| + |
SMAD5/SMAD4 | up-regulates 
transcriptional regulation
|
DLX5 |
0.328 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-255790 |
|
|
Mus musculus |
C2C12 Cell |
| pmid |
sentence |
| 12815054 |
Over-expression of Smad1 or Smad5, mediators of BMP-signaling, also induced Dlx5 expression even in the absence of BMP-2 treatment concomitant with positive ALP staining |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
BMPR1A | up-regulates activity 
phosphorylation
|
SMAD5/SMAD4 |
0.68 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-255779 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 9442019 |
In this study, we isolated human Smad5 and found that Smad5 was involved in BMP-2 signaling cascades, which mediate the bone-inducing effects of BMP-2. Smad5 was directly serine-phosphorylated by BMPIR through a physical interaction. The activated Smad5 subsequently formed a complex with DPC4, and this complex was then translocated to the nucleus. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-255788 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 19620713 |
The expression of CA-BMPr1A and CA-BMPr1B mRNA was confirmed by RT-PCR using appropriate primers to distinguish expression of the constitutively active receptors from endogenous BMP receptors; specific antibodies for these receptors were not available. However, the functional effects of their expression, i.e., phosphorylation of Smad1/5/8 and p38 MAPK, verify overexpression of the constitutively active receptors (Fig. 3B). Thus, their overexpression provoked a substantial rise in the phosphorylation of Smad1/5/8 and p38 MAPK, known downstream phosphorylated intermediates in the BMP signaling pathway (Fig. 3B) (16, 17). |
|
| Publications: |
2 |
Organism: |
Homo Sapiens, Mus Musculus |
| + |
SMAD5 | form complex 
binding
|
SMAD5/SMAD4 |
0.652 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-255266 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20957627 |
Whereas alk5 signalling is mediated by phosphorylation of smad2 and smad3 proteins, alk1 signalling is mediated by smad1, smad5, and smad8. Activated smads form a complex with the common smad (co-smad; smad4 in mammals) and shuttle into the nucleus. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-255775 |
|
|
Homo sapiens |
C2C12 Cell, HEK-293-EBNA Cell, L-6 Myoblast Cell |
| pmid |
sentence |
| 9442019 |
These data suggest that activation of Smad5 and subsequent Smad5-DPC4 complex formation are key steps in the BMP signaling pathway, which mediates BMP-2-induced osteoblastic differentiation of the C2C12 mesenchymal cells. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
SMAD5/SMAD4 | up-regulates
transcriptional regulation
|
RUNX2 |
0.586 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-255783 |
|
|
Mus musculus |
C2C12 Cell |
| pmid |
sentence |
| 11073979 |
As shown in Fig. 8A, overexpression of Smad5 by itself induced Runx2 expression even in the absence of BMP-2 (lane 5). Western blot analysis also confirmed the induced level of Runx2 protein in C2C12-Sm5 cells (Fig. 8B) |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
SMAD4 | form complex
binding
|
SMAD5/SMAD4 |
0.652 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-255267 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20957627 |
Whereas alk5 signalling is mediated by phosphorylation of smad2 and smad3 proteins, alk1 signalling is mediated by smad1, smad5, and smad8. Activated smads form a complex with the common smad (co-smad; smad4 in mammals) and shuttle into the nucleus. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
SMAD5/SMAD4