| + |
WNK3 | up-regulates activity 
phosphorylation
|
WNK1 |
0.287 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260789 |
Ser382 |
KRASFAKsVIGTPEF |
Homo sapiens |
|
| pmid |
sentence |
| 22032326 |
We found that wild-type WNK2 (Figure 8A) or WNK3 (Figure 8B) phosphorylated kinase-inactive WNK1 (1–667, D368A) at Ser382 in vitro. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
WNK3 | down-regulates activity 
phosphorylation
|
SLC12A6 |
0.457 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260911 |
Ser96 |
IEDLSQNsITGEHSQ |
Homo sapiens |
|
| pmid |
sentence |
| 24043619 |
WNK3, which inhibits the activity of KCC3, promoted phosphorylation of Ser-96 as well as Thr-991 and Thr-1048. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260910 |
Thr1048 |
YQEKVHMtWTKDKYM |
Homo sapiens |
|
| pmid |
sentence |
| 24043619 |
WNK3, which inhibits the activity of KCC3, promoted phosphorylation of Ser-96 as well as Thr-991 and Thr-1048. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260912 |
Thr991 |
SAYTYERtLMMEQRS |
Homo sapiens |
|
| pmid |
sentence |
| 24043619 |
WNK3, which inhibits the activity of KCC3, promoted phosphorylation of Ser-96 as well as Thr-991 and Thr-1048. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264629 |
|
|
|
|
| pmid |
sentence |
| 21613606 |
We have shown that with-no-lysine kinase 3 (WNK3) possesses several properties that suggest it could be the Cl−/volume-sensitive regulatory kinase that, in association with protein phosphatases, reciprocally modifies the phosphorylation/dephosphorylation states of the SLC12 proteins and thus their activities|WNK3 activates NKCC1/2 and NCC and inhibits the KCCs |
|
| Publications: |
4 |
Organism: |
Homo Sapiens, |
| + |
KLHL2 | down-regulates quantity by destabilization
binding
|
WNK3 |
0.49 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272121 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 23838290 |
We found that KLHL2, as well as KLHL3, was co-immunoprecipitated with all four WNK isoforms. The direct interaction of KLHL2 with WNKs was confirmed on fluorescence correlation spectroscopy. Co-expression of KLHL2 and Cullin3 decreased the abundance of WNK1, WNK3 and WNK4 within HEK293T cells, and a significant increase of WNK4 ubiquitination by KLHL2 and Cullin3 was observed both in HEK293T cells and in an in vitro ubiquitination assay. These results suggest that KLHL2-Cullin3 also functions as an E3-ligase for WNK isoforms within the body. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
WNK3 | up-regulates activity
phosphorylation
|
SLC12A3 |
0.468 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264624 |
|
|
|
|
| pmid |
sentence |
| 21613606 |
We have shown that with-no-lysine kinase 3 (WNK3) possesses several properties that suggest it could be the Cl−/volume-sensitive regulatory kinase that, in association with protein phosphatases, reciprocally modifies the phosphorylation/dephosphorylation states of the SLC12 proteins and thus their activities|WNK3 activates NKCC1/2 and NCC and inhibits the KCCs |
|
| Publications: |
1 |
| + |
WNK3 | down-regulates activity
phosphorylation
|
SLC12A4 |
0.478 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264627 |
|
|
|
|
| pmid |
sentence |
| 21613606 |
We have shown that with-no-lysine kinase 3 (WNK3) possesses several properties that suggest it could be the Cl−/volume-sensitive regulatory kinase that, in association with protein phosphatases, reciprocally modifies the phosphorylation/dephosphorylation states of the SLC12 proteins and thus their activities|WNK3 activates NKCC1/2 and NCC and inhibits the KCCs |
|
| Publications: |
1 |
| + |
Cullin 3-RBX1-Skp1 | down-regulates quantity by destabilization
polyubiquitination
|
WNK3 |
0.343 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272126 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 23838290 |
We found that KLHL2, as well as KLHL3, was co-immunoprecipitated with all four WNK isoforms. The direct interaction of KLHL2 with WNKs was confirmed on fluorescence correlation spectroscopy. Co-expression of KLHL2 and Cullin3 decreased the abundance of WNK1, WNK3 and WNK4 within HEK293T cells, and a significant increase of WNK4 ubiquitination by KLHL2 and Cullin3 was observed both in HEK293T cells and in an in vitro ubiquitination assay. These results suggest that KLHL2-Cullin3 also functions as an E3-ligase for WNK isoforms within the body. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
WNK3 | down-regulates activity
phosphorylation
|
SLC12A7 |
0.465 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264630 |
|
|
|
|
| pmid |
sentence |
| 21613606 |
We have shown that with-no-lysine kinase 3 (WNK3) possesses several properties that suggest it could be the Cl−/volume-sensitive regulatory kinase that, in association with protein phosphatases, reciprocally modifies the phosphorylation/dephosphorylation states of the SLC12 proteins and thus their activities|WNK3 activates NKCC1/2 and NCC and inhibits the KCCs |
|
| Publications: |
1 |
| + |
WNK3 | up-regulates activity
phosphorylation
|
SLC12A2 |
0.542 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264625 |
|
|
|
|
| pmid |
sentence |
| 21613606 |
We have shown that with-no-lysine kinase 3 (WNK3) possesses several properties that suggest it could be the Cl−/volume-sensitive regulatory kinase that, in association with protein phosphatases, reciprocally modifies the phosphorylation/dephosphorylation states of the SLC12 proteins and thus their activities|WNK3 activates NKCC1/2 and NCC and inhibits the KCCs |
|
| Publications: |
1 |
| + |
WNK3 | down-regulates activity
phosphorylation
|
SLC12A5 |
0.474 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264628 |
|
|
|
|
| pmid |
sentence |
| 21613606 |
We have shown that with-no-lysine kinase 3 (WNK3) possesses several properties that suggest it could be the Cl−/volume-sensitive regulatory kinase that, in association with protein phosphatases, reciprocally modifies the phosphorylation/dephosphorylation states of the SLC12 proteins and thus their activities|WNK3 activates NKCC1/2 and NCC and inhibits the KCCs |
|
| Publications: |
1 |
| + |
WNK3 | up-regulates activity
phosphorylation
|
SLC12A1 |
0.514 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264626 |
|
|
|
|
| pmid |
sentence |
| 21613606 |
We have shown that with-no-lysine kinase 3 (WNK3) possesses several properties that suggest it could be the Cl−/volume-sensitive regulatory kinase that, in association with protein phosphatases, reciprocally modifies the phosphorylation/dephosphorylation states of the SLC12 proteins and thus their activities|WNK3 activates NKCC1/2 and NCC and inhibits the KCCs |
|
| Publications: |
1 |
3.0
WNK3
- 
- 