Relation Results

Summary

Name KCNJ1
Full Name ATP-sensitive inward rectifier potassium channel 1
Synonyms ATP-regulated potassium channel ROM-K, Inward rectifier K(+) channel Kir1.1, Potassium channel, inwardly rectifying subfamily J member 1 | ROMK1
Primary ID P48048
Links - -
Type protein
Relations 6
Function In the kidney, probably plays a major role in potassium homeostasis. Inward rectifier potassium channels are characterized by a greater tendency to al ...
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Type: Score: Layout: SPV 
0.20.20.3160.309PKCKCNJ1PRKCASRCPRKCD

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▶Modifications Tables

Relations
Regulator
Mechanism
target
score ℹ
+ PKCup-regulates activity img/direct-activation.png phosphorylation KCNJ1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-275990 Ser201 FSKNAVIsKRGGKLC in vitro
pmid sentence
We conclude that ROMK1 is a substrate of PKC and that serine residues 4 and 201 are the two main PKC phosphorylation sites that are essential for the expression of ROMK1 in the cell surface.
Identifier Residue Sequence Organism Cell Line
SIGNOR-275989 Ser4 sSRNVFDT in vitro
pmid sentence
We conclude that ROMK1 is a substrate of PKC and that serine residues 4 and 201 are the two main PKC phosphorylation sites that are essential for the expression of ROMK1 in the cell surface.
Publications: 2 Organism: In Vitro
+ PRKCAdown-regulates activity img/direct_inhibition.png phosphorylation KCNJ1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-276389 Thr193 KKRAKTItFSKNAVI in vitro
pmid sentence
The giant patch clamp together with site direct mutagenesis revealed that Thr-193 is the phosphorylation site on PKC that regulates the pH(i) sensitivity of ROMK1 channels. Mutation of PKC-induced phosphorylation sites (T193A) decreases the pH(i) sensitivity and increases the interaction of channel-PIP(2). 
Publications: 1 Organism: In Vitro
+ SRCdown-regulates img/direct_inhibition.png phosphorylation KCNJ1 0.316
Identifier Residue Sequence Organism Cell Line
SIGNOR-92513 Tyr337 SKTKEGKyRVDFHNF Homo sapiens
pmid sentence
Addition of active c-src and [32p]atp to the purified romk1 protein resulted in the phosphorylation of the romk1 protein. However, c-src did not phosphorylate r1y337a in which tyrosine residue 337 was mutated to alanine. Furthermore, phosphopeptide mapping identified two phosphopeptides from the trypsin-digested romk1 protein.
Identifier Residue Sequence Organism Cell Line
SIGNOR-97803 Tyr337 SKTKEGKyRVDFHNF Homo sapiens
pmid sentence
Inhibition of c-src with herbimycin a significantly decreased the tyrosine phosphorylation level of romk1... tyrosine dephosphorylation enhances the exocytosis of romk1
Publications: 2 Organism: Homo Sapiens
+ PRKCDup-regulates activity img/direct-activation.png KCNJ1 0.309
Identifier Residue Sequence Organism Cell Line
SIGNOR-248943 Homo sapiens
pmid sentence
To determine whether this channel is a substrate for PKA, ROMK tagged with the hemagglutinin epitope was transiently transfected into HEK293 cells. In vitro labeling of immunoprecipitated proteins from transfected cells showed that ROMK could be phosphorylated by PKA. | Taken together, these results provide strong evidence that direct phosphorylation of the channel polypeptide by PKA is involved in channel regulation and PKA-dependent phosphorylation is essential for ROMK channel activity.
Publications: 1 Organism: Homo Sapiens
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