| + |
PKA | down-regulates activity 
phosphorylation
|
GAD1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276009 |
Thr91 |
RDARFRRtETDFSNL |
Homo sapiens |
Brain |
| pmid |
sentence |
| 15147202 |
Here, we report the effect of phosphorylation on the two well-defined GAD isoforms, namely, GAD65 and GAD67, using highly purified preparations of recombinant human brain GAD65 and GAD67. GAD65 was activated by phosphorylation, while GAD67 was inhibited by phosphorylation.We further demonstrate that protein kinase A (PKA) and protein kinase C isoform epsilon are the protein kinases responsible for phosphorylation and regulation of GAD67 and GAD65, respectively. We have identified one specific phosphorylation site, threonine 91 (T91), in hGAD67 that can be phosphorylated by PKA using MALDI-TOF. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PRKCE | down-regulates activity
phosphorylation
|
GAD1 |
0.326 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-249264 |
Thr91 |
RDARFRRtETDFSNL |
in vitro |
|
| pmid |
sentence |
| 15147202 |
We have identified one specific phosphorylation site, threonine 91 (T91), in hGAD67 that can be phosphorylated by PKA using MALDI-TOF. Site-directed mutation of T91 to alanine abolished PKA-mediated phosphorylation and inhibition of GAD activity. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
GAD1 | down-regulates quantity
chemical modification
|
L-glutamate(1-) |
0.8 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-267551 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 32041144 |
Glutamate decarboxylase (GAD; EC 4.1.1.15) is a unique pyridoxal 5-phosphate (PLP)-dependent enzyme that specifically catalyzes the decarboxylation of L-glutamic acid to produce γ-aminobutyric acid (GABA), which exhibits several well-known physiological functions. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Aspartate and asparagine metabolism |
| + |
DNMT1 | down-regulates quantity by repression 
transcriptional regulation
|
GAD1 |
0.356 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254574 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19029285 |
induction of the reelin and GAD67 mRNAs is accompanied by the dissociation of repressor complexes containing all three DNMTs, MeCP2, and HDAC1 from the corresponding promoters and by increased local histone acetylation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Rett syndrome |
| + |
HDAC1 | down-regulates quantity by repression
transcriptional regulation
|
GAD1 |
0.287 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254576 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19029285 |
induction of the reelin and GAD67 mRNAs is accompanied by the dissociation of repressor complexes containing all three DNMTs, MeCP2, and HDAC1 from the corresponding promoters and by increased local histone acetylation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MECP2 | down-regulates quantity by repression
transcriptional regulation
|
GAD1 |
0.379 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254579 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19029285 |
induction of the reelin and GAD67 mRNAs is accompanied by the dissociation of repressor complexes containing all three DNMTs, MeCP2, and HDAC1 from the corresponding promoters and by increased local histone acetylation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Rett syndrome |
| + |
GAD1 | up-regulates quantity 
chemical modification
|
gamma-aminobutyric acid |
0.8 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-267552 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 32041144 |
Glutamate decarboxylase (GAD; EC 4.1.1.15) is a unique pyridoxal 5-phosphate (PLP)-dependent enzyme that specifically catalyzes the decarboxylation of L-glutamic acid to produce γ-aminobutyric acid (GABA), which exhibits several well-known physiological functions. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Aspartate and asparagine metabolism |
3.0
GAD1
- 
- 