Relation Results

Summary

Name SPAG5
Full Name Sperm-associated antigen 5
Synonyms Astrin, Deepest, Mitotic spindle-associated protein p126, MAP126
Primary ID Q96R06
Links - -
Type protein
Relations 9
Function Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase (PubMed:11724960, PubMed:12356910, ...
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Type: Score: Layout: SPV 
0.5240.2750.20.4760.3440.385AURKASPAG5GSK3BCDC14ACDK5RAP2CENPFCENPE

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▶Modifications Tables

Relations
Regulator
Mechanism
target
score ℹ
+ AURKAup-regulates activity img/direct-activation.png phosphorylation SPAG5 0.524
Identifier Residue Sequence Organism Cell Line
SIGNOR-276648 Ser115 ISSTPKTsEEAVDPL Homo sapiens HEK-293T Cell
pmid sentence
We report here that Astrin acts as a mitotic phosphoprotein, and Aurora-A phosphorylates Astrin at Ser(115). The phosphorylation-deficient mutant Astrin S115A abnormally activates spindle assembly checkpoint and delays mitosis progression, decreases spindle stability, and induces chromosome misalignment. Mechanistic analyses reveal that Astrin phosphorylation mimicking mutant S115D, instead of S115A, binds and induces ubiquitination and degradation of securin, which sequentially activates Separase, an enzyme required for the separation of sister chromatids. 
Publications: 1 Organism: Homo Sapiens
+ GSK3Bup-regulates img/direct-activation.png phosphorylation SPAG5 0.275
Identifier Residue Sequence Organism Cell Line
SIGNOR-159574 Ser974 EESLAEMsIMTTELQ Homo sapiens
pmid sentence
Astrin acts as a substrate for gsk3beta and is phosphorylated at thr-111, thr-937 ((s/t)p motif) and ser-974/thr-978 ((s/t)xxx(s/t)-p motif;p is a phosphorylatable residue). Inhibition of gsk3beta impairs spindle and kinetochore accumulation of astrin and spindle formation at mitosis, suggesting that astrin association with the spindle microtubule and kinetochore may be dependent on phosphorylation by gsk3beta
Identifier Residue Sequence Organism Cell Line
SIGNOR-159578 Thr111 PIPQISStPKTSEEA Homo sapiens
pmid sentence
Astrin acts as a substrate for gsk3beta and is phosphorylated at thr-111, thr-937 ((s/t)p motif) and ser-974/thr-978 ((s/t)xxx(s/t)-p motif;p is a phosphorylatable residue). Inhibition of gsk3beta impairs spindle and kinetochore accumulation of astrin and spindle formation at mitosis, suggesting that astrin association with the spindle microtubule and kinetochore may be dependent on phosphorylation by gsk3beta
Identifier Residue Sequence Organism Cell Line
SIGNOR-159582 Thr937 ADEEPEStPVPLLGS Homo sapiens
pmid sentence
Astrin acts as a substrate for gsk3beta and is phosphorylated at thr-111, thr-937 ((s/t)p motif) and ser-974/thr-978 ((s/t)xxx(s/t)-p motif;p is a phosphorylatable residue). Inhibition of gsk3beta impairs spindle and kinetochore accumulation of astrin and spindle formation at mitosis, suggesting that astrin association with the spindle microtubule and kinetochore may be dependent on phosphorylation by gsk3beta
Identifier Residue Sequence Organism Cell Line
SIGNOR-159586 Thr978 AEMSIMTtELQSLCS Homo sapiens
pmid sentence
Astrin acts as a substrate for gsk3beta and is phosphorylated at thr-111, thr-937 ((s/t)p motif) and ser-974/thr-978 ((s/t)xxx(s/t)-p motif;p is a phosphorylatable residue). Inhibition of gsk3beta impairs spindle and kinetochore accumulation of astrin and spindle formation at mitosis, suggesting that astrin association with the spindle microtubule and kinetochore may be dependent on phosphorylation by gsk3beta
Publications: 4 Organism: Homo Sapiens
+ CDC14Adown-regulates activity img/direct_inhibition.png dephosphorylation SPAG5 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-277066 Homo sapiens
pmid sentence
We also demonstrate that Cdc14A dephosphorylates Astrin, and therefore the overexpression of Cdc14A sequesters Astrin in the centrosome and results in aberrant chromosome alignment.
Publications: 1 Organism: Homo Sapiens
+ SPAG5up-regulates activity img/direct-activation.png relocalization CDK5RAP2 0.476
Identifier Residue Sequence Organism Cell Line
SIGNOR-271719 Homo sapiens HeLa Cell
pmid sentence
By bringing CDK5RAP2 to the centrosome, the centriolar satellite proteins CEP72 and SPAG5 are required for the centrosomal localization of the other three MCPH proteins despite not interacting with them biochemically.
Publications: 1 Organism: Homo Sapiens
+ SPAG5up-regulates activity img/indirect-activation.png CENPF 0.344
Identifier Residue Sequence Organism Cell Line
SIGNOR-252042 Homo sapiens HeLa Cell
pmid sentence
Furthermore, although both the core kinetochore protein Hec1 and the spindle checkpoint kinase Bub1 were unaffected (Fig. 3 C), the kinetochore resident motor protein CENP-E (Yen et al., 1992) and its interaction partner CENP-F (Chan et al., 1998) were delocalized from the kinetochore in the absence of astrin. These cells remained cyclin B1 positive (unpublished data), confirming that they were still in mitosis. These data suggest that the presence of astrin is required for the kinetochore recruitment or maintenance of CENP-E and CENP-F.
Publications: 1 Organism: Homo Sapiens
+ SPAG5up-regulates activity img/indirect-activation.png CENPE 0.385
Identifier Residue Sequence Organism Cell Line
SIGNOR-252041 Homo sapiens HeLa Cell
pmid sentence
Furthermore, although both the core kinetochore protein Hec1 and the spindle checkpoint kinase Bub1 were unaffected (Fig. 3 C), the kinetochore resident motor protein CENP-E (Yen et al., 1992) and its interaction partner CENP-F (Chan et al., 1998) were delocalized from the kinetochore in the absence of astrin. These cells remained cyclin B1 positive (unpublished data), confirming that they were still in mitosis. These data suggest that the presence of astrin is required for the kinetochore recruitment or maintenance of CENP-E and CENP-F.
Publications: 1 Organism: Homo Sapiens
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