| + |
PPAT | up-regulates activity 
|
Purine biosynthesis |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-267188 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 28029518 |
The first reaction in the de novo purine biosynthetic pathway is the conversion of PRPP to 5-phosphoribosylamine (PRA) by PRPP amidotransferase (PPAT) and is presumed to be rate-limiting. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Glutamine metabolism |
| + |
PPAT | up-regulates quantity 
chemical modification
|
5-phospho-beta-D-ribosylaminium(1-) |
0.8 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-267190 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 8106516 |
Two Genes for de Novo Purine Nucleotide Synthesis on Human Chromosome 4 Are Closely Linked and Divergently Transcribed” |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-267295 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 9914248 |
Glutamine PRPP amidotransferase (GPATase) catalyzes the first step of de novo purine biosynthesis, the conversion of 5-phosphoribosyl-(~)l-pyrophosphate (PRPP) to 5-phosphoribosyl-([3)l-amine (PRA). The nitrogen source for the reaction is the amide group of glutamine. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| Pathways: | Glutamine metabolism, Nucleotide Biosynthesis |
| + |
PPAT | down-regulates quantity 
chemical modification
|
L-glutamine zwitterion |
0.8 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-267187 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 8106516 |
Two Genes for de Novo Purine Nucleotide Synthesis on Human Chromosome 4 Are Closely Linked and Divergently Transcribed” |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Glutamine metabolism, Nucleotide Biosynthesis |
| + |
PPAT | up-regulates quantity
chemical modification
|
L-glutamate(1-) |
0.8 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-267294 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 9914248 |
Glutamine PRPP amidotransferase (GPATase) catalyzes the first step of de novo purine biosynthesis, the conversion of 5-phosphoribosyl-(~)l-pyrophosphate (PRPP) to 5-phosphoribosyl-([3)l-amine (PRA). The nitrogen source for the reaction is the amide group of glutamine. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Glutamine metabolism, Nucleotide Biosynthesis |
| + |
NFE2L2 | up-regulates quantity by expression
transcriptional regulation
|
PPAT |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-267358 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22789539 |
We identified six genes involved in the PPP and NADPH production pathways as direct targets of Nrf2. To identify the target genes of NRF2 responsible for cell proliferation, we performed microarray analysis in A549 cells treated with NRF2 siRNA or control siRNA. We used three independent NRF2 siRNAs and selected genes whose expression levels were reduced to less than 66.7% of that of the control sample by all three siRNAs to minimize off-target effects (Table S1). In addition to the typical target genes of NRF2 encoding detoxifying enzymes and antioxidant proteins (cytoprotective genes), genes whose products are involved in the PPP (glucose-6-phosphate dehydrogenase [G6PD], phosphogluconate dehydrogenase [PGD], transketolase [TKT], and transaldolase 1 [TALDO1]) and de novo nucleotide synthesis (phosphoribosyl pyrophosphate amidotransferase [PPAT] and methylenetetrahydrofolate dehydrogenase 2 [MTHFD2]) were decreased by the NRF2 knockdown (Figure 1B). Genes encoding enzymes for NADPH synthesis (malic enzyme 1 [ME1] and isocitrate dehydrogenase 1 [IDH1]) were also decreased (Figure 1B). We also confirmed the reduction of the enzyme proteins encoded by these genes in the NRF2-knockdown cells (Figure 1C). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Nucleotide Biosynthesis |
| + |
PPAT | down-regulates quantity
chemical modification
|
5-phospho-α-D-ribose 1-diphosphate |
0.8 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-267293 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 9914248 |
Glutamine PRPP amidotransferase (GPATase) catalyzes the first step of de novo purine biosynthesis, the conversion of 5-phosphoribosyl-(~)l-pyrophosphate (PRPP) to 5-phosphoribosyl-([3)l-amine (PRA). The nitrogen source for the reaction is the amide group of glutamine. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Nucleotide Biosynthesis |
| + |
MYC | up-regulates quantity by expression
transcriptional regulation
|
PPAT |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-267381 |
|
|
Homo sapiens |
P493-6 Cell |
| pmid |
sentence |
| 18628958 |
PPAT, catalyzing the first step of purine synthesis, and DHODH, an enzyme generating uridine in the middle of the pyrimidine synthesis pathway, were validated as direct c-MYC target genes by all criteria. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Nucleotide Biosynthesis |
3.0
PPAT
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