| + |
PYHIN1 | down-regulates quantity by repression 
transcriptional regulation
|
HDAC1 |
0.296 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-268495 |
|
|
Homo sapiens |
MDA-MB-468 Cell |
| pmid |
sentence |
| 18247378 |
We found that maspin is selectively upregulated in IFIXα-expressing cells and involved in anti-invasive activity of IFIXα. We also present evidence indicating that IFIXα downregulates histone deacetylase 1 (HDAC1), which is possibly involved in the silencing of the maspin gene in human breast cancer cells. To confirm these results, we performed a luciferase assay using a maspin-promoter-luciferase plasmid. The results showed that HDAC1 overexpression suppressed the activity of the maspin promoter (Figure 3C). Therefore, our results suggest that IFIXα enhances maspin expression through the downregulation of HDAC1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PYHIN1 | down-regulates quantity by destabilization 
binding
|
MDM2 |
0.424 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-268493 |
|
|
Homo sapiens |
MDA-MB-468 Cell |
| pmid |
sentence |
| 16479015 |
Here, we show that IFIXalpha1 downregulates HDM2, a principal negative regulator of p53, at the posttranslational level. IFIXalpha1 destabilizes HDM2 protein and promotes its ubiquitination. The E3 ligase activity of HDM2 appears to be required for this IFIXalpha1 effect. Importantly, HDM2 downregulation is required for the IFIXalpha1-mediated increase of p53 protein levels, transcriptional activity, and nuclear localization, suggesting that IFIXalpha1 positively regulates p53 by acting as a negative regulator of HDM2. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
PYHIN1
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