| + |
AMPK | down-regulates activity 
phosphorylation
|
SLC12A2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276630 |
Ser77 |
RPLGPTPsQSRFQVD |
in vitro |
|
| pmid |
sentence |
| 24702155 |
AMPK was found to directly phosphorylate a recombinant human-NKCC1 N-terminal fragment (1-293) with the phosphorylated site identified as S77. Mutation of Serine 77 to Alanine partially prevented the inhibitory effect of A-769662 on NKCC1 activity. In conclusion, AMPK can act to reduce NKCC1-mediated transport. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
OXSR1 | up-regulates 
phosphorylation
|
SLC12A2 |
0.54 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-146513 |
Thr203 |
HQHYYYDtHTNTYYL |
Homo sapiens |
|
| pmid |
sentence |
| 16669787 |
We have identified three residues in nkcc1 (thr175/thr179/thr184 in shark or thr203/thr207/thr212 in human) that are phosphorylated by spak and osr1 / exposure of hek-293 (human embryonic kidney) cells to osmotic stress, which leads to phosphorylation and activation of nkcc1, increased phosphorylation of nkcc1 at the sites targeted by spak/osr1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-90927 |
Thr203 |
HQHYYYDtHTNTYYL |
Homo sapiens |
|
| pmid |
sentence |
| 12145304 |
The secretory na-k-cl cotransporter nkcc1 is activated by secretagogues through a phosphorylation-dependent mechanism. three phosphoacceptor sites were identified in the n-terminal domain of the protein (at thr184, thr189, and thr202) none of these residues occurs in the context of strong consensus sites for known ser/thr kinases. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-146517 |
Thr207 |
YYDTHTNtYYLRTFG |
Homo sapiens |
|
| pmid |
sentence |
| 16669787 |
We have identified three residues in nkcc1 (thr175/thr179/thr184 in shark or thr203/thr207/thr212 in human) that are phosphorylated by spak and osr1 / exposure of hek-293 (human embryonic kidney) cells to osmotic stress, which leads to phosphorylation and activation of nkcc1, increased phosphorylation of nkcc1 at the sites targeted by spak/osr1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-146521 |
Thr212 |
TNTYYLRtFGHNTMD |
Homo sapiens |
|
| pmid |
sentence |
| 16669787 |
We have identified three residues in nkcc1 (thr175/thr179/thr184 in shark or thr203/thr207/thr212 in human) that are phosphorylated by spak and osr1 / exposure of hek-293 (human embryonic kidney) cells to osmotic stress, which leads to phosphorylation and activation of nkcc1, increased phosphorylation of nkcc1 at the sites targeted by spak/osr1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-90931 |
Thr212 |
TNTYYLRtFGHNTMD |
Homo sapiens |
|
| pmid |
sentence |
| 12145304 |
Oxidative stress-responsive kinase-1 (osr1) is a known upstream regulator of n(k)ccs. these results suggest that, globally, osr1 is involved in the regulation of bp and renal tubular na(+) reabsorption mainly via the activation of nkcc1 and nkcc2. |
|
| Publications: |
5 |
Organism: |
Homo Sapiens |
| + |
SLC12A2 | up-regulates quantity
phosphorylation, relocalization
|
chloride |
0.8 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264634 |
|
|
|
|
| pmid |
sentence |
| 21613606 |
Eukaryotic cells regulate their volume in the long term through the coordinated function of the Na+-coupled chloride (NKCC1/2 and NCC) and K+-coupled chloride (KCC1–4) cotransporters, which encompass two branches of the SLC12|The K+-Cl− cotransporters move chloride outside the cell, are inhibited by phosphorylation, and are activated by dephosphorylation. In contrast, the Na+-K+-2Cl− cotransporters introduce chloride into the cell, are inhibited by dephosphorylation, and are activated by phosphorylation gene family of solute transporters (12). |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264986 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 26951057 |
As shown in Fig. 2, the intracellular Cl− concentration is regulated mainly by two cation-chloride cotransporters, NKCC1 and KCC2 [32]. NKCC1 imports Cl− whereas KCC2 extrudes intracellular Cl−. |
|
| Publications: |
2 |
Organism: |
, Homo Sapiens |
| Pathways: | GABAergic synapse |
| + |
STK39 | up-regulates activity
phosphorylation
|
SLC12A2 |
0.62 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264642 |
|
|
|
|
| pmid |
sentence |
| 16990453 |
This phosphorylation event activates PASK, which in turn phosphorylates and activates NKCC1 |
|
| Publications: |
1 |
| + |
WNK3 | up-regulates activity
phosphorylation
|
SLC12A2 |
0.542 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264625 |
|
|
|
|
| pmid |
sentence |
| 21613606 |
We have shown that with-no-lysine kinase 3 (WNK3) possesses several properties that suggest it could be the Cl−/volume-sensitive regulatory kinase that, in association with protein phosphatases, reciprocally modifies the phosphorylation/dephosphorylation states of the SLC12 proteins and thus their activities|WNK3 activates NKCC1/2 and NCC and inhibits the KCCs |
|
| Publications: |
1 |
3.0
SLC12A2
- 
- 