+ |
PIP3 | up-regulates quantity
binding
|
RPS6KC1 |
0.8 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-273743 |
|
|
Chlorocebus aethiops |
COS-7 Cell |
pmid |
sentence |
12077123 |
We have shown here that a newly identified protein, RPK118, can bind to and co-localize with SPHK1 in COS7-cells. As shown in Fig.8, RPK118 exhibited specific binding to PtdIns (3)P compared with other phosphoinositides. RPK118 Interacts Specifically with Phosphatidylinositol 3-Phosphate (PtdIns (3)P) through Its PX Domain. In vitro protein kinase assay showing that RPK118 has no protein kinase activity. |
|
Publications: |
1 |
Organism: |
Chlorocebus Aethiops |
+ |
RPS6KC1 | up-regulates activity
binding
|
SPHK1 |
0.392 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-273744 |
|
|
Chlorocebus aethiops |
COS-7 Cell |
pmid |
sentence |
12077123 |
The PSK2 domain of RPK118 is required for binding to SPHK1 as demonstrated by the results from immunoprecipitation analyses (Fig. 6) as well as yeast two-hybrid screening (Fig. 1A). The overexpression of RPK118 in COS7 cells did not cause any change in the intracellular content of SPP with repeated experiments, and RPK118 binding to SPHK1 did not alter the enzymatic activity of SPHK1 in vitro (data not shown), suggesting that RPK118 may function only as an adaptor molecule for SPHK1 |
|
Publications: |
1 |
Organism: |
Chlorocebus Aethiops |