| + |
PIM3 | down-regulates activity 
phosphorylation
|
BAD |
0.353 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250396 |
Ser118 |
GRELRRMsDEFVDSF |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 16403219 |
Pim kinases phosphorylate multiple sites on Bad and promote 14-3-3 binding and dissociation from Bcl-XL. pim kinases are constitutively active when expressed in HEK-293 cells and are able to phosphorylate the Bcl-2 family member Bad on three residues, Ser112, Ser136 and Ser155 in vitro and in cells. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-249605 |
Ser75 |
EIRSRHSsYPAGTED |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 16403219 |
All three Pim kinase family members predominantly phosphorylate Bad on Ser112 and in addition are capable of phosphorylating Bad on multiple sites associated with the inhibition of the pro-apoptotic function of Bad in HEK-293 cells. This would be consistent with the proposed function of Pim kinases in promoting cell proliferation and preventing cell death. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250399 |
Ser99 |
PFRGRSRsAPPNLWA |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 16403219 |
Pim kinases phosphorylate multiple sites on Bad and promote 14-3-3 binding and dissociation from Bcl-XL. pim kinases are constitutively active when expressed in HEK-293 cells and are able to phosphorylate the Bcl-2 family member Bad on three residues, Ser112, Ser136 and Ser155 in vitro and in cells. |
|
| Publications: |
3 |
Organism: |
Homo Sapiens |
| + |
PIM3 | up-regulates activity 
phosphorylation
|
PSMD2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-273897 |
Ser361 |
ENNRFGGsGSQVDSA |
in vitro |
|
| pmid |
sentence |
| 31843888 |
Seven of these kinases (PIM1/2/3, MAP4K1/2, PKA, and NEK6) directly and robustly phosphorylated recombinant GST-Rpn1 at S361 in vitro (Fig. 3D and SI Appendix, Fig. S3 A and B). |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
SGI-1776 | down-regulates
chemical inhibition
|
PIM3 |
0.8 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-206896 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| Other |
|
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PIM3 | up-regulates activity
phosphorylation
|
NFATC1 |
0.248 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276773 |
|
|
in vitro |
|
| pmid |
sentence |
| 31730483 |
In addition to PIM1, also PIM2 and PIM3 were able to phosphorylate WT, but not MM NFATC1 in vitro (Fig. (Fig.22c). |
|
| Publications: |
1 |
Organism: |
In Vitro |
3.0
PIM3
- 
- 