| + |
MAPK1 |
phosphorylation
|
TNKS1BP1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262781 |
Thr1032 |
GGLFSPStAHVPDGA |
Mus musculus |
NIH-3T3 Cell |
| pmid |
sentence |
| 22028470 |
We have optimized a chemical genetic system using analog-sensitive ERK2, a form of ERK2 engineered to use an analog of adenosine 5'-triphosphate (ATP), to tag and isolate ERK2 substrates in vitro. This approach identified 80 proteins phosphorylated by ERK2, 13 of which are known ERK2 substrates. With this improved methodology, we detected 98 sites directly phosphorylated by ERK2 on 80 proteins from NIH 3T3-L1 fibroblasts. Thirteen of these proteins are known substrates and the rest represent previously unknown kinase/substrate interactions. (table1) |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262782 |
Thr131 |
KEEPPPLtPPARCAA |
Mus musculus |
NIH-3T3 Cell |
| pmid |
sentence |
| 22028470 |
We have optimized a chemical genetic system using analog-sensitive ERK2, a form of ERK2 engineered to use an analog of adenosine 5'-triphosphate (ATP), to tag and isolate ERK2 substrates in vitro. This approach identified 80 proteins phosphorylated by ERK2, 13 of which are known ERK2 substrates. With this improved methodology, we detected 98 sites directly phosphorylated by ERK2 on 80 proteins from NIH 3T3-L1 fibroblasts. Thirteen of these proteins are known substrates and the rest represent previously unknown kinase/substrate interactions. (table1) |
|
| Publications: |
2 |
Organism: |
Mus Musculus |
| + |
TNKS1BP1 | form complex 
binding
|
CCR4-NOT complex |
0.623 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-268304 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19558367 |
In the present study, we examine the composition of the human Ccr4-Not complex in an in-depth proteomic approach using stable cell lines expressing tagged CNOT proteins. We find at least four different variants of the human complex, consisting of seven stable core proteins and mutually exclusive associated mRNA deadenylase subunits. Interestingly, human CNOT4 is in a separate approximately 200 kDa complex. Furthermore, analyses of associated proteins indicate involvement of Ccr4-Not complexes in splicing, transport and localization of RNA molecules. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
TNKS1BP1
- 
- 