| + |
PDHX | up-regulates quantity by expression 
transcriptional regulation
|
INS |
0.291 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254902 |
|
|
Homo sapiens |
Glucagonoma Cell |
| pmid |
sentence |
| 12783165 |
In glucagonoma cells transduced with a Pdx1-encoding lentiviral vector, insulin gene expression was induced while glucagon mRNA levels were reduced by 50 to 60%. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PDHX | down-regulates activity 
binding
|
CDX2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254904 |
|
|
Homo sapiens |
BHK-21 Cell |
| pmid |
sentence |
| 12783165 |
In the heterologous cell line BHK-21, Pdx1 inhibited by 60 to 80% the activation of the alpha-cell specific element G1 conferred by Pax-6 and/or Cdx-2/3. Although Pdx1 could bind three AT-rich motifs within G1, two of which are binding sites for Pax-6 and Cdx-2/3, the affinity of Pdx1 for G1 was much lower as compared to Pax-6. In addition, Pdx1 inhibited Pax-6 mediated activation through G3, to which Pdx1 was unable to bind. Moreover, a mutation impairing DNA binding of Pdx1 had no effect on its inhibition on Cdx-2/3. Since Pdx1 interacts directly with Pax-6 and Cdx-2/3 forming heterodimers, we suggest that Pdx1 inhibits glucagon gene transcription through protein to protein interactions with Pax-6 and Cdx-2/3. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PDHX | down-regulates quantity by repression 
transcriptional regulation
|
GCG |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254901 |
|
|
Homo sapiens |
Glucagonoma Cell |
| pmid |
sentence |
| 12783165 |
In glucagonoma cells transduced with a Pdx1-encoding lentiviral vector, insulin gene expression was induced while glucagon mRNA levels were reduced by 50 to 60%. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PDHX | form complex 
binding
|
PDH |
0.812 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-266543 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20160912 |
The human (h) pyruvate dehydrogenase complex (hPDC) consists of multiple copies of several components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2), dihydrolipoamide dehydrogenase (E3), E3-binding protein (BP), and specific kinases and phosphatases. Mammalian PDC has a well organized structure with an icosahedral symmetry of the central E2/BP core to which the other component proteins bind non-covalently. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
FOXO1 | down-regulates quantity by repression
transcriptional regulation
|
PDHX |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278151 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 12488434 |
Our genetic analysis indicates that Foxo1 is an effector of Irs2 signaling in pancreatic β cells. Foxo1 inactivation leads to increased Pdx1 expression and β cell proliferation. Since Foxo1 is expressed in a subset of cells embedded within pancreatic ducts, we propose that, in quiescent duct-associated cells that are not committed to a β cell fate, Foxo1 acts as a transcriptional brake on Pdx1. We propose the following mechanism of Foxo1 regulation: small quantities of insulin are released in the pancreatic duct (31), where they activate signaling (32) in the Foxo1-positive duct cell subset, leading to Foxo1 nuclear exclusion and Pdx1 expression. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PDHX | down-regulates activity
binding
|
PAX6 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254903 |
|
|
Homo sapiens |
BHK-21 Cell |
| pmid |
sentence |
| 12783165 |
In the heterologous cell line BHK-21, Pdx1 inhibited by 60 to 80% the activation of the alpha-cell specific element G1 conferred by Pax-6 and/or Cdx-2/3. Although Pdx1 could bind three AT-rich motifs within G1, two of which are binding sites for Pax-6 and Cdx-2/3, the affinity of Pdx1 for G1 was much lower as compared to Pax-6. In addition, Pdx1 inhibited Pax-6 mediated activation through G3, to which Pdx1 was unable to bind. Moreover, a mutation impairing DNA binding of Pdx1 had no effect on its inhibition on Cdx-2/3. Since Pdx1 interacts directly with Pax-6 and Cdx-2/3 forming heterodimers, we suggest that Pdx1 inhibits glucagon gene transcription through protein to protein interactions with Pax-6 and Cdx-2/3. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
PDHX
- 
- 