| + |
CHEK1 | down-regulates quantity by destabilization 
phosphorylation
|
PPP1R12A |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277870 |
Ser20 |
QLKRWIGsETDLEPP |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 29262732 |
MYPT1 is phosphorylated by CDK1, thus recruiting protein phosphatase 1β (PP1cβ) to dephosphorylate and inactivate Plk1. Here we identified that Chk1 directly interacts with MYPT1 and preferentially phosphorylates MYPT1 at Ser20, which is essential for MYPT1-PP1cβ interaction and subsequent Plk1 dephosphorylation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
LATS1 | up-regulates activity 
phosphorylation
|
PPP1R12A |
0.461 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278184 |
Ser445 |
LGLRKTGsYGALAEI |
Homo sapiens |
|
| pmid |
sentence |
| 22641346 |
LATS1 directly and preferentially phosphorylated serine 445 (S445) of MYPT1.|This suggests that LATS1 promotes MYPT1 to antagonize PLK1 activity. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278185 |
Ser473 |
GVTRSASsPRLSSSL |
Homo sapiens |
|
| pmid |
sentence |
| 22641346 |
The present study identified S473 as a one of the significant LATS1 phosphorylation sites within MYPT1 (Fig. 3, B and E).|This suggests that LATS1 promotes MYPT1 to antagonize PLK1 activity. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
NUAK1 | down-regulates
phosphorylation
|
PPP1R12A |
0.496 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-164747 |
Ser445 |
LGLRKTGsYGALAEI |
Homo sapiens |
|
| pmid |
sentence |
| 20354225 |
Phosphorylation of ser(445), ser(472), and ser(910) of mypt1 by nuak1 promoted the interaction of mypt1 with 14-3-3 adaptor proteins, thereby suppressing phosphatase activity. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-164751 |
Ser472 |
AGVTRSAsSPRLSSS |
Homo sapiens |
|
| pmid |
sentence |
| 20354225 |
Phosphorylation of ser(445), ser(472), and ser(910) of mypt1 by nuak1 promoted the interaction of mypt1 with 14-3-3 adaptor proteins, thereby suppressing phosphatase activity. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-22572 |
Ser910 |
SLLGRSGsYSYLEER |
Homo sapiens |
|
| pmid |
sentence |
| 20354225 |
Phosphorylation of ser(445), ser(472), and ser(910) of mypt1 by nuak1 promoted the interaction of mypt1 with 14-3-3 adaptor proteins, thereby suppressing phosphatase activity. |
|
| Publications: |
3 |
Organism: |
Homo Sapiens |
| + |
ILK | down-regulates activity
phosphorylation
|
PPP1R12A |
0.6 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262884 |
Thr500 |
RLAYVAPtIPRRLAS |
in vitro |
|
| pmid |
sentence |
| 12030846 |
MYPT1 was phosphorylated by ILK and phosphorylation sites in the N- and C-terminal fragments of MYPT1 were detected. From sequence analyses, three sites were identified: a primary site at Thr(709), and two other sites at Thr(695) and Thr(495). ILK produced an intermediate level of inhibition |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262886 |
Thr696 |
ARQSRRStQGVTLTD |
in vitro |
|
| pmid |
sentence |
| 12030846 |
MYPT1 was phosphorylated by ILK and phosphorylation sites in the N- and C-terminal fragments of MYPT1 were detected. From sequence analyses, three sites were identified: a primary site at Thr(709), and two other sites at Thr(695) and Thr(495). ILK produced an intermediate level of inhibition |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262885 |
Thr710 |
DLQEAEKtIGRSRST |
in vitro |
|
| pmid |
sentence |
| 12030846 |
MYPT1 was phosphorylated by ILK and phosphorylation sites in the N- and C-terminal fragments of MYPT1 were detected. From sequence analyses, three sites were identified: a primary site at Thr(709), and two other sites at Thr(695) and Thr(495). ILK produced an intermediate level of inhibition |
|
| Publications: |
3 |
Organism: |
In Vitro |
| + |
ROCK1 | down-regulates
phosphorylation
|
PPP1R12A |
0.785 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-42354 |
Thr696 |
ARQSRRStQGVTLTD |
Homo sapiens |
|
| pmid |
sentence |
| 8662509 |
Rho-associated kinase (rho-kinase) phosphorylated mbs and consequently inactivated myosin phosphatase. Rho appears to inhibit myosin phosphatase through the action of rho-kinase. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ILK | down-regulates
phosphorylation
|
PPP1R12A |
0.6 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-87924 |
Thr696 |
ARQSRRStQGVTLTD |
Homo sapiens |
|
| pmid |
sentence |
| 12030846 |
Mypt1 was phosphorylated by ilk and phosphorylation sites in the n- and c-terminal fragments of mypt1 were detected. From sequence analyses, three sites were identified: a primary site at thr(709), and two other sites at thr(695) and thr(495) |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-87928 |
Thr710 |
DLQEAEKtIGRSRST |
Homo sapiens |
|
| pmid |
sentence |
| 12030846 |
Mypt1 was phosphorylated by ilk and phosphorylation sites in the n- and c-terminal fragments of mypt1 were detected. From sequence analyses, three sites were identified: a primary site at thr(709), and two other sites at thr(695) and thr(495). phosphorylation of the various sites indicated that thr695 was the major inhibitory site, thr709 had only a slight inhibitory effect |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
ROCK2 | down-regulates activity
phosphorylation
|
PPP1R12A |
0.796 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-249164 |
Thr853 |
PREKRRStGVSFWTQ |
in vitro |
|
| pmid |
sentence |
| 12220642 |
Rho kinase is known to control smooth muscle contractility by phosphorylating the 110 kDa myosin-targetting subunit (MYPT1) of the myosin-associated form of protein phosphatase 1 (PP1M). Phosphorylation of MYPT1 at Thr695 has previously been reported to inhibit the catalytic activity of PP1. Here, we show that the phosphorylation of Thr850 by Rho kinase dissociates PP1M from myosin, providing a second mechanism by which myosin phosphatase activity is inhibited. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
ROCK1 | down-regulates activity
phosphorylation
|
PPP1R12A |
0.785 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-249034 |
Thr853 |
PREKRRStGVSFWTQ |
Mus musculus |
|
| pmid |
sentence |
| 10601309 |
Phosphorylation by Rho-kinase inhibited MP activity and this reflected a decrease in V(max). Activity of MP with different substrates also was inhibited by phosphorylation. Two major sites of phosphorylation on MYPT1 were Thr(695) and Thr(850). |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
NUAK2 | down-regulates activity
phosphorylation
|
PPP1R12A |
0.458 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279081 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 30622137 |
NUAK2 phosphorylates and inhibits MYPT1, the regulatory subunit of MLC phosphatase, stabilizing actin filaments and mediating contraction of smooth muscle cells ( xref ). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RAF1 | down-regulates activity
phosphorylation
|
PPP1R12A |
0.271 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278979 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22225925 |
In hESC, Raf-1 directly phosphorylates and inactivates MYPT1, and this process requires kinase active Raf-1 protein. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DAPK3 | down-regulates activity
phosphorylation
|
PPP1R12A |
0.536 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279030 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20130087 |
We conclude from our results Par-4 operates through a "padlock" model in which binding of Par-4 to MYPT1 activates MP by blocking access to the inhibitory phosphorylation sites, and inhibitory phosphorylation of MYPT1 by ZIPK requires "unlocking" of Par-4 by phosphorylation and displacement of Par-4 from the MP complex.|We have also demonstrated that Par-4 is required for agonist induced, ZIPK mediated inhibition of MYPT1 and thus is an important amplifier of inputs to MP. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
4.0
PPP1R12A
- 
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