| + |
GPR65 | up-regulates activity 
binding
|
GNAI1 |
0.271 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272502 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 27287411 |
GPR65 is playing a critical role in phagocytic cells that require high levels of V-ATPase activity to maintain phagosomal and lysosomal pH, and this activity aids in the direct clearance of enteric pathogens. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-281553 |
|
|
Homo sapiens |
INER-51 Cell |
| pmid |
sentence |
| 33886554 |
Using this threshold G protein coupling was detected for 22 of the 48 receptors and 48 of the 240 pairings in our sample (Fig 3; S1 File). We detected constitutive coupling of 18 receptors to Gi1, 8 receptors to Gs, 6 receptors to Gq, 7 receptors to G13, and 9 receptors to G15. Of the 22 profiled receptors 11 are annotated for G protein coupling in the IUPHAR Guide to Pharmacology (GtoPdb), and within this set there was excellent agreement between our results and annotated coupling |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
GPR65 | up-regulates activity
binding
|
GNAS |
0.248 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-282066 |
|
|
Homo sapiens |
HEK-293A Cell |
| pmid |
sentence |
| 35302493 |
This study describes the development and validation of a genetically encoded ebBRET-based biosensor platform allowing live-cell mapping of GPCR-G protein coupling preferences covering 12 heterotrimeric G proteins. Profiling of 100 therapeutically relevant human GPCRs resulted in 1500 pathway-specific concentration-response curves and revealed a great diversity of coupling profiles ranging from exquisite selectivity to broad promiscuity.In our dataset, which is the first using unmodified GPCRs and Gα proteins (except for Gs), 29% of the receptors coupled to only one family, whereas others displayed more promiscuity by coupling to 2, 3, or 4 families (36%, 25%, and 10%, respectively). |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-281561 |
|
|
Homo sapiens |
HEK-293A Cell |
| pmid |
sentence |
| 33886554 |
|
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
GPR65 | up-regulates activity
binding
|
GNAO1 |
0.265 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-282002 |
|
|
Homo sapiens |
HEK-293A Cell |
| pmid |
sentence |
| 35302493 |
This study describes the development and validation of a genetically encoded ebBRET-based biosensor platform allowing live-cell mapping of GPCR-G protein coupling preferences covering 12 heterotrimeric G proteins. Profiling of 100 therapeutically relevant human GPCRs resulted in 1500 pathway-specific concentration-response curves and revealed a great diversity of coupling profiles ranging from exquisite selectivity to broad promiscuity.In our dataset, which is the first using unmodified GPCRs and Gα proteins (except for Gs), 29% of the receptors coupled to only one family, whereas others displayed more promiscuity by coupling to 2, 3, or 4 families (36%, 25%, and 10%, respectively). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
GPR65 | up-regulates activity
binding
|
GNA13 |
0.248 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-282067 |
|
|
Homo sapiens |
HEK-293A Cell |
| pmid |
sentence |
| 35302493 |
This study describes the development and validation of a genetically encoded ebBRET-based biosensor platform allowing live-cell mapping of GPCR-G protein coupling preferences covering 12 heterotrimeric G proteins. Profiling of 100 therapeutically relevant human GPCRs resulted in 1500 pathway-specific concentration-response curves and revealed a great diversity of coupling profiles ranging from exquisite selectivity to broad promiscuity.In our dataset, which is the first using unmodified GPCRs and Gα proteins (except for Gs), 29% of the receptors coupled to only one family, whereas others displayed more promiscuity by coupling to 2, 3, or 4 families (36%, 25%, and 10%, respectively). |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-281562 |
|
|
Homo sapiens |
HEK-293A Cell |
| pmid |
sentence |
| 33886554 |
|
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
4.0
GPR65
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