| + |
MAPK1 | up-regulates activity 
phosphorylation
|
ALOX5 |
0.397 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264439 |
Ser272 |
CSLERQLsLEQEVQQ |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 12670876 |
Intriguingly, a significant difference in the potency of nonredox-type inhibitors (but not of BWA4C) was determined between wild-type 5-LO and the mutant S271A/S663A-5-LO (lacking phosphorylation sites for ERK1/2 and MAPKAPK-2) in HeLa cells. Collectively, our data suggest that compared with Ca2+-mediated 5-LO product formation, enzyme activation involving 5-LO phosphorylation events specifically and strongly alters the susceptibility of 5-LO toward nonredox-type inhibitors in intact cells. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264409 |
Ser664 |
QLPYYYLsPDRIPNS |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 12670876 |
Intriguingly, a significant difference in the potency of nonredox-type inhibitors (but not of BWA4C) was determined between wild-type 5-LO and the mutant S271A/S663A-5-LO (lacking phosphorylation sites for ERK1/2 and MAPKAPK-2) in HeLa cells. Collectively, our data suggest that compared with Ca2+-mediated 5-LO product formation, enzyme activation involving 5-LO phosphorylation events specifically and strongly alters the susceptibility of 5-LO toward nonredox-type inhibitors in intact cells. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
ERK1/2 | up-regulates activity
phosphorylation
|
ALOX5 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264443 |
Ser272 |
CSLERQLsLEQEVQQ |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 12670876 |
Intriguingly, a significant difference in the potency of nonredox-type inhibitors (but not of BWA4C) was determined between wild-type 5-LO and the mutant S271A/S663A-5-LO (lacking phosphorylation sites for ERK1/2 and MAPKAPK-2) in HeLa cells. Collectively, our data suggest that compared with Ca2+-mediated 5-LO product formation, enzyme activation involving 5-LO phosphorylation events specifically and strongly alters the susceptibility of 5-LO toward nonredox-type inhibitors in intact cells. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264442 |
Ser664 |
QLPYYYLsPDRIPNS |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 12670876 |
Intriguingly, a significant difference in the potency of nonredox-type inhibitors (but not of BWA4C) was determined between wild-type 5-LO and the mutant S271A/S663A-5-LO (lacking phosphorylation sites for ERK1/2 and MAPKAPK-2) in HeLa cells. Collectively, our data suggest that compared with Ca2+-mediated 5-LO product formation, enzyme activation involving 5-LO phosphorylation events specifically and strongly alters the susceptibility of 5-LO toward nonredox-type inhibitors in intact cells. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
MAPKAPK2 | up-regulates activity
phosphorylation
|
ALOX5 |
0.52 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250143 |
Ser272 |
CSLERQLsLEQEVQQ |
in vitro |
|
| pmid |
sentence |
| 11844797 |
Arachidonic acid promotes phosphorylation of 5-lipoxygenase at Ser-271 by MAPK-activated protein kinase 2 (MK2). when stimulated with only exogenous arachidonic acid, activity for the S271A mutant was significantly lower as compared with wild type 5-LO. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
CAMK2A | up-regulates activity
phosphorylation
|
ALOX5 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264408 |
Ser272 |
CSLERQLsLEQEVQQ |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 18978352 |
Phosphorylation of serine 271 on 5-lipoxygenase and its role in nuclear export|We report here that 5-LO is constitutively phosphorylated on Ser-271 in transfected NIH 3T3 cells. This residue is nested in a classical nuclear export sequence, and phosphorylated Ser-271 5-LO was exclusively found in the nucleus by immunofluorescence and by fractionation techniques|Nuclear export of 5-LO can also be induced by KN-93, an inhibitor of Ca2+/calmodulin-dependent kinase II, and the effects of SB 203,580 plus KN-93 are additive. Finally, HeLa cells, which lack nuclear 5-LO, also lack constitutive phosphorylation of Ser-271. Taken together, these results indicate that the phosphorylation of Ser-271 serves to inhibit the nuclear export of 5-LO. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAPK3 | up-regulates activity
phosphorylation
|
ALOX5 |
0.339 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264441 |
Ser272 |
CSLERQLsLEQEVQQ |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 12670876 |
Intriguingly, a significant difference in the potency of nonredox-type inhibitors (but not of BWA4C) was determined between wild-type 5-LO and the mutant S271A/S663A-5-LO (lacking phosphorylation sites for ERK1/2 and MAPKAPK-2) in HeLa cells. Collectively, our data suggest that compared with Ca2+-mediated 5-LO product formation, enzyme activation involving 5-LO phosphorylation events specifically and strongly alters the susceptibility of 5-LO toward nonredox-type inhibitors in intact cells. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264440 |
Ser664 |
QLPYYYLsPDRIPNS |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 12670876 |
Intriguingly, a significant difference in the potency of nonredox-type inhibitors (but not of BWA4C) was determined between wild-type 5-LO and the mutant S271A/S663A-5-LO (lacking phosphorylation sites for ERK1/2 and MAPKAPK-2) in HeLa cells. Collectively, our data suggest that compared with Ca2+-mediated 5-LO product formation, enzyme activation involving 5-LO phosphorylation events specifically and strongly alters the susceptibility of 5-LO toward nonredox-type inhibitors in intact cells. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
PRKACA | down-regulates activity 
phosphorylation
|
ALOX5 |
0.342 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264410 |
Ser524 |
GMRGRKSsGFPKSVK |
in vitro |
|
| pmid |
sentence |
| 15280375 |
These results indicate that PKA phosphorylates 5-LO on Ser-523, which inhibits the catalytic activity of 5-LO and reduces cellular LT generation. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
Gbeta | up-regulates activity
phosphorylation
|
ALOX5 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-270088 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 12670876 |
Intriguingly, a significant difference in the potency of nonredox-type inhibitors (but not of BWA4C) was determined between wild-type 5-LO and the mutant S271A/S663A-5-LO (lacking phosphorylation sites for ERK1/2 and MAPKAPK-2) in HeLa cells. Collectively, our data suggest that compared with Ca2+-mediated 5-LO product formation, enzyme activation involving 5-LO phosphorylation events specifically and strongly alters the susceptibility of 5-LO toward nonredox-type inhibitors in intact cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
zileuton | down-regulates activity
chemical inhibition
|
ALOX5 |
0.8 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-258363 |
|
|
Rattus norvegicus |
|
| pmid |
sentence |
| 1848634 |
5-lipoxygenase inhibitory activity of zileuton. |
|
| Publications: |
1 |
Organism: |
Rattus Norvegicus |
| + |
SP1 | up-regulates quantity by expression 
transcriptional regulation
|
ALOX5 |
0.255 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254032 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19781662 |
The 5-LO promoter possesses a unique GC-rich region which contains consensus sequences for the transcription factors Sp1 and Egr-1 (GC-boxes) which are important for basal transcriptional activity |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MBD2 | down-regulates quantity by repression 
transcriptional regulation
|
ALOX5 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254026 |
|
|
Homo sapiens |
U-937 Cell |
| pmid |
sentence |
| 19781662 |
Human 5-lipoxygenase (5-LO) is the key enzyme in the formation of inflammatory leukotrienes. 5-LO gene expression is mainly restricted to B cells and cells of myeloid origin. It is known that basal 5-lipoxygenase promoter activity is regulated by DNA methylation.|Using ChIP assays, we found that the methyl-DNA binding proteins MBD1, MBD2 and MeCP2 bind to the methylated 5-LO core promoter in U937 cells. Knock down of each of the MBDs upregulates 5-LO mRNA expression in U937 cells indicating that these proteins are involved in silencing of the 5-LO gene. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
ALOX5 | up-regulates
chemical modification
|
leukotriene A4 |
0.8 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-113198 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 11751058 |
5-lipoxygenase catalyzes the production of leukotriene (lt) a4, from 5- hydroperoxyeicosatetraenoic acid (5-hpete) as well as the nitial oxidation of arachidonic acid to this hydroperoxy in-termediate |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MBD1 | down-regulates quantity by repression
transcriptional regulation
|
ALOX5 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254030 |
|
|
Homo sapiens |
U-937 Cell |
| pmid |
sentence |
| 19781662 |
Human 5-lipoxygenase (5-LO) is the key enzyme in the formation of inflammatory leukotrienes. 5-LO gene expression is mainly restricted to B cells and cells of myeloid origin. It is known that basal 5-lipoxygenase promoter activity is regulated by DNA methylation.|Using ChIP assays, we found that the methyl-DNA binding proteins MBD1, MBD2 and MeCP2 bind to the methylated 5-LO core promoter in U937 cells. Knock down of each of the MBDs upregulates 5-LO mRNA expression in U937 cells indicating that these proteins are involved in silencing of the 5-LO gene. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MECP2 | down-regulates quantity by repression
transcriptional regulation
|
ALOX5 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254062 |
|
|
Homo sapiens |
U-937 Cell |
| pmid |
sentence |
| 19781662 |
Human 5-lipoxygenase (5-LO) is the key enzyme in the formation of inflammatory leukotrienes. 5-LO gene expression is mainly restricted to B cells and cells of myeloid origin. It is known that basal 5-lipoxygenase promoter activity is regulated by DNA methylation.|Using ChIP assays, we found that the methyl-DNA binding proteins MBD1, MBD2 and MeCP2 bind to the methylated 5-LO core promoter in U937 cells. Knock down of each of the MBDs upregulates 5-LO mRNA expression in U937 cells indicating that these proteins are involved in silencing of the 5-LO gene. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
ALOX5
- 
- 