| + |
MUL1 | up-regulates activity 
sumoylation
|
DNM1L |
0.55 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-274128 |
Lys594 |
GNWRGMLKTSKAEEL |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 19638400 |
Through a detailed analysis, we find that Drp1 interacts with the SUMO-conjugating enzyme Ubc9 via multiple regions and demonstrate that Drp1 is a direct target of SUMO modification by all three SUMO isoforms. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-274129 |
Lys606 |
EELLAEEKSKPIPIM |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 19638400 |
Through detailed analysis, we find that Drp1 interacts with the SUMO-conjugating enzyme Ubc9 via multiple regions and demonstrate that Drp1 is a direct target of SUMO modification by all three SUMO isoforms. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-274130 |
Lys608 |
LLAEEKSKPIPIMPA |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 19638400 |
Through detailed analysis, we find that Drp1 interacts with the SUMO-conjugating enzyme Ubc9 via multiple regions and demonstrate that Drp1 is a direct target of SUMO modification by all three SUMO isoforms. |
|
| Publications: |
3 |
Organism: |
Homo Sapiens |
| + |
GSK3B | up-regulates activity
phosphorylation
|
DNM1L |
0.397 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276849 |
Ser40 |
TQSSGKSsVLESLVG |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 25192600 |
We identified glycogen synthase kinase (GSK)3β-dependent Drp1 phosphorylation at Ser(40) and Ser(44), which increases Drp1 GTPase activity and its mitochondrial distribution and could induce mitochondrial fragmentation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276848 |
Ser44 |
GKSSVLEsLVGRDLL |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 25192600 |
We identified glycogen synthase kinase (GSK)3β-dependent Drp1 phosphorylation at Ser(40) and Ser(44), which increases Drp1 GTPase activity and its mitochondrial distribution and could induce mitochondrial fragmentation. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
CyclinB/CDK1 | up-regulates activity
phosphorylation
|
DNM1L |
0.379 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-274131 |
Ser585 |
WRGMLKTSKAEELLA |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 17301055 |
Drp1 was specifically phosphorylated at Ser-585 by Cdk1/cyclin B, which stimulated the mitochondrial fission in mitosis. From these results, we concluded that mitochondrial morphology is regulated by Drp1-dependent mitochondrial fission in mitotic cells |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MTOR | up-regulates activity
phosphorylation
|
DNM1L |
0.352 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-275430 |
Ser616 |
PIPIMPAsPQKGHAV |
Homo sapiens |
JURKAT Cell |
| pmid |
sentence |
| 34535949 |
Furthermore, we confirmed also in Jurkat cells that the specific silencing of both ERK1/2 and mTOR by siRNA downregulates Drp1 phosphorylation on Ser616 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PRKCD | up-regulates
phosphorylation
|
DNM1L |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-153148 |
Ser616 |
PIPIMPAsPQKGHAV |
Homo sapiens |
|
| pmid |
sentence |
| 17301055 |
Drp1 was specifically phosphorylated in mitosis by cdk1/cyclin b on ser-585. Exogenous expression of unphosphorylated mutant drp1s585a led to reduced mitotic mitochondrial fragmentation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ERK1/2 | up-regulates activity
phosphorylation
|
DNM1L |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-275407 |
Ser616 |
PIPIMPAsPQKGHAV |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 25658205 |
Here, we show that expression of oncogenic Ras or direct activation of the MAPK pathway leads to increased mitochondrial fragmentation and that blocking this phenotype, through knockdown of the mitochondrial fission-mediating GTPase Drp1, inhibits tumor growth. This fission is driven by Erk2-mediated phosphorylation of Drp1 on Serine 616, and both this phosphorylation and mitochondrial fragmentation are increased in human pancreatic cancer. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PPP3CB | up-regulates activity
dephosphorylation
|
DNM1L |
0.281 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-248361 |
Ser637 |
VPVARKLsAREQRDC |
Homo sapiens |
|
| pmid |
sentence |
| 18838687 |
When mitochondrial depolarization is associated with sustained cytosolic Ca(2+) rise, it activates the cytosolic phosphatase calcineurin that normally interacts with Drp1. Calcineurin-dependent dephosphorylation of Drp1, and in particular of its conserved serine 637, regulates its translocation to mitochondria as substantiated by site directed mutagenesis. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ROCK1 | up-regulates activity
phosphorylation
|
DNM1L |
0.319 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262549 |
Ser637 |
VPVARKLsAREQRDC |
Mus musculus |
|
| pmid |
sentence |
| 31063459 |
We have also previously reported that ROCK1-mediated Drp1S600 phosphorylation resulted in enhanced mitochondrial fission in podocytes |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
CAMK1 | up-regulates activity
phosphorylation
|
DNM1L |
0.34 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262552 |
Ser637 |
VPVARKLsAREQRDC |
|
|
| pmid |
sentence |
| 31063459 |
For example, protein kinase A (PKA) phosphorylation of Drp1S600 has been reported to decrease Drp1 GTPase activity in vitro (23, 24), whereas phosphorylation of the same conserved serine residue by Ca2+-calmodulin–dependent protein kinase Iα (CaMKIα) in Drp1 isoform 3 has been reported to cause a significant increase in mitochondrial fission |
|
| Publications: |
1 |
| + |
PPP3CA | up-regulates activity
dephosphorylation
|
DNM1L |
0.28 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-248676 |
Ser637 |
VPVARKLsAREQRDC |
Homo sapiens |
|
| pmid |
sentence |
| 18838687 |
When mitochondrial depolarization is associated with sustained cytosolic Ca(2+) rise, it activates the cytosolic phosphatase calcineurin that normally interacts with Drp1. Calcineurin-dependent dephosphorylation of Drp1, and in particular of its conserved serine 637, regulates its translocation to mitochondria as substantiated by site directed mutagenesis. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PPP3CC | up-regulates activity
dephosphorylation
|
DNM1L |
0.265 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-248506 |
Ser637 |
VPVARKLsAREQRDC |
Homo sapiens |
|
| pmid |
sentence |
| 18838687 |
When mitochondrial depolarization is associated with sustained cytosolic Ca(2+) rise, it activates the cytosolic phosphatase calcineurin that normally interacts with Drp1. Calcineurin-dependent dephosphorylation of Drp1, and in particular of its conserved serine 637, regulates its translocation to mitochondria as substantiated by site directed mutagenesis. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PKA | down-regulates activity 
phosphorylation
|
DNM1L |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262551 |
Ser637 |
VPVARKLsAREQRDC |
in vitro |
|
| pmid |
sentence |
| 31063459 |
For example, protein kinase A (PKA) phosphorylation of Drp1S600 has been reported to decrease Drp1 GTPase activity in vitro (23, 24), whereas phosphorylation of the same conserved serine residue by Ca2+-calmodulin–dependent protein kinase Iα (CaMKIα) in Drp1 isoform 3 has been reported to cause a significant increase in mitochondrial fission |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
Calcineurin | up-regulates activity
dephosphorylation
|
DNM1L |
0.275 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-252315 |
Ser637 |
VPVARKLsAREQRDC |
Homo sapiens |
|
| pmid |
sentence |
| 18838687 |
When mitochondrial depolarization is associated with sustained cytosolic Ca(2+) rise, it activates the cytosolic phosphatase calcineurin that normally interacts with Drp1. Calcineurin-dependent dephosphorylation of Drp1, and in particular of its conserved serine 637, regulates its translocation to mitochondria as substantiated by site directed mutagenesis. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
GSK3B | down-regulates activity
phosphorylation
|
DNM1L |
0.397 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276430 |
Ser693 |
LVGQLYKsSLLDDLL |
in vitro |
|
| pmid |
sentence |
| 23185298 |
After identifying Ser693 as a GSK3beta phosphorylation site, we also determined that K679 is crucial for GSK3beta-binding, which strongly suggests that Drp1 is a novel substrate for GSK3beta. Our results suggest that GSK3beta-mediated phosphorylation at Ser693 does cause a dramatic decrease of GTPase activity; in contrast, GSK3beta-mediated phosphorylation at Ser693 appears not to affect Drp1 inter-/intra-molecular interactions. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
LRRK2 | up-regulates activity
phosphorylation
|
DNM1L |
0.585 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276495 |
Thr595 |
NWRGMLKtSKAEELL |
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 23813973 |
LRRK2 G2019S directly bound to and phosphorylated Drp1 at Threonine595, whereas P110 treatment abolished this phosphorylation.Threonine595 phosphorylation of Drp1 by LRRK2 G2019S is required for Drp1-mediated mitochondrial fragmentation and excessive autophagy |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ABL1 | up-regulates activity
phosphorylation
|
DNM1L |
0.263 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277328 |
Tyr266 |
TDSIRDEyAFLQKKY |
Homo sapiens |
SH-SY5Y Cell |
| pmid |
sentence |
| 29022905 |
In this study, we found that c-Abl phosphorylated Drp1 at tyrosine 266, 368 and 449 in vitro and in vivo, which augmented the GTPase activity of Drp1 and promoted Drp1-mediated mitochondrial fragmentation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277329 |
Tyr368 |
CGGARICyIFHETFG |
Homo sapiens |
SH-SY5Y Cell |
| pmid |
sentence |
| 29022905 |
In this study, we found that c-Abl phosphorylated Drp1 at tyrosine 266, 368 and 449 in vitro and in vivo, which augmented the GTPase activity of Drp1 and promoted Drp1-mediated mitochondrial fragmentation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277327 |
Tyr449 |
IIQHCSNySTQELLR |
Homo sapiens |
SH-SY5Y Cell |
| pmid |
sentence |
| 29022905 |
In this study, we found that c-Abl phosphorylated Drp1 at tyrosine 266, 368 and 449 in vitro and in vivo, which augmented the GTPase activity of Drp1 and promoted Drp1-mediated mitochondrial fragmentation. |
|
| Publications: |
3 |
Organism: |
Homo Sapiens |
| + |
MTFP1 | up-regulates activity 
relocalization
|
DNM1L |
0.406 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-275449 |
|
|
Mus musculus |
MEF Cell |
| pmid |
sentence |
| 28918902 |
Regardless of the precise mechanism, our data establish MTFP1 as an essential regulator of mitochondrial fission through the modulation of DRP1 phosphorylation and recruitment to the mitochondrion. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
FZR1 | down-regulates quantity
ubiquitination
|
DNM1L |
0.356 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-274127 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 21325626 |
Here we demonstrate that changes in mitochondrial dynamics as cells exit mitosis are driven in part through ubiquitylation of Drp1 catalyzed by the APC/Ccdh1 (anaphase-promoting complex/cyclosome and its coactivator (Cdh1) E3 ubiquiting ligase complex |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MFF | up-regulates activity
relocalization
|
DNM1L |
0.621 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-245957 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21149567 |
Mff functions as an essential factor in mitochondrial recruitment of Drp1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PDCD1 | down-regulates activity 
|
DNM1L |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-275406 |
|
|
Homo sapiens |
T-lymphocyte |
| pmid |
sentence |
| 34535949 |
Mechanistically, we provided evidence that PD1 signaling downregulates Drp1 activating phosphorylation on Ser616 (and consequently mitochondrial fragmentation) via the inhibition of ERK1/2 and mTOR kinases. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
nitric oxide | up-regulates activity
|
DNM1L |
0.8 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-275650 |
|
|
|
|
| pmid |
sentence |
| 33850055 |
Upon viral infection, DDAH2 relocated to mitochondria, where it induced the production of nitric oxide (NO) and the activation of dynamin-related protein 1 (Drp1) |
|
| Publications: |
1 |
| + |
PP2B | up-regulates activity
dephosphorylation
|
DNM1L |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269994 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 18838687 |
When mitochondrial depolarization is associated with sustained cytosolic Ca(2+) rise, it activates the cytosolic phosphatase calcineurin that normally interacts with Drp1. Calcineurin-dependent dephosphorylation of Drp1, and in particular of its conserved serine 637, regulates its translocation to mitochondria as substantiated by site directed mutagenesis. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DNM1L | up-regulates
|
Mitochondrial_fission |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-274132 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 17301055 |
Mitotic phosphorylation of dynamin-related GTPase Drp1 participates in mitochondrial fission |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272975 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 25486875 |
During fission, DRP1 is recruited from the cytosol to the outer mitochondrial membrane, where it assembles with FIS1 to constrict the mitochondrial tubule (2) |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262553 |
|
|
Mus musculus |
Podocyte |
| pmid |
sentence |
| 31063459 |
Mitochondrial fission is governed primarily by the cytoplasmic dynamin-related protein 1 (Drp1). Drp1 is a conserved dynamin GTPase superfamily protein that is translocated from its cytoplasmic pool to the outer mitochondrial membrane, where Drp1 then assembles into constrictive ring-like multimeric structures, ultimately driving mitochondrial fragmentation through a GTP-dependent mechanism|We have also previously reported that ROCK1-mediated Drp1S600 phosphorylation resulted in enhanced mitochondrial fission in podocytes |
|
| Publications: |
3 |
Organism: |
Homo Sapiens, Mus Musculus |
| + |
DNM1L | up-regulates activity
binding
|
ARP2/3 |
0.287 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262550 |
|
|
Mus musculus |
Podocyte |
| pmid |
sentence |
| 31063459 |
Importantly, we found that crosstalk between phosphorylated Drp1S600 (p-Drp1S600) and the actin-binding protein com- plex Arp2/3 is a required step in mitochondrial Drp1 recruitment and mitochondrial fission under HG conditions. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
MARCHF5 | down-regulates quantity by destabilization
polyubiquitination
|
DNM1L |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271894 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 16874301 |
We found that MITOL associated with and ubiquitinated mitochondrial fission protein hFis1 and Drp1.Pulse–chase experiment also indicated that MITOL overexpression promoted Drp1 turnover. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
DNM1L
- 
- 