 15381704 |
We show that chat is differentially phosphorylated by protein kinase c (pkc) isoforms on four serines (ser-440, ser-346, ser-347, and ser-476) and one threonine (thr-255). This phosphorylation is hierarchical, with phosphorylation at ser-476 required for phosphorylation at other serines. Phosphorylation at some, but not all, sites regulates basal catalysis and activation. |
|
| Publications: |
5 |
Organism: |
Homo Sapiens |
| + |
PRKCD | up-regulates quantity
phosphorylation
|
CHAT |
0.305 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-249271 |
Ser558 |
VPTYESAsIRRFQEG |
Homo sapiens |
Neuroblast |
| pmid |
sentence |
| 15381704 |
Finally, basal ChAT phosphorylation in neurons is mediated predominantly by PKC at Ser-476, with PKC activation increasing phosphorylation at Ser-440 and enhancing ChAT activity. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-249272 |
Ser594 |
HKAAVPAsEKLLLLK |
Homo sapiens |
Neuroblast |
| pmid |
sentence |
| 15381704 |
Finally, basal ChAT phosphorylation in neurons is mediated predominantly by PKC at Ser-476, with PKC activation increasing phosphorylation at Ser-440 and enhancing ChAT activity. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
PRKCZ | up-regulates
phosphorylation
|
CHAT |
0.283 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129336 |
Ser558 |
VPTYESAsIRRFQEG |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 15381704 |
Finally, basal chat phosphorylation in neurons is mediated predominantly by pkc at ser-476, with pkc activation increasing phosphorylation at ser-440 and enhancing chat activity. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129340 |
Ser594 |
HKAAVPAsEKLLLLK |
Homo sapiens |
|
| pmid |
sentence |
| 15381704 |
Finally, basal chat phosphorylation in neurons is mediated predominantly by pkc at ser-476, with pkc activation increasing phosphorylation at ser-440 and enhancing chat activity. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
CAMK2A | up-regulates
phosphorylation
|
CHAT |
0.364 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-96628 |
Thr574 |
VDNIRSAtPEALAFV |
Homo sapiens |
Neuron |
| pmid |
sentence |
| 12486117 |
We show that chat is differentially phosphorylated by protein kinase c (pkc) isoforms on four serines (ser-440, ser-346, ser-347, and ser-476) and one threonine (thr-255). This phosphorylation is hierarchical, with phosphorylation at ser-476 required for phosphorylation at other serines. Phosphorylation at some, but not all, sites regulates basal catalysis and activation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Tissue: |
Brain |
| + |
CAMK2G | up-regulates activity
phosphorylation
|
CHAT |
0.3 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250693 |
Thr574 |
VDNIRSAtPEALAFV |
|
Neuroblastoma Cell Line |
| pmid |
sentence |
| 12486117 |
Using mass spectrometry, we identified threonine 456 as a new phosphorylation site in choline acetyltransferase from A beta-(1-42)-treated cells and in purified recombinant ChAT phosphorylated in vitro by calcium/calmodulin-dependent protein kinase II (CaM kinase II). | This phosphorylation combination was observed in choline acetyltransferase from A beta-(1-42)-treated cells. Treatment of cells with A beta-(1-42) resulted in two phases of activation of choline acetyltransferase, the first within 30 min and associated with phosphorylation by protein kinase C and the second by 10 h and associated with phosphorylation by both CaM kinase II and protein kinase C. |
|
| Publications: |
1 |
4.0
CHAT
- 
- 