| + |
HNF1A | up-regulates quantity by expression 
transcriptional regulation
|
AFP |
0.329 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254447 |
|
|
Homo sapiens |
HEP-3B Cell |
| pmid |
sentence |
| 9792724 |
AFP promoter-chloramphenicol acetyltransferase transient transfection assays demonstrated that the level of HNF1 had a direct impact on basal transcription as well as RA-mediated down-regulation of the AFP gene, and that co-transfection of HNF1 and HNF4, but not transfection of either factor alone, reversed the RA-mediated inhibition. Taken together these data point to an interaction among the RA, HNF1, and HNF4 signals, which is reflected in decreased expression of AFP. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254435 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 11314020 |
We investigated AFP gene regulation in AFP-GC by an active transcription factor, HNF1 (hepatocyte nuclear factor 1) and a repressive transcription factor, ATBF1 (AT motif binding factor 1). CAT assays showed the direct inhibition of AFP gene expression by ATBF1. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254637 |
|
|
Homo sapiens |
Hep-G2 Cell |
| pmid |
sentence |
| 7549116 |
HNF-1 beta was found to be more potent than HNF-1 alpha in activating the AFP promoter in the HepG2 cells. |
|
| Publications: |
3 |
Organism: |
Homo Sapiens |
| + |
ZFHX3 | down-regulates quantity by repression 
transcriptional regulation
|
AFP |
0.415 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-255625 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 14654895 |
these results corroborated the previous reports that ATBF1 regulated AFP expression and inhibited transcription. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254436 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 11314020 |
We investigated AFP gene regulation in AFP-GC by an active transcription factor, HNF1 (hepatocyte nuclear factor 1) and a repressive transcription factor, ATBF1 (AT motif binding factor 1). CAT assays showed the direct inhibition of AFP gene expression by ATBF1. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
SIRT2 | up-regulates quantity by expression
transcriptional regulation
|
AFP |
0.256 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254487 |
|
|
Homo sapiens |
Hep-G2 Cell |
| pmid |
sentence |
| 14522900 |
In this study, we found that a subset of ING family members strongly repressed human alpha-fetoprotein (AFP) promoter activity but stimulated the p21(WAF1) promoter in parallel experiments in the same cell type, similar to the effects of p53. Both ING1 and p53 were able to suppress AFP transcription and cause p21 induction; hSIR2, a negative regulator of the p53 protein, showed the opposite effects on the AFP promoter and, like HDAC1, repressed p21 promoter activity. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NF1 | down-regulates quantity by repression
transcriptional regulation
|
AFP |
0.271 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254636 |
|
|
Homo sapiens |
Hep-G2 Cell |
| pmid |
sentence |
| 7549116 |
Our results pointed to a key role that NF1 might play in the functioning of the AFP promoter. Indeed, overexpression of NF1 induced a specific decrease in the activity of the AFP promoter. Competition between NF1 and HNF-1 for binding to their overlapping binding sites on the AFP promoter would be critical for modulating its activity. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
HNF4G | up-regulates quantity by expression
transcriptional regulation
|
AFP |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254442 |
|
|
Homo sapiens |
HEP-3B Cell |
| pmid |
sentence |
| 9792724 |
AFP promoter-chloramphenicol acetyltransferase transient transfection assays demonstrated that the level of HNF1 had a direct impact on basal transcription as well as RA-mediated down-regulation of the AFP gene, and that co-transfection of HNF1 and HNF4, but not transfection of either factor alone, reversed the RA-mediated inhibition. Taken together these data point to an interaction among the RA, HNF1, and HNF4 signals, which is reflected in decreased expression of AFP. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ZBTB20 | down-regulates quantity by repression 
transcriptional regulation
|
AFP |
0.439 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-266867 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 23776228 |
Zinc finger and BTB domain-containing 20 (ZBTB20), a member of BTB/POZ family, functions in neurogenesis and represses α-fetoprotein gene transcription in liver. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
HNF1B | up-regulates quantity by expression
transcriptional regulation
|
AFP |
0.317 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254638 |
|
|
Homo sapiens |
Hep-G2 Cell |
| pmid |
sentence |
| 7549116 |
HNF-1 beta was found to be more potent than HNF-1 alpha in activating the AFP promoter in the HepG2 cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NANOG | down-regulates quantity by repression
transcriptional regulation
|
AFP |
0.439 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254621 |
|
|
Homo sapiens |
Embryonic Stem Cell, Embryonic Carcinoma Cell |
| pmid |
sentence |
| 15983365 |
Transfection of NANOG-specific small interfering RNAs reduced levels of NANOG transcript and protein and induced activation of the extraembryonic endoderm-associated genes GATA4, GATA6, LAMININ B1, and AFP as well as upregulation of trophectoderm-associated genes CDX2, GATA2, hCG-alpha, and hCG-beta. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TP53 | down-regulates quantity by repression
transcriptional regulation
|
AFP |
0.436 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254482 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 14522900 |
In this study, we found that a subset of ING family members strongly repressed human alpha-fetoprotein (AFP) promoter activity but stimulated the p21(WAF1) promoter in parallel experiments in the same cell type, similar to the effects of p53. Both ING1 and p53 were able to suppress AFP transcription and cause p21 induction |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ING1 | down-regulates quantity by repression
transcriptional regulation
|
AFP |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254480 |
|
|
Homo sapiens |
Hep-G2 Cell |
| pmid |
sentence |
| 14522900 |
In this study, we found that a subset of ING family members strongly repressed human alpha-fetoprotein (AFP) promoter activity but stimulated the p21(WAF1) promoter in parallel experiments in the same cell type, similar to the effects of p53. Both ING1 and p53 were able to suppress AFP transcription and cause p21 induction; hSIR2, a negative regulator of the p53 protein, showed the opposite effects on the AFP promoter and, like HDAC1, repressed p21 promoter activity. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
HNF4A | up-regulates quantity by expression
transcriptional regulation
|
AFP |
0.403 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254446 |
|
|
Homo sapiens |
HEP-3B Cell |
| pmid |
sentence |
| 9792724 |
AFP promoter-chloramphenicol acetyltransferase transient transfection assays demonstrated that the level of HNF1 had a direct impact on basal transcription as well as RA-mediated down-regulation of the AFP gene, and that co-transfection of HNF1 and HNF4, but not transfection of either factor alone, reversed the RA-mediated inhibition. Taken together these data point to an interaction among the RA, HNF1, and HNF4 signals, which is reflected in decreased expression of AFP. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ING2 | down-regulates quantity by repression
transcriptional regulation
|
AFP |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254485 |
|
|
Homo sapiens |
Hep-G2 Cell |
| pmid |
sentence |
| 14522900 |
ING1b and ING2 also repressed the AFP promoter in Hep3B p53-null cell lines, and p53 coexpression enhanced this transcriptional repression. Suppression of AFP gene transcription by ING was strongly dependent on AT-motifs that bind to the hepatocyte nuclear factor 1 (HNF1) transcription factor. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
all-trans-retinoic acid | down-regulates quantity by repression
|
AFP |
0.8 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254443 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 9792724 |
In this report, we show a distinctive effect of all-trans-retinoic acid (RA) in Hep3B cells. RA caused a marked decrease in AFP transcripts. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
AFP
- 
- 