| + |
MAPK1 | down-regulates activity 
phosphorylation
|
ETV3 |
0.417 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262758 |
Ser139 |
SSGVVPQsAPPVPTA |
Mus musculus |
|
| pmid |
sentence |
| 22028470 |
We have optimized a chemical genetic system using analog-sensitive ERK2, a form of ERK2 engineered to use an analog of adenosine 5'-triphosphate (ATP), to tag and isolate ERK2 substrates in vitro. This approach identified 80 proteins phosphorylated by ERK2, 13 of which are known ERK2 substrates. With this improved methodology, we detected 98 sites directly phosphorylated by ERK2 on 80 proteins from NIH 3T3-L1 fibroblasts. Thirteen of these proteins are known substrates and the rest represent previously unknown kinase/substrate interactions. Among the ERK2 substrates, we identified the E-twenty six (ETS) domain-containing protein ETV3. We determined that phosphorylation of this protein by ERK2 was functionally relevant, abrogating the DNA-binding activity of ETV3 at thousands of targets across the genome, thereby providing an additional mechanism for transcriptional regulation downstream of ERK2 activation. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
ETV3 | down-regulates quantity by repression
transcriptional regulation
|
ETV3 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262778 |
|
|
Mus musculus |
NIH-3T3 Cell |
| pmid |
sentence |
| 22028471 |
ETV3 target genes including etv3, ddx20, and dusp6 provide negative feedback regulation of ETV3 production and activity. Negative feedback along with constitutive instability may serve to tightly regulate ETV3 abundance. Our date suggest that phosphorylation by ERK2 relieves repression by ETV3, allowing activation of cell cycle control genes including myc, components of the NF-κB pathway, and genes required form RNA processing and translation. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
ETV3 | down-regulates quantity by repression
transcriptional regulation
|
DDX20 |
0.729 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262779 |
|
|
Mus musculus |
NIH-3T3 Cell |
| pmid |
sentence |
| 22028472 |
ETV3 target genes including etv3, ddx20, and dusp6 provide negative feedback regulation of ETV3 production and activity. Negative feedback along with constitutive instability may serve to tightly regulate ETV3 abundance. Our date suggest that phosphorylation by ERK2 relieves repression by ETV3, allowing activation of cell cycle control genes including myc, components of the NF-κB pathway, and genes required form RNA processing and translation. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
ETV3 | down-regulates quantity by repression
transcriptional regulation
|
DUSP6 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262780 |
|
|
Mus musculus |
NIH-3T3 Cell |
| pmid |
sentence |
| 22028473 |
ETV3 target genes including etv3, ddx20, and dusp6 provide negative feedback regulation of ETV3 production and activity. Negative feedback along with constitutive instability may serve to tightly regulate ETV3 abundance. Our date suggest that phosphorylation by ERK2 relieves repression by ETV3, allowing activation of cell cycle control genes including myc, components of the NF-κB pathway, and genes required form RNA processing and translation. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
3.0
ETV3
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