| + |
MARCHF5 | down-regulates quantity by destabilization 
ubiquitination
|
PRKN |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278554 |
Lys220 |
GAHPTSDkETSVALH |
Homo sapiens |
|
| pmid |
sentence |
| 33565245 |
MITOL promotes cell survival by degrading Parkin during mitophagy.|Mechanistically, MITOL mediates ubiquitination of Parkin at lysine 220 residue, which promotes its proteasomal degradation, and thereby fine-tunes mitophagy by controlling the quantity of Parkin. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MARCHF5 | down-regulates activity
ubiquitination
|
ERN1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278609 |
Lys481 |
LQHQQFQkELEKIQL |
Homo sapiens |
|
| pmid |
sentence |
| 31368599 |
MITOL promotes K63-linked chain ubiquitination of IRE1\u03b1 at lysine 481 (K481), thereby preventing hyper-oligomerization of IRE1\u03b1 and regulated IRE1\u03b1-dependent decay (RIDD). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MARCHF5 | down-regulates quantity by destabilization
polyubiquitination
|
MARCHF5 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271895 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 16874301 |
Rapid degradation of MITOL by autoubiquitination activity. Taken together, these results suggested that MITOL strictly controls its protein expression level by rapid degradation of MITOL through the PHD-dependent autoubiquitination activity. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MARCHF5 | down-regulates quantity by destabilization
polyubiquitination
|
DNM1L |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271894 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 16874301 |
We found that MITOL associated with and ubiquitinated mitochondrial fission protein hFis1 and Drp1.Pulse–chase experiment also indicated that MITOL overexpression promoted Drp1 turnover. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MARCHF5 | down-regulates quantity by destabilization
ubiquitination, polyubiquitination
|
FIS1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-274141 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 16874301 |
MITOL associates with and ubiquitinates mitochondrial fission protein hFis1. (A) Ubiquitination of hFis1 by MITOL. Thus, MITOL may control the protein expression level of hFis1 through the ubiquitin–proteasome pathway. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271896 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 16874301 |
MITOL associates with and ubiquitinates mitochondrial fission protein hFis1. (A) Ubiquitination of hFis1 by MITOL. Thus, MITOL may control the protein expression level of hFis1 through the ubiquitin–proteasome pathway. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
Ub:E2 | up-regulates activity 
ubiquitination
|
MARCHF5 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271247 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 34199813 |
The ubiquitination process is mediated sequentially by three classes of enzymes consisting of a Ub-activating enzyme E1, a Ub-conjugating enzyme E2, and a Ub ligase E3. Ub is first activated by E1 in an adenosine 5′-triphosphate (ATP)-dependent manner t |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MARCHF5 | down-regulates quantity by destabilization
ubiquitination
|
MFN1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-274133 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 20103533 |
MARCH5, a mitochondrial E3 ubiquitin ligase, has been identified as a molecule that binds mitochondrial fission 1 protein (hFis1), dynamin-related protein 1 (Drp1) and mitofusin 2 (Mfn2), key proteins in the control of mitochondrial fission and fusion.|Notably, a significant increase in Mfn1 level, but not Mfn2, Drp1 or hFis1 levels, was observed in MARCH5-depleted cells, indicating that Mfn1 is a major ubiquitylation substrate. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278569 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23253261 |
Mfn1 is ubiquitylated by MARCH5 at G 2 / M phase and degraded in a proteasome dependent manner. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272981 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 20103533 |
MARCH5, a mitochondrial E3 ubiquitin ligase, has been identified as a molecule that binds mitochondrial fission 1 protein (hFis1), dynamin-related protein 1 (Drp1) and mitofusin 2 (Mfn2), key proteins in the control of mitochondrial fission and fusion.|Notably, a significant increase in Mfn1 level, but not Mfn2, Drp1 or hFis1 levels, was observed in MARCH5-depleted cells, indicating that Mfn1 is a major ubiquitylation substrate. |
|
| Publications: |
3 |
Organism: |
Homo Sapiens |
| + |
MARCHF5 | up-regulates activity
ubiquitination
|
MFN2 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278553 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23727017 |
Taken together, these results suggested that MITOL regulates endoplasmic reticulum tethering to mitochondria by activating Mfn2 via K192 ubiquitination.|Therefore, MITOL specifically ubiquitinates mitochondrial Mfn2. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MARCHF5 | down-regulates quantity
ubiquitination
|
SOD1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278654 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19741096 |
Cycloheximide-chase assay in the Neuro2a cells indicated that MITOL overexpression promoted mSOD1 degradation and suppressed both the mitochondrial accumulation of mSOD1 and mSOD1-induced reactive oxygen species generation.|In vitro ubiquitination assay revealed that MITOL directly ubiquitinates mSOD1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MARCHF5 | down-regulates quantity by destabilization
ubiquitination
|
MCL1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278700 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 32015503 |
MARCH5 promotes ubiquitination of MCL1 and NOXA.|Together, this suggests that degradation of MCL1 by MARCH5 depends on binding to NOXA, but degradation of NOXA may be promoted by additional E3-ligases if MCL1 levels become limited, or can simply no longer be degraded. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MARCHF5 | down-regulates quantity
ubiquitination
|
PMAIP1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278758 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 32015503 |
MARCH5 promotes ubiquitination of MCL1 and NOXA.|Of note, MARCH5 depletion led to the accumulation of MCL1 and NOXA in asynchronous as well as G2 cells, suggesting that MARCH5 controls NOXA/MCL1 levels throughout the cell cycle. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MARCHF5 | down-regulates quantity by destabilization
ubiquitination
|
SOD1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272982 |
|
|
Chlorocebus aethiops |
COS Cell |
| pmid |
sentence |
| 19741096 |
Mitochondrial ubiquitin ligase MITOL ubiquitinates mutant SOD1 and attenuates mutant SOD1-induced reactive oxygen species generation |
|
| Publications: |
1 |
Organism: |
Chlorocebus Aethiops |
3.0
MARCHF5
- 
- 