| + |
PHF2 | down-regulates activity 
demethylation
|
H3-3A |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264519 |
Lys10 |
RTKQTARkSTGGKAP |
Homo sapiens |
|
| pmid |
sentence |
| 21532585 |
PHF2, a jmjC demethylase, is enzymatically inactive by itself, but becomes an active H3K9Me2 demethylase through PKA-mediated phosphorylation. This modification leads to targeting of the PHF2–ARID5B complex to its target promoters, where it removes the repressive H3K9Me2 mark. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PHF2 | down-regulates activity
demethylation
|
H3-4 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264520 |
Lys10 |
RTKQTARkSTGGKAP |
Homo sapiens |
|
| pmid |
sentence |
| 21532585 |
PHF2, a jmjC demethylase, is enzymatically inactive by itself, but becomes an active H3K9Me2 demethylase through PKA-mediated phosphorylation. This modification leads to targeting of the PHF2–ARID5B complex to its target promoters, where it removes the repressive H3K9Me2 mark. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PHF2 | down-regulates activity
demethylation
|
H3C1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264521 |
Lys10 |
RTKQTARkSTGGKAP |
Homo sapiens |
|
| pmid |
sentence |
| 21532585 |
PHF2, a jmjC demethylase, is enzymatically inactive by itself, but becomes an active H3K9Me2 demethylase through PKA-mediated phosphorylation. This modification leads to targeting of the PHF2–ARID5B complex to its target promoters, where it removes the repressive H3K9Me2 mark. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PHF2 | up-regulates activity 
demethylation
|
H3-4 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264517 |
Lys5 |
kQTARKST |
Homo sapiens |
|
| pmid |
sentence |
| 21532585 |
PHF2, a jmjC demethylase, is enzymatically inactive by itself, but becomes an active H3K9Me2 demethylase through PKA-mediated phosphorylation. This modification leads to targeting of the PHF2–ARID5B complex to its target promoters, where it removes the repressive H3K9Me2 mark. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PHF2 | up-regulates activity
demethylation
|
H3-3A |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264518 |
Lys5 |
kQTARKST |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 21532585 |
PHF2, a jmjC demethylase, is enzymatically inactive by itself, but becomes an active H3K9Me2 demethylase through PKA-mediated phosphorylation. This modification leads to targeting of the PHF2–ARID5B complex to its target promoters, where it removes the repressive H3K9Me2 mark. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PHF2 | up-regulates activity
demethylation
|
H3C1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264516 |
Lys5 |
kQTARKST |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 21532585 |
PHF2, a jmjC demethylase, is enzymatically inactive by itself, but becomes an active H3K9Me2 demethylase through PKA-mediated phosphorylation. This modification leads to targeting of the PHF2–ARID5B complex to its target promoters, where it removes the repressive H3K9Me2 mark. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PKA | up-regulates activity
phosphorylation
|
PHF2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264513 |
Ser1056 |
FLTQRRPsASSPNNN |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 21532585 |
PHF2, a jmjC demethylase, is enzymatically inactive by itself, but becomes an active H3K9Me2 demethylase through PKA-mediated phosphorylation. We found that phosphorylated PHF2 then associates with ARID5B, a DNA-binding protein, and induce demethylation of methylated ARID5B. This modification leads to targeting of the PHF2-ARID5B complex to its target promoters, where it removes the repressive H3K9Me2 mark. Replacement of all of four serine residues by alanines (4SA: Ser 757/Ser 899/Ser 954/Ser 1056) fully abrogated PKA phosphorylation of PHF2 (Fig. 2h). |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264510 |
Ser757 |
NARVKKEsGSSAAGI |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 21532585 |
PHF2, a jmjC demethylase, is enzymatically inactive by itself, but becomes an active H3K9Me2 demethylase through PKA-mediated phosphorylation. We found that phosphorylated PHF2 then associates with ARID5B, a DNA-binding protein, and induce demethylation of methylated ARID5B. This modification leads to targeting of the PHF2-ARID5B complex to its target promoters, where it removes the repressive H3K9Me2 mark. Replacement of all of four serine residues by alanines (4SA: Ser 757/Ser 899/Ser 954/Ser 1056) fully abrogated PKA phosphorylation of PHF2 (Fig. 2h). |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264511 |
Ser899 |
RSKKRKGsDDAPYSP |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 21532585 |
PHF2, a jmjC demethylase, is enzymatically inactive by itself, but becomes an active H3K9Me2 demethylase through PKA-mediated phosphorylation. We found that phosphorylated PHF2 then associates with ARID5B, a DNA-binding protein, and induce demethylation of methylated ARID5B. This modification leads to targeting of the PHF2-ARID5B complex to its target promoters, where it removes the repressive H3K9Me2 mark. Replacement of all of four serine residues by alanines (4SA: Ser 757/Ser 899/Ser 954/Ser 1056) fully abrogated PKA phosphorylation of PHF2 (Fig. 2h). |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264512 |
Ser954 |
QKSKKKKsAKRKLTP |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 21532585 |
PHF2, a jmjC demethylase, is enzymatically inactive by itself, but becomes an active H3K9Me2 demethylase through PKA-mediated phosphorylation. We found that phosphorylated PHF2 then associates with ARID5B, a DNA-binding protein, and induce demethylation of methylated ARID5B. This modification leads to targeting of the PHF2-ARID5B complex to its target promoters, where it removes the repressive H3K9Me2 mark. Replacement of all of four serine residues by alanines (4SA: Ser 757/Ser 899/Ser 954/Ser 1056) fully abrogated PKA phosphorylation of PHF2 (Fig. 2h). |
|
| Publications: |
4 |
Organism: |
Homo Sapiens |
| + |
2-oxoglutarate(2-) | up-regulates activity
chemical activation
|
PHF2 |
0.8 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-273464 |
|
|
|
|
| pmid |
sentence |
| 29981745 |
Histone lysine demethylases (KDMs) are 2-oxoglutarate-dependent dioxygenases (2-OGDDs) that regulate gene expression by altering chromatin structure. |2-OG is a central intermediate of the Krebs cycle, where it is produced by isocitrate dehydrogenase (IDH) isoenzymes 2 and 3. |
|
| Publications: |
1 |
| + |
PHF2 | down-regulates activity
demethylation
|
Histone H3 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-265343 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21532585 |
PHF2, a jmjC demethylase, is enzymatically inactive by itself, but becomes an active H3K9Me2 demethylase through PKA-mediated phosphorylation. This modification leads to targeting of the PHF2–ARID5B complex to its target promoters, where it removes the repressive H3K9Me2 mark. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PHF2 | up-regulates activity
binding
|
ARID5B |
0.542 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264514 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 21532585 |
We found that phosphorylated PHF2 then associates with ARID5B, a DNA-binding protein, and induce demethylation of methylated ARID5B. Assembly of the PHF2–ARID5B complex, its recruitment to target promoters, and its H3H9Me2 demethylase activity were dependent on PKA activity. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ARID5B | up-regulates activity
binding
|
PHF2 |
0.542 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264515 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 21532585 |
We found that phosphorylated PHF2 then associates with ARID5B, a DNA-binding protein, and induce demethylation of methylated ARID5B. Assembly of the PHF2–ARID5B complex, its recruitment to target promoters, and its H3H9Me2 demethylase activity were dependent on PKA activity. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
PHF2
- 
- 