| + |
MAPK8 | down-regulates quantity by destabilization 
phosphorylation
|
NFE2 |
0.42 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-275552 |
Ser157 |
LNYSDAEsLELEGTE |
|
|
| pmid |
sentence |
| 19966288 |
Through use of different approaches including nano-scale proteomics, we show that activated-JNK, or Phospho-JNK (P-JNK), physically interacts with p45/NF-E2 and phosphorylates its Ser157 residue. This reaction leads to the poly-ubiquitination of p45/NF-E2 at one or more of six Lys residues, one of which being also a sumoylation site, and its degradation through the proteasome pathway. |
|
| Publications: |
1 |
| + |
NFE2 | up-regulates quantity by expression 
transcriptional regulation
|
HBD |
0.365 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-251843 |
|
|
Mus musculus |
Erythroleukemia Cell |
| pmid |
sentence |
| 16287851 |
NF-E2 is a transcription activator for the regulation of a number of erythroid- and megakaryocytic lineage-specific genes. Here we present evidence that the large subunit of mammalian NF-E2, p45, is sumoylated in vivo in human erythroid K562 cells. we demonstrated by stable transfection assay that only the wild-type p45, but not its mutant form p45 (K368R), could efficiently rescue β-globin gene expression in the p45-null, erythroid cell line CB3. These data together point to a model of mammalian β-like globin gene activation by sumoylated p45/NF-E2 in erythroid cells. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
NFE2 | up-regulates quantity by expression
transcriptional regulation
|
HBG2 |
0.51 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-251840 |
|
|
Mus musculus |
Erythroleukemia Cell |
| pmid |
sentence |
| 16287851 |
NF-E2 is a transcription activator for the regulation of a number of erythroid- and megakaryocytic lineage-specific genes. Here we present evidence that the large subunit of mammalian NF-E2, p45, is sumoylated in vivo in human erythroid K562 cells. we demonstrated by stable transfection assay that only the wild-type p45, but not its mutant form p45 (K368R), could efficiently rescue β-globin gene expression in the p45-null, erythroid cell line CB3. These data together point to a model of mammalian β-like globin gene activation by sumoylated p45/NF-E2 in erythroid cells. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
NFE2 | up-regulates quantity by expression
transcriptional regulation
|
HBE1 |
0.479 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-251842 |
|
|
Mus musculus |
Erythroleukemia Cell |
| pmid |
sentence |
| 16287851 |
NF-E2 is a transcription activator for the regulation of a number of erythroid- and megakaryocytic lineage-specific genes. Here we present evidence that the large subunit of mammalian NF-E2, p45, is sumoylated in vivo in human erythroid K562 cells. we demonstrated by stable transfection assay that only the wild-type p45, but not its mutant form p45 (K368R), could efficiently rescue β-globin gene expression in the p45-null, erythroid cell line CB3. These data together point to a model of mammalian β-like globin gene activation by sumoylated p45/NF-E2 in erythroid cells. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
NFE2 | up-regulates quantity by expression
transcriptional regulation
|
HBG1 |
0.508 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-251841 |
|
|
Mus musculus |
Erythroleukemia Cell |
| pmid |
sentence |
| 16287851 |
NF-E2 is a transcription activator for the regulation of a number of erythroid- and megakaryocytic lineage-specific genes. Here we present evidence that the large subunit of mammalian NF-E2, p45, is sumoylated in vivo in human erythroid K562 cells. we demonstrated by stable transfection assay that only the wild-type p45, but not its mutant form p45 (K368R), could efficiently rescue β-globin gene expression in the p45-null, erythroid cell line CB3. These data together point to a model of mammalian β-like globin gene activation by sumoylated p45/NF-E2 in erythroid cells. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
NFE2 | up-regulates quantity by expression
transcriptional regulation
|
HBB |
0.493 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-251839 |
|
|
Mus musculus |
Erythroleukemia Cell |
| pmid |
sentence |
| 16287851 |
NF-E2 is a transcription activator for the regulation of a number of erythroid- and megakaryocytic lineage-specific genes. Here we present evidence that the large subunit of mammalian NF-E2, p45, is sumoylated in vivo in human erythroid K562 cells. we demonstrated by stable transfection assay that only the wild-type p45, but not its mutant form p45 (K368R), could efficiently rescue β-globin gene expression in the p45-null, erythroid cell line CB3. These data together point to a model of mammalian β-like globin gene activation by sumoylated p45/NF-E2 in erythroid cells. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
ITCH | down-regulates activity
ubiquitination
|
NFE2 |
0.423 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-275553 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 18718448 |
Itch regulates p45/NF-E2 in vivo by Lys63-linked ubiquitination| Interestingly, Itch suppressed the transactivation activity of p45/NF-E2 by adding a Lys63-linked polyubiquitin chain. Confocal microscopy revealed that ubiquitinated p45/NF-E2 became localized in the cytoplasm when Itch was over-expressed. Thus, Itch-mediated ubiquitination of p45/NF-E2 does not target the protein for proteasomal degradation, but instead retains p45/NF-E2 in the cytoplasm, where it cannot function as a transactivator. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
NFE2
- 
- 