| + |
CDK2 | down-regulates activity 
phosphorylation
|
FZR1 |
0.742 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250732 |
Ser151 |
DVSPYSLsPVSNKSQ |
|
HEK-293 Cell |
| pmid |
sentence |
| 12560341 |
A nuclear localization signal conserved in various species was identified in CDH1, and it sufficiently targets green fluorescent protein to the nucleus. Interestingly, a CDH1-4D mutant mimicking the hyperphosphorylated form was constitutively found in the cytoplasm. In further support of the notion that phosphorylation inhibits nuclear import, the nuclear localization signal of CDH1 with two phospho-accepting serine/threonine residues changed into aspartates was unable to drive heterologous protein into the nucleus. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250733 |
Ser163 |
KSQKLLRsPRKPTRK |
|
|
| pmid |
sentence |
| 12560341 |
A nuclear localization signal conserved in various species was identified in CDH1, and it sufficiently targets green fluorescent protein to the nucleus. Interestingly, a CDH1-4D mutant mimicking the hyperphosphorylated form was constitutively found in the cytoplasm. In further support of the notion that phosphorylation inhibits nuclear import, the nuclear localization signal of CDH1 with two phospho-accepting serine/threonine residues changed into aspartates was unable to drive heterologous protein into the nucleus. |
|
| Publications: |
2 |
| + |
CCNF | down-regulates quantity by destabilization
ubiquitination
|
FZR1 |
0.533 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-266363 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 27653696 |
We show that cyclin F, a cell-cycle-regulated substrate receptor (F-box protein) for the SCF, is targeted for degradation by APC/C. Furthermore, we establish that Cdh1 is itself a substrate of SCF(cyclin F). Cyclin F loss impairs Cdh1 degradation and delays S-phase entry, and this delay is reversed by simultaneous removal of Cdh1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
USP37 | down-regulates activity
binding
|
FZR1 |
0.337 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-265054 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 21596315 |
Here we show that USP37 binds the APC/C coactivator CDH1|Deubiquitinase USP37 is activated by CDK2 to antagonize APC(CDH1) and promote S phase entry |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PIM1 | down-regulates activity
phosphorylation
|
FZR1 |
0.317 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-259820 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 20663873 |
Pim-1 phosphorylates Cdh1 and impairs binding of this protein to another APC/C complex member, CDC27. These modifications inhibit Skp2 from degradation.Pim-1 Impairs Cdh1 and CDC27 Interaction and Phosphorylates Cdh1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
FZR1 | down-regulates quantity by destabilization
binding
|
REV1 |
0.242 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272893 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 23287467 |
Here, we show that human REV1 undergoes proteosomal degradation mediated by the E3 ubiquitin ligase known as anaphase-promoting complex (APC). REV1 associates with APC. Overexpression of APC coactivator CDH1 or CDC20 promotes polyubiquitination and proteosomal degradation of REV1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
FZR1 | down-regulates quantity by destabilization
binding
|
ANLN |
0.267 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272654 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 16040610 |
Ubiquitination of anillin required a destruction-box and was mediated by Cdh1, an activator of APC/C. Overexpression of Cdh1 reduced the levels of anillin, whereas inactivation of APC/C(Cdh1) increased the half-life of anillin. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
FZR1 | up-regulates activity 
binding
|
UBE2S |
0.757 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-265081 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19822757 |
Ube2S depends on the cell cycle-dependent association with the APC/C activators Cdc20 and Cdh1 for its activity |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
FBXO5 | down-regulates
ubiquitination
|
FZR1 |
0.754 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-113385 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 11751633 |
Emi1 binds cdh1 and inhibits apc-cdh1 activity. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
FZR1 | up-regulates activity
binding
|
APC-c |
0.85 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272611 |
|
|
Chlorocebus aethiops |
COS Cell |
| pmid |
sentence |
| 12023018 |
We previously showed that human Aurora-A is turned over through the anaphase promoting complex/cyclosome (APC/C)–ubiquitin–proteasome pathway. The association of two distinct WD40 repeat proteins known as Cdc20 and Cdh1, respectively, sequentially activates the APC/C. The present study shows that Aurora-A degradation is dependent on hCdh1 in vivo, not on hCdc20, and that Aurora-A is targeted for proteolysis through distinct structural features of the destruction box, the KEN box motifs and its kinase activity. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-252015 |
|
|
|
|
| pmid |
sentence |
| 16896351 |
In addition to E2 enzymes, APC/C activity is also strictly dependent on one of several co-activator proteins that associate with APC/C during specific periods of the cell cycle. The best studied of these are Cdc20 and Cdh1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272897 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 23287467 |
Here, we show that human REV1 undergoes proteosomal degradation mediated by the E3 ubiquitin ligase known as anaphase-promoting complex (APC). REV1 associates with APC. Overexpression of APC coactivator CDH1 or CDC20 promotes polyubiquitination and proteosomal degradation of REV1. |
|
| Publications: |
3 |
Organism: |
Chlorocebus Aethiops, , Homo Sapiens |
| + |
FZR1 | down-regulates quantity by destabilization
binding
|
SKIL |
0.386 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272621 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 11741538 |
We demonstrate that the anaphase-promoting complex (APC) is a ubiquitin ligase required for the destruction of SnoN and that the APC pathway is regulated by TGF-beta. The destruction box of SnoN is required for its degradation in response to TGF-beta signaling. Furthermore, the APC activator CDH1 and Smad3 synergistically regulate SnoN degradation. Under these circumstances, CDH1 forms a quaternary complex with SnoN, Smad3, and APC. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
FZR1 | down-regulates quantity by destabilization
binding
|
PFKFB3 |
0.264 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271436 |
|
|
Homo sapiens |
SH-SY5Y Cell |
| pmid |
sentence |
| 20080744 |
We have recently discovered that the glycolysis-promoting enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, isoform 3 (PFKFB3), is degraded by the E3 ubiquitin ligase APC/C-Cdh1, which also degrades cell-cycle proteins. Cdh1 promotes PFKFB3 degradation in the nucleus. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
FZR1 | down-regulates quantity by destabilization
ubiquitination
|
CDC25A |
0.466 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271388 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 12234927 |
We found that Cdc25 A degradation during mitotic exit and in early G(1) is mediated by the anaphase-promoting complex or cyclosome (APC/C)(Cdh1) ligase, and that a KEN-box motif in the N-terminus of the protein is required for its targeted degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
FZR1 | down-regulates quantity by destabilization
binding
|
TK1 |
0.342 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272945 |
|
|
in vitro |
|
| pmid |
sentence |
| 14701726 |
We show that hTK1 is degraded via a ubiquitin-proteasome pathway in mammalian cells and that anaphase-promoting complex/cyclosome (APC/C) activator Cdh1 is not only a necessary but also a rate-limiting factor for mitotic degradation of hTK1. By in vitro ubiquitinylation assays, we demonstrated that hTK1 is targeted for degradation by the APC/C-Cdh1 ubiquitin ligase dependent on this KEN box motif. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
FZR1 | down-regulates quantity
ubiquitination
|
DNM1L |
0.35 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-274127 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 21325626 |
Here we demonstrate that changes in mitochondrial dynamics as cells exit mitosis are driven in part through ubiquitylation of Drp1 catalyzed by the APC/Ccdh1 (anaphase-promoting complex/cyclosome and its coactivator (Cdh1) E3 ubiquiting ligase complex |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
FZR1 | down-regulates quantity by destabilization
binding
|
DTYMK |
0.226 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272652 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 16103219 |
We demonstrate that TMPK is recognized and degraded by APC/C-Cdc20/Cdh1-mediated pathways from mitosis to the early G1 phase, whereas TK1 is targeted for degradation by APC/C-Cdh1 after mitotic exit. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
FZR1 | down-regulates quantity by destabilization
binding
|
MOAP1 |
0.299 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272913 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 22529100 |
MOAP-1 is an APC/CCdh1 substrate. Here, we identify MOAP-1 as a novel APC/CCdh1 substrate. MOAP-1 is degraded during G1 by APC/CCdh1, and this degradation is inhibited by Trim39 acting on the APC/C. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
FZR1 | down-regulates quantity by destabilization
binding
|
AURKA |
0.558 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272610 |
|
|
Chlorocebus aethiops |
COS Cell |
| pmid |
sentence |
| 12023018 |
We previously showed that human Aurora-A is turned over through the anaphase promoting complex/cyclosome (APC/C)–ubiquitin–proteasome pathway. The association of two distinct WD40 repeat proteins known as Cdc20 and Cdh1, respectively, sequentially activates the APC/C. The present study shows that Aurora-A degradation is dependent on hCdh1 in vivo, not on hCdc20, and that Aurora-A is targeted for proteolysis through distinct structural features of the destruction box, the KEN box motifs and its kinase activity. |
|
| Publications: |
1 |
Organism: |
Chlorocebus Aethiops |
| + |
MAD2L2 | down-regulates activity
binding
|
FZR1 |
0.541 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264902 |
|
|
in vitro |
|
| pmid |
sentence |
| 11459826 |
The APC is activated in mitosis and G1 by CDC20 and CDH1, and inhibited by the checkpoint protein MAD2, a specific inhibitor of CDC20. We show here that a MAD2 homolog MAD2B also inhibits APC. MAD2B directly inhibits activation of APC by CDC20 and CDH1 |
|
| Publications: |
1 |
Organism: |
In Vitro |
3.0
FZR1
- 
- 