| + |
FBXW10 | down-regulates quantity by destabilization 
binding
|
CBX5 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271933 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 23431138 |
As expected, the SKP1 and CUL1 proteins, subunits of all F-box-containing E3 ligases, were also present in the immune complexes containing FBXO10 and BCL2. To test for FBXO10-induced ubiquitination of BCL2, 293T cells were transduced with retroviral vectors expressing Flag-tagged FBXO10, MYC-tagged BCL2, and HA-tagged ubiquitin, and cells were treated with the proteasome inhibitor PS-341 to enhance the detection of ubiquitinated proteins.Together, these data suggest that FBXO10 is a component of a ubiquitin ligase that can target BCL2 protein for degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
FBXW10 | up-regulates activity 
binding
|
Cullin 1-RBX1-Skp1 |
0.606 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271935 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 23431138 |
As expected, the SKP1 and CUL1 proteins, subunits of all F-box-containing E3 ligases, were also present in the immune complexes containing FBXO10 and BCL2. To test for FBXO10-induced ubiquitination of BCL2, 293T cells were transduced with retroviral vectors expressing Flag-tagged FBXO10, MYC-tagged BCL2, and HA-tagged ubiquitin, and cells were treated with the proteasome inhibitor PS-341 to enhance the detection of ubiquitinated proteins.Together, these data suggest that FBXO10 is a component of a ubiquitin ligase that can target BCL2 protein for degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
FBXW10
- 
- 