| + |
MAPK3 | down-regulates 
phosphorylation
|
NANOG |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278487 |
Ser52 |
MPHTETVsPLPSSMD |
Homo sapiens |
|
| pmid |
sentence |
| 24793005 |
We found that activation of ERK1 signaling inhibited transactivation activity of Nanog.|We showed the direct phosphorylation of Nanog by ERK1 and clearly showed that Ser52 is the major phosphorylation site and Ser65 is weakly phosphorylated by ERK1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PRKCE | up-regulates activity 
phosphorylation
|
NANOG |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278203 |
Thr200 |
LPMWSNQtWNNSTWS |
Homo sapiens |
|
| pmid |
sentence |
| 23708658 |
Taken together, our results demonstrate that PKC\u03b5-mediated phosphorylation at T200 and T280 enhances Nanog protein stability in head and neck squamous cell carcinoma.|These observations confirm that PKCepsilon modulates Nanog transcriptional activity and demonstrate that Nanog is phosphorylated by PKCepsilon at T200 and T280 in vivo. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278202 |
Thr280 |
GLNVIQQtTRYFSTP |
Homo sapiens |
|
| pmid |
sentence |
| 23708658 |
PKCepsilon phosphorylates Nanog at T200 and T280 to enhance Nanog stability, homodimerization and Bmi1 promoter occupancy.|Taken together, our results demonstrate that PKC\u03b5-mediated phosphorylation at T200 and T280 enhances Nanog protein stability in head and neck squamous cell carcinoma. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
PTK2 | up-regulates activity
phosphorylation
|
NANOG |
0.278 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276410 |
Tyr174 |
QKASAPTyPSLYSSY |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 22493428 |
In addition, FAK directly phosphorylates Nanog in a dose-dependent manner by in vitro kinase assay and in cancer cells in vivo. The site-directed mutagenesis of Nanog tyrosines, Y35F and Y174F, blocked phosphorylation and binding by FAK. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276409 |
Tyr35 |
ICGPEENyPSLQMSS |
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 22493428 |
In addition, FAK directly phosphorylates Nanog in a dose-dependent manner by in vitro kinase assay and in cancer cells in vivo. The site-directed mutagenesis of Nanog tyrosines, Y35F and Y174F, blocked phosphorylation and binding by FAK. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
Av/b1 integrin | up-regulates quantity by expression 
|
NANOG |
0.327 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253276 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 18757303 |
Recombinant human LN-511 alone was suffi- cient to enable self-renewal of mouse ES cells for up to 169 days (31 passages). Cells cultured on LN-511 maintained expression of pluripotency markers, such as Oct4, Sox2, Tert, UTF1, and Nanog|ES cells interacted with LN-511 via 1-integrins, mostly a6b1 and aVb1. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
NANOG | up-regulates
|
Pluripotency |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-242076 |
|
|
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 16153702 |
Our results suggest that OCT4, SOX2, and NANOG contribute to pluripotency and self-renewal by activating their own genes and genes encoding components of key signaling pathways and by repressing genes that are key to developmental processes. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-242073 |
|
|
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 25126380 |
Sex determining region Y-box 2 (Sox2), a member of the SoxB1 transcription factor family, is an important transcriptional regulator in pluripotent stem cells (PSCs). Together with octamer-binding transcription factor 4 and Nanog, they co-operatively control gene expression in PSCs and maintain their pluripotency. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
NANOG | down-regulates quantity by repression 
transcriptional regulation
|
CGB Family |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254624 |
|
|
Homo sapiens |
Embryonic Stem Cell, Embryonic Carcinoma Cell |
| pmid |
sentence |
| 15983365 |
Transfection of NANOG-specific small interfering RNAs reduced levels of NANOG transcript and protein and induced activation of the extraembryonic endoderm-associated genes GATA4, GATA6, LAMININ B1, and AFP as well as upregulation of trophectoderm-associated genes CDX2, GATA2, hCG-alpha, and hCG-beta. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
KDM5B | down-regulates quantity by repression
transcriptional regulation
|
NANOG |
0.313 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-273451 |
|
|
Homo sapiens |
MDA-MB-231 Cell |
| pmid |
sentence |
| 31776402 |
Phosphorylation of KDM5B at Ser1456 attenuated the occupancy of KDM5B on the promoters of pluripotency genes. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NANOG | down-regulates quantity by repression
transcriptional regulation
|
CGA |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254623 |
|
|
Homo sapiens |
Embryonic Stem Cell, Embryonic Carcinoma Cell |
| pmid |
sentence |
| 15983365 |
Transfection of NANOG-specific small interfering RNAs reduced levels of NANOG transcript and protein and induced activation of the extraembryonic endoderm-associated genes GATA4, GATA6, LAMININ B1, and AFP as well as upregulation of trophectoderm-associated genes CDX2, GATA2, hCG-alpha, and hCG-beta. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NANOG | down-regulates quantity by repression
transcriptional regulation
|
FOXA2 |
0.464 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253166 |
|
|
Homo sapiens |
Mesenchymal Stem Cell |
| pmid |
sentence |
| 22795133 |
Knockdown of Oct4 or Nanog induced an increase in the expression of Pax6, Gata4, Gata6, Sox17, and FoxA2 in E, H, and p21KD MSCs ( Figure 3F and Figure S2D) |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMAD3/SMAD4 | up-regulates quantity by expression
transcriptional regulation
|
NANOG |
0.434 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-242052 |
|
|
Homo sapiens |
Esophageal Squamous Cell Carcinoma Cell |
| pmid |
sentence |
| 18682241 |
We also find that SMADs bind with the NANOG promoter and that SMAD2/3 activity enhances NANOG promoter activity. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-242055 |
|
|
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 19279133 |
Here, we show that Smad2/3, the downstream effectors of Activin/Nodal signalling, bind and directly control the activity of the Nanog gene in hESCs. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
NANOG | up-regulates quantity by expression
transcriptional regulation
|
DNMT1 |
0.444 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253157 |
|
|
Homo sapiens |
Mesenchymal Stem Cell |
| pmid |
sentence |
| 22795133 |
Oct4 and Nanog upregulate Dnmt1 through direct binding to its promoter, thereby leading to the repressed expression of p16 and p21 and genes associated with development and lineage differentiation |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ZMYM2 | down-regulates activity
binding
|
NANOG |
0.303 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269802 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 31363497 |
In this work, we identified ZMYM2/ZFP198, which physically associates with NANOG as a key negative regulator of NANOG-mediated reprogramming of both epiblast stem cells and somatic cells. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| Tissue: |
Epiblast |
| + |
NANOG | down-regulates quantity by repression
transcriptional regulation
|
GATA6 |
0.471 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253160 |
|
|
Homo sapiens |
Mesenchymal Stem Cell |
| pmid |
sentence |
| 22795133 |
Knockdown of Oct4 or Nanog induced an increase in the expression of Pax6, Gata4, Gata6, Sox17, and FoxA2 in E, H, and p21KD MSCs ( Figure 3F and Figure S2D) |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254627 |
|
|
Homo sapiens |
Embryonic Stem Cell, Embryonic Carcinoma Cell |
| pmid |
sentence |
| 15983365 |
Transfection of NANOG-specific small interfering RNAs reduced levels of NANOG transcript and protein and induced activation of the extraembryonic endoderm-associated genes GATA4, GATA6, LAMININ B1, and AFP as well as upregulation of trophectoderm-associated genes CDX2, GATA2, hCG-alpha, and hCG-beta. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
NANOG | up-regulates quantity by expression
transcriptional regulation
|
NANOG |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-266080 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 16840789 |
We conclude that the Nanog enhancer activity is regulated by both Sall4 and Nanog. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NANOG | down-regulates quantity by repression
transcriptional regulation
|
PAX6 |
0.447 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253164 |
|
|
Homo sapiens |
Mesenchymal Stem Cell |
| pmid |
sentence |
| 22795133 |
Knockdown of Oct4 or Nanog induced an increase in the expression of Pax6, Gata4, Gata6, Sox17, and FoxA2 in E, H, and p21KD MSCs ( Figure 3F and Figure S2D) |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NANOG | down-regulates quantity by repression
transcriptional regulation
|
CDX2 |
0.339 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254622 |
|
|
Homo sapiens |
Embryonic Stem Cell, Embryonic Carcinoma Cell |
| pmid |
sentence |
| 15983365 |
Transfection of NANOG-specific small interfering RNAs reduced levels of NANOG transcript and protein and induced activation of the extraembryonic endoderm-associated genes GATA4, GATA6, LAMININ B1, and AFP as well as upregulation of trophectoderm-associated genes CDX2, GATA2, hCG-alpha, and hCG-beta. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
A6/b1 integrin | up-regulates quantity by expression
|
NANOG |
0.387 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253282 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 18757303 |
Recombinant human LN-511 alone was suffi- cient to enable self-renewal of mouse ES cells for up to 169 days (31 passages). Cells cultured on LN-511 maintained expression of pluripotency markers, such as Oct4, Sox2, Tert, UTF1, and Nanog|ES cells interacted with LN-511 via 1-integrins, mostly a6b1 and aVb1. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
ZNF322 | up-regulates quantity by expression
transcriptional regulation
|
NANOG |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264899 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 24550733 |
Chromatin immunoprecipitation (ChIP) and dual-luciferase reporter assays revealed that Zfp322a binds to Pou5f1 and Nanog promoters and regulates their transcription. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
NANOG | down-regulates quantity by repression
transcriptional regulation
|
GATA4 |
0.454 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254626 |
|
|
Homo sapiens |
Embryonic Stem Cell, Embryonic Carcinoma Cell |
| pmid |
sentence |
| 15983365 |
Transfection of NANOG-specific small interfering RNAs reduced levels of NANOG transcript and protein and induced activation of the extraembryonic endoderm-associated genes GATA4, GATA6, LAMININ B1, and AFP as well as upregulation of trophectoderm-associated genes CDX2, GATA2, hCG-alpha, and hCG-beta. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253162 |
|
|
Homo sapiens |
Mesenchymal Stem Cell |
| pmid |
sentence |
| 22795133 |
Knockdown of Oct4 or Nanog induced an increase in the expression of Pax6, Gata4, Gata6, Sox17, and FoxA2 in E, H, and p21KD MSCs ( Figure 3F and Figure S2D) |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
NANOG | down-regulates quantity by repression
transcriptional regulation
|
SOX17 |
0.484 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253168 |
|
|
Homo sapiens |
Mesenchymal Stem Cell |
| pmid |
sentence |
| 22795133 |
Knockdown of Oct4 or Nanog induced an increase in the expression of Pax6, Gata4, Gata6, Sox17, and FoxA2 in E, H, and p21KD MSCs ( Figure 3F and Figure S2D) |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NANOG | down-regulates quantity by repression
transcriptional regulation
|
AFP |
0.451 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254621 |
|
|
Homo sapiens |
Embryonic Stem Cell, Embryonic Carcinoma Cell |
| pmid |
sentence |
| 15983365 |
Transfection of NANOG-specific small interfering RNAs reduced levels of NANOG transcript and protein and induced activation of the extraembryonic endoderm-associated genes GATA4, GATA6, LAMININ B1, and AFP as well as upregulation of trophectoderm-associated genes CDX2, GATA2, hCG-alpha, and hCG-beta. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SOX17/POU5F1 | up-regulates quantity by expression
transcriptional regulation
|
NANOG |
0.624 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269245 |
|
|
|
|
| pmid |
sentence |
| 31583686 |
Both SOX2 and SOX17 are able to partner with OCT4 and, as a consequence, recognize and bind specific binding motifs.6, 7 In human and mouse ESCs, SOX2/OCT4 bind to canonical motifs (CTTTGTCATGCAAAT-like), which are composite SOX (CATTGTC-like) and OCT (ATGCAAAT-like) motifs|This way SOX17 and SOX2 regulate a common set of pluripotency and GC-related genes (PRDM14, DPPA4, TDGF1, NANOG, LIN28A, TRIM71, OTX2, PIM2) (Fig. 6). Additionally, in TCam-2 cells SOX17 binds to compressed motifs or SOX motifs (not bound by SOX2 in ECs), thereby regulating the PGC specifiers PRDM1 and TFAP2C, the GC-related genes NANOS3 and BMP7 and the cancer-related genes MYC and IGF1 (Fig. 6). In 2102EP cells, SOX2 further binds canonical elements or SOX motifs (not bound by SOX17 in TCam-2), regulating additional pluripotency genes (GDF3, LEFTY2, SALL4, SOX2 and POU5F1) (Fig. 6). |
|
| Publications: |
1 |
| + |
NANOG | down-regulates quantity by repression
transcriptional regulation
|
GATA2 |
0.357 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254625 |
|
|
Homo sapiens |
Embryonic Stem Cell, Embryonic Carcinoma Cell |
| pmid |
sentence |
| 15983365 |
Transfection of NANOG-specific small interfering RNAs reduced levels of NANOG transcript and protein and induced activation of the extraembryonic endoderm-associated genes GATA4, GATA6, LAMININ B1, and AFP as well as upregulation of trophectoderm-associated genes CDX2, GATA2, hCG-alpha, and hCG-beta. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SOX2/POU5F1 | up-regulates quantity by expression
transcriptional regulation
|
NANOG |
0.766 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269237 |
|
|
|
|
| pmid |
sentence |
| 31583686 |
Both SOX2 and SOX17 are able to partner with OCT4 and, as a consequence, recognize and bind specific binding motifs.6, 7 In human and mouse ESCs, SOX2/OCT4 bind to canonical motifs (CTTTGTCATGCAAAT-like), which are composite SOX (CATTGTC-like) and OCT (ATGCAAAT-like) motifs|This way SOX17 and SOX2 regulate a common set of pluripotency and GC-related genes (PRDM14, DPPA4, TDGF1, NANOG, LIN28A, TRIM71, OTX2, PIM2) (Fig. 6). Additionally, in TCam-2 cells SOX17 binds to compressed motifs or SOX motifs (not bound by SOX2 in ECs), thereby regulating the PGC specifiers PRDM1 and TFAP2C, the GC-related genes NANOS3 and BMP7 and the cancer-related genes MYC and IGF1 (Fig. 6). In 2102EP cells, SOX2 further binds canonical elements or SOX motifs (not bound by SOX17 in TCam-2), regulating additional pluripotency genes (GDF3, LEFTY2, SALL4, SOX2 and POU5F1) (Fig. 6). |
|
| Publications: |
1 |
| + |
SALL4 | up-regulates quantity by expression
transcriptional regulation
|
NANOG |
0.787 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-266079 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 16840789 |
We conclude that the Nanog enhancer activity is regulated by both Sall4 and Nanog. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMAD2/SMAD4 | up-regulates quantity by expression
transcriptional regulation
|
NANOG |
0.539 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-242049 |
|
|
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 19279133 |
Here, we show that Smad2/3, the downstream effectors of Activin/Nodal signalling, bind and directly control the activity of the Nanog gene in hESCs. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-242044 |
|
|
Homo sapiens |
Esophageal Squamous Cell Carcinoma Cell |
| pmid |
sentence |
| 18682241 |
We also find that SMADs bind with the NANOG promoter and that SMAD2/3 activity enhances NANOG promoter activity. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
TCF7L1 | down-regulates quantity by repression
transcriptional regulation
|
NANOG |
0.347 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-266081 |
|
|
Mus musculus |
Embryonic Stem Cell Line |
| pmid |
sentence |
| 16894029 |
These experiments showed that Tcf3 is associated with chromatin in the Nanog promoter regions and that the DNA-binding activity of Tcf3 was required for repression. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
POU5F1 | up-regulates quantity by expression
transcriptional regulation
|
NANOG |
0.763 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254939 |
|
|
Homo sapiens |
Embryonic Stem Cell |
| pmid |
sentence |
| 17068183 |
To enhance our understanding of the molecular basis of this differentiation event in humans, we used a functional genomics approach involving RNA interference-mediated suppression of OCT4 function in a human ESC line and analysis of the resulting transcriptional profiles to identify OCT4-dependent genes in human cells. We detected altered expression of >1,000 genes, including targets regulated directly by OCT4 either positively (NANOG, SOX2, REX1, LEFTB, LEFTA/EBAF DPPA4, THY1, and TDGF1) or negatively (CDX2, EOMES, BMP4, TBX18, Brachyury [T], DKK1, HLX1, GATA6, ID2, and DLX5), as well as targets for the OCT4-associated stem cell regulators SOX2 and NANOG. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NANOG | down-regulates quantity by repression
transcriptional regulation
|
LAMB1 |
0.269 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254628 |
|
|
Homo sapiens |
Embryonic Stem Cell, Embryonic Carcinoma Cell |
| pmid |
sentence |
| 15983365 |
Transfection of NANOG-specific small interfering RNAs reduced levels of NANOG transcript and protein and induced activation of the extraembryonic endoderm-associated genes GATA4, GATA6, LAMININ B1, and AFP as well as upregulation of trophectoderm-associated genes CDX2, GATA2, hCG-alpha, and hCG-beta. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
NANOG
- 
- 