| + |
USP28 | up-regulates quantity by stabilization 
deubiquitination
|
UCK1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-275855 |
Lys81 |
EQKAKALkGQYNFDH |
|
|
| pmid |
sentence |
| 31938050 |
We demonstrated that the ubiquitin E3 ligase KLHL2 interacted with UCK1 and mediated its polyubiquitination at the K81 residue and degradation. We showed that deubiquitinase USP28 antagonized KLHL2-mediated polyubiquitylation of UCK1. |
|
| Publications: |
1 |
| + |
ATM | down-regulates activity 
phosphorylation
|
USP28 |
0.312 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-275854 |
Ser495 |
STSTESSsQDVESTF |
|
|
| pmid |
sentence |
| 31938050 |
Once all the three conservative SQ sites (S67, S495, and S714), in USP28, were mutated to alanine, the pS/TQ antibody completely could not recognize the immunoprecipitated Flag-USP28-3S/A (Figure Figure55D), suggesting that in response to 5'-AZA-induced stress, ATM phosphorylates USP28 at all these three sites.|We demonstrated that the ubiquitin E3 ligase KLHL2 interacted with UCK1 and mediated its polyubiquitination at the K81 residue and degradation. We showed that deubiquitinase USP28 antagonized KLHL2-mediated polyubiquitylation of UCK1. We also provided evidence that ATM-mediated phosphorylation of USP28 resulted in its disassociation from KLHL2 and UCK1 destabilization. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-275852 |
Ser67 |
DERVKEPsQDTVATE |
|
|
| pmid |
sentence |
| 31938050 |
Once all the three conservative SQ sites (S67, S495, and S714), in USP28, were mutated to alanine, the pS/TQ antibody completely could not recognize the immunoprecipitated Flag-USP28-3S/A (Figure Figure55D), suggesting that in response to 5'-AZA-induced stress, ATM phosphorylates USP28 at all these three sites.|We demonstrated that the ubiquitin E3 ligase KLHL2 interacted with UCK1 and mediated its polyubiquitination at the K81 residue and degradation. We showed that deubiquitinase USP28 antagonized KLHL2-mediated polyubiquitylation of UCK1. We also provided evidence that ATM-mediated phosphorylation of USP28 resulted in its disassociation from KLHL2 and UCK1 destabilization. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-275853 |
Ser714 |
ESSTNSSsQDYSTSQ |
|
|
| pmid |
sentence |
| 31938050 |
Once all the three conservative SQ sites (S67, S495, and S714), in USP28, were mutated to alanine, the pS/TQ antibody completely could not recognize the immunoprecipitated Flag-USP28-3S/A (Figure Figure55D), suggesting that in response to 5'-AZA-induced stress, ATM phosphorylates USP28 at all these three sites.|We demonstrated that the ubiquitin E3 ligase KLHL2 interacted with UCK1 and mediated its polyubiquitination at the K81 residue and degradation. We showed that deubiquitinase USP28 antagonized KLHL2-mediated polyubiquitylation of UCK1. We also provided evidence that ATM-mediated phosphorylation of USP28 resulted in its disassociation from KLHL2 and UCK1 destabilization. |
|
| Publications: |
3 |
| + |
ATR | up-regulates activity
phosphorylation
|
USP28 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-275850 |
Ser67 |
DERVKEPsQDTVATE |
|
|
| pmid |
sentence |
| 31938050 |
Here we report that the deubiquitylase USP28 is recruited to sites of DNA damage in cisplatin-treated cells. ATR phosphorylates USP28 and increases its enzymatic activity.|Representative immunoblots of n = 3. C Immunoblotting of total and phosphorylated USP28 at serine 67 and 714 in A431 cells exposed to indicated concentrations of CPPD for 6 h. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-275851 |
Ser714 |
ESSTNSSsQDYSTSQ |
|
|
| pmid |
sentence |
| 31938050 |
Here we report that the deubiquitylase USP28 is recruited to sites of DNA damage in cisplatin-treated cells. ATR phosphorylates USP28 and increases its enzymatic activity.|Representative immunoblots of n = 3. C Immunoblotting of total and phosphorylated USP28 at serine 67 and 714 in A431 cells exposed to indicated concentrations of CPPD for 6 h. |
|
| Publications: |
2 |
| + |
ATM | up-regulates activity
phosphorylation
|
USP28 |
0.312 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279007 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 31938050 |
These novel findings establish a direct connection between the ubiquitination of UCK1 by KLHL2 and phosphorylation of USP28 and UCK1 by ATM, which are involved in mediating drug resistance against 5'-AZA in AML patients. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
USP28 | up-regulates
deubiquitination
|
MYC |
0.704 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-155590 |
|
|
Homo sapiens |
Breast Cancer Cell |
| pmid |
sentence |
| 17558397 |
Usp28, an ubiquitin-specific protease, binds to myc through an interaction with fbw7alpha, an f-box protein that is part of an scf-type ubiquitin ligase. Therefore, it stabilizes myc. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
USP28 | up-regulates quantity by stabilization
deubiquitination
|
CDH1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-274057 |
|
|
|
|
| pmid |
sentence |
| 18662541 |
Usp28 deubiquitylates and consequently stabilizes Claspin in response to DNA damage |
|
| Publications: |
1 |
4.0
USP28
- 
- 