| + |
SRC |
phosphorylation
|
AFAP1 |
0.589 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-246351 |
Tyr451 |
TDPEALHyDYIDVEM |
Chlorocebus aethiops |
COS Cell |
| pmid |
sentence |
| 9655255 |
In this report, site-directed mutagenesis and a transient expression system that permits co-expression of activated pp60c-src (Src527F) and AFAP-110 in Cos-1 cells were used to identify the SH2-binding motif in AFAP-110. Four tyrosine residues, two in the amino terminus (Y93 and Y94) and two in the carboxy terminus (Y451 and Y453), were mutated to phenylalanine, significantly reducing overall steady-state levels of tyrosine phosphorylation and preventing Src527F from forming a stable complex with AFAP-110. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-246355 |
Tyr453 |
PEALHYDyIDVEMSA |
Chlorocebus aethiops |
|
| pmid |
sentence |
| 9655255 |
In this report, site-directed mutagenesis and a transient expression system that permits co-expression of activated pp60c-src (Src527F) and AFAP-110 in Cos-1 cells were used to identify the SH2-binding motif in AFAP-110. Four tyrosine residues, two in the amino terminus (Y93 and Y94) and two in the carboxy terminus (Y451 and Y453), were mutated to phenylalanine, significantly reducing overall steady-state levels of tyrosine phosphorylation and preventing Src527F from forming a stable complex with AFAP-110. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-246359 |
Tyr93 |
TSSLPEGyYEEAVPL |
Chlorocebus aethiops |
|
| pmid |
sentence |
| 9655255 |
In this report, site-directed mutagenesis and a transient expression system that permits co-expression of activated pp60c-src (Src527F) and AFAP-110 in Cos-1 cells were used to identify the SH2-binding motif in AFAP-110. Four tyrosine residues, two in the amino terminus (Y93 and Y94) and two in the carboxy terminus (Y451 and Y453), were mutated to phenylalanine, significantly reducing overall steady-state levels of tyrosine phosphorylation and preventing Src527F from forming a stable complex with AFAP-110. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-246363 |
Tyr94 |
SSLPEGYyEEAVPLS |
Chlorocebus aethiops |
|
| pmid |
sentence |
| 9655255 |
In this report, site-directed mutagenesis and a transient expression system that permits co-expression of activated pp60c-src (Src527F) and AFAP-110 in Cos-1 cells were used to identify the SH2-binding motif in AFAP-110. Four tyrosine residues, two in the amino terminus (Y93 and Y94) and two in the carboxy terminus (Y451 and Y453), were mutated to phenylalanine, significantly reducing overall steady-state levels of tyrosine phosphorylation and preventing Src527F from forming a stable complex with AFAP-110. |
|
| Publications: |
4 |
Organism: |
Chlorocebus Aethiops |
3.0
AFAP1
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