| + |
Cullin4-RBX1-DDB1 | up-regulates activity 
polyubiquitination
|
OPTN |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272209 |
Lys501 |
LQLAVLLkENDAFED |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 32014991 |
DCAF4-mediated ubiquitination of OPTN facilitates the degradation of DBR-exposed SOD1. OPTN is involved in degradation of DBR-exposed SOD1. These data demonstrate that DCAF4-including CRL4 complex mediates OPTN ubiquitination at lysine 501, and this ubiquitination is absent in the ALS-related OPTNmut. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TBK1 | up-regulates activity
phosphorylation
|
OPTN |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278169 |
Ser177 |
SSGSSEDsFVEIRMA |
Homo sapiens |
|
| pmid |
sentence |
| 26365381 |
Given that TBK1 can phosphorylate OPTN on S177, S473 and S513 in response to mitochondrial depolarization, we explored the function of these events in vivo.|Phosphorylation of OPTN on S473 and S513 promotes TBK1 activation and recruitment of OPTN to depolarized mitochondria. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278170 |
Ser473 |
AQMEVYCsDFHAERA |
Homo sapiens |
|
| pmid |
sentence |
| 26365381 |
Given that TBK1 can phosphorylate OPTN on S177, S473 and S513 in response to mitochondrial depolarization, we explored the function of these events in vivo.|Phosphorylation of OPTN on S473 and S513 promotes TBK1 activation and recruitment of OPTN to depolarized mitochondria. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278171 |
Ser513 |
FEDGGRQsLMEMQSR |
Homo sapiens |
|
| pmid |
sentence |
| 26365381 |
Given that TBK1 can phosphorylate OPTN on S177, S473 and S513 in response to mitochondrial depolarization, we explored the function of these events in vivo.|Phosphorylation of OPTN on S473 and S513 promotes TBK1 activation and recruitment of OPTN to depolarized mitochondria. |
|
| Publications: |
3 |
Organism: |
Homo Sapiens |
| + |
PLK1 | up-regulates
phosphorylation
|
OPTN |
0.525 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-196367 |
Ser177 |
SSGSSEDsFVEIRMA |
Homo sapiens |
|
| pmid |
sentence |
| 22365832 |
Here we show that at mitotic entry, plk1 phosphorylates optineurin (optn) at serine 177 and that this dissociates optn from the golgi-localized gtpase rab8, inducing its translocation into the nucleus. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DCAF4 | up-regulates activity
binding
|
OPTN |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272210 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 32014991 |
DCAF4-mediated ubiquitination of OPTN facilitates the degradation of DBR-exposed SOD1. OPTN is involved in degradation of DBR-exposed SOD1. These data demonstrate that DCAF4-including CRL4 complex mediates OPTN ubiquitination at lysine 501, and this ubiquitination is absent in the ALS-related OPTNmut. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
OPTN | up-regulates quantity by expression 
transcriptional regulation
|
BDNF |
0.283 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-259878 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 22194658 |
SiRNA effectively downregulated optineurin expression in RGC-5 and PC12 stable transfected cells. Optineurin siRNA significantly inhibited cell growth and increased apoptosis in RGC-5 and PC12 cells. Microarray analysis identified 112 differentially expressed genes in optineurin siRNA transfected RGC-5 cells. Quantitative real-time PCR and western blot confirmed that the expression of brain-derived neurotrophic factor (Bdnf), neurotrophin-3(Ntf3), synaptosomal-associated protein 25(Snap25), and neurofilament, light polypeptide(Nefl) was significantly downregulated in RGC-5 and PC12 cells transfected with optineurin siRNA. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
OPTN | up-regulates quantity by expression
transcriptional regulation
|
SNAP25 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-259880 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 22194658 |
SiRNA effectively downregulated optineurin expression in RGC-5 and PC12 stable transfected cells. Optineurin siRNA significantly inhibited cell growth and increased apoptosis in RGC-5 and PC12 cells. Microarray analysis identified 112 differentially expressed genes in optineurin siRNA transfected RGC-5 cells. Quantitative real-time PCR and western blot confirmed that the expression of brain-derived neurotrophic factor (Bdnf), neurotrophin-3(Ntf3), synaptosomal-associated protein 25(Snap25), and neurofilament, light polypeptide(Nefl) was significantly downregulated in RGC-5 and PC12 cells transfected with optineurin siRNA. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
OPTN | up-regulates
|
Protein_aggregates |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262275 |
|
|
|
|
| pmid |
sentence |
| 27181519 |
Optineurin and TBK1 have also been discovered co-localizing in protein aggregates, and the phosphorylation of optineurin at serine 177 has been shown to be critical to its function in mediating clearance of aggregated proteins via autophagy |
|
| Publications: |
1 |
| + |
OPTN | up-regulates quantity by expression
transcriptional regulation
|
NEFL |
0.271 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-259881 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 22194658 |
SiRNA effectively downregulated optineurin expression in RGC-5 and PC12 stable transfected cells. Optineurin siRNA significantly inhibited cell growth and increased apoptosis in RGC-5 and PC12 cells. Microarray analysis identified 112 differentially expressed genes in optineurin siRNA transfected RGC-5 cells. Quantitative real-time PCR and western blot confirmed that the expression of brain-derived neurotrophic factor (Bdnf), neurotrophin-3(Ntf3), synaptosomal-associated protein 25(Snap25), and neurofilament, light polypeptide(Nefl) was significantly downregulated in RGC-5 and PC12 cells transfected with optineurin siRNA. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
OPTN | up-regulates quantity by expression
transcriptional regulation
|
NTF3 |
0.25 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-259879 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 22194658 |
SiRNA effectively downregulated optineurin expression in RGC-5 and PC12 stable transfected cells. Optineurin siRNA significantly inhibited cell growth and increased apoptosis in RGC-5 and PC12 cells. Microarray analysis identified 112 differentially expressed genes in optineurin siRNA transfected RGC-5 cells. Quantitative real-time PCR and western blot confirmed that the expression of brain-derived neurotrophic factor (Bdnf), neurotrophin-3(Ntf3), synaptosomal-associated protein 25(Snap25), and neurofilament, light polypeptide(Nefl) was significantly downregulated in RGC-5 and PC12 cells transfected with optineurin siRNA. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
OPTN | down-regulates 
|
Apoptosis |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-259876 |
|
|
Mus musculus |
RGC-5 Cell |
| pmid |
sentence |
| 22194658 |
SiRNA effectively downregulated optineurin expression in RGC-5 and PC12 stable transfected cells. Optineurin siRNA significantly inhibited cell growth and increased apoptosis in RGC-5 and PC12 cells. Microarray analysis identified 112 differentially expressed genes in optineurin siRNA transfected RGC-5 cells. Quantitative real-time PCR and western blot confirmed that the expression of brain-derived neurotrophic factor (Bdnf), neurotrophin-3(Ntf3), synaptosomal-associated protein 25(Snap25), and neurofilament, light polypeptide(Nefl) was significantly downregulated in RGC-5 and PC12 cells transfected with optineurin siRNA. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
HACE1 | down-regulates quantity by destabilization 
ubiquitination
|
OPTN |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278748 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 25026213 |
Here we report that tumor suppressor HACE1, a ubiquitin ligase, ubiquitylates OPTN and promotes its interaction with p62 and SQSTM1 to form the autophagy receptor complex, thus accelerating autophagic flux.|Ubiquitylation of Autophagy Receptor Optineurin by HACE1 Activates Selective Autophagy for Tumor Suppression.|HACE1 mediated K48 linked poly-Ub chains targets OPTN for autophagic degradation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
OPTN | up-regulates
|
Cell_growth |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-259877 |
|
|
Mus musculus |
RGC-5 Cell |
| pmid |
sentence |
| 22194658 |
SiRNA effectively downregulated optineurin expression in RGC-5 and PC12 stable transfected cells. Optineurin siRNA significantly inhibited cell growth and increased apoptosis in RGC-5 and PC12 cells. Microarray analysis identified 112 differentially expressed genes in optineurin siRNA transfected RGC-5 cells. Quantitative real-time PCR and western blot confirmed that the expression of brain-derived neurotrophic factor (Bdnf), neurotrophin-3(Ntf3), synaptosomal-associated protein 25(Snap25), and neurofilament, light polypeptide(Nefl) was significantly downregulated in RGC-5 and PC12 cells transfected with optineurin siRNA. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
OPTN | up-regulates activity
binding
|
TBK1 |
0.787 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-262276 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 20174559 |
Using biochemical experiments we showed that optineurin interacts with the protein kinase TBK1 and the ubiquitin ligase TRAF3. Furthermore, a mutation in optineurin that prevents the interaction with the small protein modifier ubiquitin (D474N) ablated the negative regulatory function of optineurin. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
OPTN
- 
- 