| + |
AURKA | up-regulates activity 
phosphorylation
|
SPAG5 |
0.525 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276648 |
Ser115 |
ISSTPKTsEEAVDPL |
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 25009111 |
We report here that Astrin acts as a mitotic phosphoprotein, and Aurora-A phosphorylates Astrin at Ser(115). The phosphorylation-deficient mutant Astrin S115A abnormally activates spindle assembly checkpoint and delays mitosis progression, decreases spindle stability, and induces chromosome misalignment. Mechanistic analyses reveal that Astrin phosphorylation mimicking mutant S115D, instead of S115A, binds and induces ubiquitination and degradation of securin, which sequentially activates Separase, an enzyme required for the separation of sister chromatids. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
GSK3B | up-regulates
phosphorylation
|
SPAG5 |
0.274 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-159574 |
Ser974 |
EESLAEMsIMTTELQ |
Homo sapiens |
|
| pmid |
sentence |
| 18055457 |
Astrin acts as a substrate for gsk3beta and is phosphorylated at thr-111, thr-937 ((s/t)p motif) and ser-974/thr-978 ((s/t)xxx(s/t)-p motif;p is a phosphorylatable residue). Inhibition of gsk3beta impairs spindle and kinetochore accumulation of astrin and spindle formation at mitosis, suggesting that astrin association with the spindle microtubule and kinetochore may be dependent on phosphorylation by gsk3beta |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-159578 |
Thr111 |
PIPQISStPKTSEEA |
Homo sapiens |
|
| pmid |
sentence |
| 18055457 |
Astrin acts as a substrate for gsk3beta and is phosphorylated at thr-111, thr-937 ((s/t)p motif) and ser-974/thr-978 ((s/t)xxx(s/t)-p motif;p is a phosphorylatable residue). Inhibition of gsk3beta impairs spindle and kinetochore accumulation of astrin and spindle formation at mitosis, suggesting that astrin association with the spindle microtubule and kinetochore may be dependent on phosphorylation by gsk3beta |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-159582 |
Thr937 |
ADEEPEStPVPLLGS |
Homo sapiens |
|
| pmid |
sentence |
| 18055457 |
Astrin acts as a substrate for gsk3beta and is phosphorylated at thr-111, thr-937 ((s/t)p motif) and ser-974/thr-978 ((s/t)xxx(s/t)-p motif;p is a phosphorylatable residue). Inhibition of gsk3beta impairs spindle and kinetochore accumulation of astrin and spindle formation at mitosis, suggesting that astrin association with the spindle microtubule and kinetochore may be dependent on phosphorylation by gsk3beta |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-159586 |
Thr978 |
AEMSIMTtELQSLCS |
Homo sapiens |
|
| pmid |
sentence |
| 18055457 |
Astrin acts as a substrate for gsk3beta and is phosphorylated at thr-111, thr-937 ((s/t)p motif) and ser-974/thr-978 ((s/t)xxx(s/t)-p motif;p is a phosphorylatable residue). Inhibition of gsk3beta impairs spindle and kinetochore accumulation of astrin and spindle formation at mitosis, suggesting that astrin association with the spindle microtubule and kinetochore may be dependent on phosphorylation by gsk3beta |
|
| Publications: |
4 |
Organism: |
Homo Sapiens |
| + |
SPAG5 | up-regulates activity 
|
CENPF |
0.345 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-252042 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 17664331 |
Furthermore, although both the core kinetochore protein Hec1 and the spindle checkpoint kinase Bub1 were unaffected (Fig. 3 C), the kinetochore resident motor protein CENP-E (Yen et al., 1992) and its interaction partner CENP-F (Chan et al., 1998) were delocalized from the kinetochore in the absence of astrin. These cells remained cyclin B1 positive (unpublished data), confirming that they were still in mitosis. These data suggest that the presence of astrin is required for the kinetochore recruitment or maintenance of CENP-E and CENP-F. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SPAG5 | up-regulates activity
relocalization
|
CDK5RAP2 |
0.478 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271719 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 26297806 |
By bringing CDK5RAP2 to the centrosome, the centriolar satellite proteins CEP72 and SPAG5 are required for the centrosomal localization of the other three MCPH proteins despite not interacting with them biochemically. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SPAG5 | up-regulates activity
|
CENPE |
0.387 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-252041 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 17664331 |
Furthermore, although both the core kinetochore protein Hec1 and the spindle checkpoint kinase Bub1 were unaffected (Fig. 3 C), the kinetochore resident motor protein CENP-E (Yen et al., 1992) and its interaction partner CENP-F (Chan et al., 1998) were delocalized from the kinetochore in the absence of astrin. These cells remained cyclin B1 positive (unpublished data), confirming that they were still in mitosis. These data suggest that the presence of astrin is required for the kinetochore recruitment or maintenance of CENP-E and CENP-F. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CDC14A | down-regulates activity 
dephosphorylation
|
SPAG5 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277066 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 27325694 |
We also demonstrate that Cdc14A dephosphorylates Astrin, and therefore the overexpression of Cdc14A sequesters Astrin in the centrosome and results in aberrant chromosome alignment. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
SPAG5
- 
- 