| + |
CDK2 | up-regulates activity 
phosphorylation
|
NFYA |
0.45 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250742 |
Ser320 |
GEGGRFFsPKEKDSP |
|
|
| pmid |
sentence |
| 12857729 |
Cdk2 phosphorylates two serine residues near the DNA-binding domain of the YA subunit of NF-Y. Cyclin A-cdk2 appears to associate with NF-Y both in vitro and in vivo. Furthermore, YA protein is phosphorylated in parallel with a cell cycle-dependent activation of cdk2 kinase and cyclin A expression. YA phosphorylation is unnecessary for heterotrimer formation with the YB-YC dimer. However, NF-Y containing a phosphorylation-deficient mutant form of YA, YA-aa, has its DNA binding activity impaired. \ To examine whether cdk2 phosphorylates the two serine residues at positions 320 and 326 in YA, we replaced either or both with alanine by site-directed mutagenesis. In a kinase assay using purified GST fusion proteins in vitro, cdk2 phosphorylated the wild type and both of the single-mutant proteins (YA-as and -sa), but not the double-mutant protein (YA-aa) |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250743 |
Ser326 |
FSPKEKDsPHMQDPN |
|
|
| pmid |
sentence |
| 12857729 |
Cdk2 phosphorylates two serine residues near the DNA-binding domain of the YA subunit of NF-Y. Cyclin A-cdk2 appears to associate with NF-Y both in vitro and in vivo. Furthermore, YA protein is phosphorylated in parallel with a cell cycle-dependent activation of cdk2 kinase and cyclin A expression. YA phosphorylation is unnecessary for heterotrimer formation with the YB-YC dimer. However, NF-Y containing a phosphorylation-deficient mutant form of YA, YA-aa, has its DNA binding activity impaired. \ To examine whether cdk2 phosphorylates the two serine residues at positions 320 and 326 in YA, we replaced either or both with alanine by site-directed mutagenesis. In a kinase assay using purified GST fusion proteins in vitro, cdk2 phosphorylated the wild type and both of the single-mutant proteins (YA-as and -sa), but not the double-mutant protein (YA-aa) |
|
| Publications: |
2 |
| + |
NFYA | up-regulates activity
binding
|
SP1 |
0.6 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254816 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 12427542 |
Our results further confirm the important transactivating role for NF-Y for the CBS-1b promoter, via its synergism with Sp1. While differential phosphorylation of Sp1 likely contributes to binding to multiple GC-/GT-boxes in the CBS-1b and promoter activation [16], NF-Y is clearly necessary for a maximal activation response. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NFYA | up-regulates quantity by expression 
transcriptional regulation
|
CBS |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254815 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 12427542 |
Our results further confirm the important transactivating role for NF-Y for the CBS-1b promoter, via its synergism with Sp1. While differential phosphorylation of Sp1 likely contributes to binding to multiple GC-/GT-boxes in the CBS-1b and promoter activation [16], NF-Y is clearly necessary for a maximal activation response. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NFYA | up-regulates quantity by expression
transcriptional regulation
|
GFI1B |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254434 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19965638 |
HMGB2 binds to the GFI1B promoter in vivo and up-regulates its trans-activation most likely by enhancing the binding of Oct-1 and, to a lesser extent, of GATA-1 and NF-Y to the GFI1B promoter. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NFYA | up-regulates quantity by expression
transcriptional regulation
|
SOX18 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254818 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 18496767 |
co-transfection experiments revealed that over-expression of Sp3 and ZBP-89 down-regulate, while over-expression of NF-Y up-regulates SOX18 promoter activity in HeLa cells |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NFYA | form complex 
binding
|
NFY |
0.963 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-63013 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 9885213 |
Nf-y is one of the best characterized ccaat binding proteins, and its unique structure and evolutionary conservation suggest that it plays a crucial role in transcription of eukaryotic genes.It Is a ubiquitous heteromeric transcription factor, composed of three subunits, nf-ya, nf-yb, and nf-yc, all necessary for dna binding. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NFYA | up-regulates quantity by expression
transcriptional regulation
|
GCH1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-252232 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 15496512 |
Coactivator RNF4 is involved in the GCH gene expression. Through serial deletion and mutagenesis studies of the GCH promoter, we defined the RNF4-responsive element on GCH proximal promoter as a CCAAT box. RNF4 did not possess specific DNA binding activity toward this CCAAT box, which suggests that RNF4 may be a coactivator of the CCAAT boxbinding protein nuclear factor Y (NF-Y). RNF4 is a coactivator for nuclear factor Y on GTP cyclohydrolase I proximal promoter. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NFYA | up-regulates quantity by expression
transcriptional regulation
|
OGG1 |
0.268 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254817 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 14688259 |
these results demonstrate that MMS can up-regulate hOGG1 expression through the induction of the transcription factor, NF-YA, and increased transcription level of the hOGG1 gene correlates with an increase in enzyme activity providing functional protection from MMS. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NFYA | up-regulates quantity by expression
transcriptional regulation
|
PHGDH |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-255209 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 18378410 |
Positive regulation of promoter activity of human 3-phosphoglycerate dehydrogenase (PHGDH) gene is mediated by transcription factors Sp1 and NF-Y. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RNF4 | up-regulates activity
binding
|
NFYA |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-252229 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 15496512 |
Coactivator RNF4 is involved in the GCH gene expression. Through serial deletion and mutagenesis studies of the GCH promoter, we defined the RNF4-responsive element on GCH proximal promoter as a CCAAT box. RNF4 did not possess specific DNA binding activity toward this CCAAT box, which suggests that RNF4 may be a coactivator of the CCAAT boxbinding protein nuclear factor Y (NF-Y). RNF4 is a coactivator for nuclear factor Y on GTP cyclohydrolase I proximal promoter. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
NFYA
- 
- 