| + |
HPS1 | form complex 
binding
|
BLOC-3 |
0.743 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260691 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20048159 |
Two of these genes, HPS1 and HPS4, encode components of the biogenesis of lysosome-related organelles complex-3 (BLOC-3). Herein we show that recombinant HPS1-HPS4 produced in insect cells can be efficiently isolated as a 1:1 heterodimer. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
BLOC-3 | up-regulates activity 
guanine nucleotide exchange factor
|
RAB38 |
0.55 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260694 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23174301 |
HPS1/HPS4 was active exclusively for RAB32 and RAB38. Moreover, when overexpressed with HPS1 in HeLa cells, a mitochondrially restricted form of HPS4 preferentially recruited GFP-tagged RAB32 or RAB38 – but not the related RAB7 or RAB9 – to mitochondria. Activity in both assays required both HPS1 and HPS4. Finally, depletion of HPS1 or HPS4 by siRNA in a pigmented human melanoma cell line, MNT-1, resulted in the mislocalization of RAB32. These data provide strong evidence that BLOC-3 is a selective GEF for RAB32 and RAB38 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
HPS4 | form complex
binding
|
BLOC-3 |
0.743 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260692 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20048159 |
Two of these genes, HPS1 and HPS4, encode components of the biogenesis of lysosome-related organelles complex-3 (BLOC-3). Herein we show that recombinant HPS1-HPS4 produced in insect cells can be efficiently isolated as a 1:1 heterodimer. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
BLOC-3 | up-regulates activity
guanine nucleotide exchange factor
|
RAB32 |
0.546 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260693 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23174301 |
HPS1/HPS4 was active exclusively for RAB32 and RAB38. Moreover, when overexpressed with HPS1 in HeLa cells, a mitochondrially restricted form of HPS4 preferentially recruited GFP-tagged RAB32 or RAB38 – but not the related RAB7 or RAB9 – to mitochondria. Activity in both assays required both HPS1 and HPS4. Finally, depletion of HPS1 or HPS4 by siRNA in a pigmented human melanoma cell line, MNT-1, resulted in the mislocalization of RAB32. These data provide strong evidence that BLOC-3 is a selective GEF for RAB32 and RAB38 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
BLOC-3
CPX-5043