| + |
TRIM65 | up-regulates activity 
ubiquitination
|
IFIH1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278535 |
Lys743 |
QWITENEkFAEVGVK |
Homo sapiens |
|
| pmid |
sentence |
| 28031478 |
These results indicate that TRIM65 promotes MDA5 ubiquitination at lysine 743, which is important for MDA5 activation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
Ub:E2 | up-regulates activity
ubiquitination
|
TRIM65 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271171 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 34199813 |
The ubiquitination process is mediated sequentially by three classes of enzymes consisting of a Ub-activating enzyme E1, a Ub-conjugating enzyme E2, and a Ub ligase E3. Ub is first activated by E1 in an adenosine 5′-triphosphate (ATP)-dependent manner t |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
UBE2D1 | up-regulates activity
ubiquitination
|
TRIM65 |
0.303 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272175 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 24778252 |
Ubiquitination assays demonstrate that TRIM65 is an ubiquitin E3 ligase for TNRC6 proteins. The combination of overexpression and knockdown studies establishes that TRIM65 relieves miRNA-driven suppression of mRNA expression through ubiquitination and subsequent degradation of TNRC6. TRIM65 regulates ubiquitination and stability of TNRC6. (A) In vitro ubiquitination of TNRC6A by TRIM65 plus E1, E2 (UBCH5A, also known as UBE2D1), ATP, and HA-Ub. GST-tagged TRIM65 and mutant TRIM65 were purified from bacteria. TRIM65 regulates ubiquitination and stability of TNRC6. (A) In vitro ubiquitination of TNRC6A by TRIM65 plus E1, E2 (UBCH5A, also known as UBE2D1), ATP, and HA-Ub. GST-tagged TRIM65 and mutant TRIM65 were purified from bacteria. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TRIM65 | down-regulates quantity by destabilization 
polyubiquitination
|
ARHGAP35 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272256 |
|
|
Homo sapiens |
KM-12 Cell |
| pmid |
sentence |
| 31332286 |
Ubiquitin ligase TRIM65 promotes colorectal cancer metastasis by targeting ARHGAP35 for protein degradation |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TRIM65 | down-regulates activity
ubiquitination
|
NLRP3 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278566 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 34512673 |
These results suggest that TRIM65 could inhibit the activation of the NLRP3 inflammasome in response to multiple agonists.|Thus, TRIM65 deficiency impairs NLRP3 ubiquitination and enhances NLRP3 inflammasome activation, but has no effects on AIM2 or IPAF inflammasome activation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TRIM65 | down-regulates quantity by destabilization
polyubiquitination
|
TNRC6A |
0.455 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272174 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 24778252 |
Ubiquitination assays demonstrate that TRIM65 is an ubiquitin E3 ligase for TNRC6 proteins. The combination of overexpression and knockdown studies establishes that TRIM65 relieves miRNA-driven suppression of mRNA expression through ubiquitination and subsequent degradation of TNRC6. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
TRIM65
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