| + |
MAPK1 |
phosphorylation
|
DOCK1 |
0.257 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262769 |
Thr1772 |
QQTPPPVtPRAKLSF |
Mus musculus |
NIH-3T3 Cell |
| pmid |
sentence |
| 22028470 |
We have optimized a chemical genetic system using analog-sensitive ERK2, a form of ERK2 engineered to use an analog of adenosine 5'-triphosphate (ATP), to tag and isolate ERK2 substrates in vitro. This approach identified 80 proteins phosphorylated by ERK2, 13 of which are known ERK2 substrates. With this improved methodology, we detected 98 sites directly phosphorylated by ERK2 on 80 proteins from NIH 3T3-L1 fibroblasts. Thirteen of these proteins are known substrates and the rest represent previously unknown kinase/substrate interactions. (table1) |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
DOCK1 | up-regulates activity 
guanine nucleotide exchange factor
|
RAC1 |
0.722 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-266822 |
|
|
Mus musculus |
N1E-115 Cell |
| pmid |
sentence |
| 25533347 |
We found in this study that AUTS2 is involved in Rac1 activation via P-Rex1 and the Elmo2/Dock180 complex, but not STEF or Tiam1, for the lamellipodia formation in N1E-115 cells. However, the enhancement of neurite elongation in primary neurons by AUTS2 expression is specifically mediated by the Elmo2/Dock180 complex. These results suggested that several Rac-GEFs differentially or cooperatively participate in Rac1 activation to promote neuronal migration and neurite outgrowth. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
SNX5 | up-regulates activity
binding
|
DOCK1 |
0.358 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-269441 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 18596235 |
SNX5 Is a Novel Binding Partner of the DHR1 Domain of DOCK180. In summary, we found that DOCK180 regulates transport of CI-MPR via SNX5 binding. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
AUTS2 | up-regulates activity
binding
|
DOCK1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-266820 |
|
|
Mus musculus |
N1E-115 Cell |
| pmid |
sentence |
| 25533347 |
Mutations in the Autism susceptibility candidate 2 gene (AUTS2), whose protein is believed to act in neuronal cell nuclei, have been associated with multiple psychiatric illnesses, including autism spectrum disorders, intellectual disability, and schizophrenia. Here we show that cytoplasmic AUTS2 is involved in the regulation of the cytoskeleton and neural development. AUTS2 activates Rac1 to induce lamellipodia but downregulates Cdc42 to suppress filopodia. Our loss-of-function and rescue experiments show that a cytoplasmic AUTS2-Rac1 pathway is involved in cortical neuronal migration and neuritogenesis in the developing brain. These results suggest that FL-AUTS2 can activate Rac1 via interaction with P-Rex1 and the Elmo2/Dock180 complex to regulate actin dynamics in N1E-115 cells. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
3.0
DOCK1
- 
- 