| + |
MAPK3 | up-regulates quantity by stabilization 
phosphorylation
|
DEPTOR |
0.281 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277587 |
Ser235 |
MELLNEKsPSSQETH |
in vitro |
|
| pmid |
sentence |
| 35216969 |
Screening the DEPTOR interactome identified that the association of USP-7 deubiquitinase with DEPTOR was dependent upon S235 phosphorylation. Inhibition of USP-7 activity resulted in DEPTOR polyubiquitination and degradation. A scansite search suggested that ERK1 may be responsible for S235 phosphorylation, which was confirmed through the use of inhibitors, ERK1 knockdown, and an in vitro kinase assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
CSNK1A1 | down-regulates 
phosphorylation
|
DEPTOR |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-176871 |
Ser286 |
SSMSSCGsSGYFSSS |
Homo sapiens |
|
| pmid |
sentence |
| 22017877 |
Phosphorylation of all three serine residues in the deptor degron (ser286, ser287, and ser291) is necessary for - and directly mediates - the interaction with _trcp. ck1 phosphorylated the degron of deptor, as shown by western blotting with the phospho-specific antibody (fig. S3e-f). In contrast, mtor alone was unable to induce phosphorylation of deptor on ser286, ser287, and ser291. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-176875 |
Ser287 |
SMSSCGSsGYFSSSP |
Homo sapiens |
|
| pmid |
sentence |
| 22017877 |
Phosphorylation of all three serine residues in the deptor degron (ser286, ser287, and ser291) is necessary for - and directly mediates - the interaction with _trcp. ck1 phosphorylated the degron of deptor, as shown by western blotting with the phospho-specific antibody (fig. S3e-f). In contrast, mtor alone was unable to induce phosphorylation of deptor on ser286, ser287, and ser291. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-176879 |
Ser291 |
CGSSGYFsSSPTLSS |
Homo sapiens |
|
| pmid |
sentence |
| 22017877 |
Phosphorylation of all three serine residues in the deptor degron (ser286, ser287, and ser291) is necessary for - and directly mediates - the interaction with _trcp. ck1 phosphorylated the degron of deptor, as shown by western blotting with the phospho-specific antibody (fig. S3e-f). In contrast, mtor alone was unable to induce phosphorylation of deptor on ser286, ser287, and ser291. |
|
| Publications: |
3 |
Organism: |
Homo Sapiens |
| + |
RPS6K | down-regulates
phosphorylation
|
DEPTOR |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-252797 |
Ser286 |
SSMSSCGsSGYFSSS |
Homo sapiens |
|
| pmid |
sentence |
| 22017877 |
We found that deptor was rapidly phosphorylated on three serines in a conserved degron, facilitating binding and ubiquitylation by the f box protein _trcp, with consequent proteasomal degradation of deptor. Phosphorylation of the _trcp degron in deptor is executed by ck1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-252798 |
Ser287 |
SMSSCGSsGYFSSSP |
Homo sapiens |
|
| pmid |
sentence |
| 22017877 |
We found that deptor was rapidly phosphorylated on three serines in a conserved degron, facilitating binding and ubiquitylation by the f box protein _trcp, with consequent proteasomal degradation of deptor. Phosphorylation of the _trcp degron in deptor is executed by ck1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-252799 |
Ser291 |
CGSSGYFsSSPTLSS |
Homo sapiens |
|
| pmid |
sentence |
| 22017877 |
We found that deptor was rapidly phosphorylated on three serines in a conserved degron, facilitating binding and ubiquitylation by the f box protein _trcp, with consequent proteasomal degradation of deptor. Phosphorylation of the _trcp degron in deptor is executed by ck1 |
|
| Publications: |
3 |
Organism: |
Homo Sapiens |
| + |
RPS6KB1 | down-regulates
phosphorylation
|
DEPTOR |
0.659 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-176858 |
Ser286 |
SSMSSCGsSGYFSSS |
Homo sapiens |
|
| pmid |
sentence |
| 22017876 |
Deptor is phosphorylated by s6k1 and rsk1 on the degron serine residues upon serum stimulation s6k1/rsk1 and _trcp are required for ubiquitination and degradation of endogenous deptor upon mitogen stimulation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-176862 |
Ser287 |
SMSSCGSsGYFSSSP |
Homo sapiens |
|
| pmid |
sentence |
| 22017876 |
Deptor is phosphorylated by s6k1 and rsk1 on the degron serine residues upon serum stimulation s6k1/rsk1 and _trcp are required for ubiquitination and degradation of endogenous deptor upon mitogen stimulation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-176866 |
Ser291 |
CGSSGYFsSSPTLSS |
Homo sapiens |
|
| pmid |
sentence |
| 22017876 |
Deptor is phosphorylated by s6k1 and rsk1 on the degron serine residues upon serum stimulation s6k1/rsk1 and _trcp are required for ubiquitination and degradation of endogenous deptor upon mitogen stimulation. |
|
| Publications: |
3 |
Organism: |
Homo Sapiens |
| + |
RPS6KA1 | down-regulates
phosphorylation
|
DEPTOR |
0.493 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-176883 |
Ser286 |
SSMSSCGsSGYFSSS |
Homo sapiens |
|
| pmid |
sentence |
| 22017877 |
We found that deptor was rapidly phosphorylated on three serines in a conserved degron, facilitating binding and ubiquitylation by the f box protein _trcp, with consequent proteasomal degradation of deptor. Phosphorylation of the _trcp degron in deptor is executed by ck1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-176887 |
Ser287 |
SMSSCGSsGYFSSSP |
Homo sapiens |
|
| pmid |
sentence |
| 22017877 |
We found that deptor was rapidly phosphorylated on three serines in a conserved degron, facilitating binding and ubiquitylation by the f box protein _trcp, with consequent proteasomal degradation of deptor. Phosphorylation of the _trcp degron in deptor is executed by ck1 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-176891 |
Ser291 |
CGSSGYFsSSPTLSS |
Homo sapiens |
|
| pmid |
sentence |
| 22017877 |
We found that deptor was rapidly phosphorylated on three serines in a conserved degron, facilitating binding and ubiquitylation by the f box protein _trcp, with consequent proteasomal degradation of deptor. Phosphorylation of the _trcp degron in deptor is executed by ck1 |
|
| Publications: |
3 |
Organism: |
Homo Sapiens |
| + |
CHKA | down-regulates quantity by destabilization
phosphorylation
|
DEPTOR |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279604 |
Ser286 |
SSMSSCGsSGYFSSS |
Homo sapiens |
|
| pmid |
sentence |
| 22017875 |
DEPTOR phosphorylation by mTOR in response to growth signals, and in collaboration with casein kinase I (CKI), generates a phosphodegron that binds βTrCP.|These data suggests that CKI overexpression may overcome a requirement for phosphorylation at the major mTOR sites in DEPTOR for formation of the degron and are consistent with our finding that CKI can phosphorylate S286 and S287 in DEPTOR in vitro in the absence of mTOR. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279605 |
Ser287 |
SMSSCGSsGYFSSSP |
Homo sapiens |
|
| pmid |
sentence |
| 22017875 |
These data suggests that CKI overexpression may overcome a requirement for phosphorylation at the major mTOR sites in DEPTOR for formation of the degron and are consistent with our finding that CKI can phosphorylate S286 and S287 in DEPTOR in vitro in the absence of mTOR. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
MTOR | down-regulates
phosphorylation
|
DEPTOR |
0.754 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-176849 |
Ser293 |
SSGYFSSsPTLSSSP |
Homo sapiens |
|
| pmid |
sentence |
| 22017875 |
Our data reveal critical roles for mtor itself as well as cki in generating a degron in deptor that is recognized by _-trcp, and promotes deptor turnover by the proteasome. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-176853 |
Ser299 |
SSPTLSSsPPVLCNP |
Homo sapiens |
|
| pmid |
sentence |
| 22017875 |
Our data reveal critical roles for mtor itself as well as cki in generating a degron in deptor that is recognized by _-trcp, and promotes deptor turnover by the proteasome. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
SYK | down-regulates activity
phosphorylation
|
DEPTOR |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277572 |
Tyr289 |
SSCGSSGyFSSSPTL |
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 34634301 |
We found that tyrosine (Tyr) 289 phosphorylation of DEPTOR impairs its interaction with mTOR, leading to increased mTOR activation. Using proximity biotinylation assays, we identified SYK (spleen tyrosine kinase) as a kinase involved in DEPTOR Tyr 289 phosphorylation in an ephrin (erythropoietin-producing hepatocellular carcinoma) receptor-dependent manner. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DEPTOR | form complex 
binding
|
mTORC1 |
0.726 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-205600 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 25628925 |
Depending on their binding partners and sensitivities to rapamycin, mtor resides in at least two distinct complexes, termed mtor complex 1 (mtorc1, containing raptor, fkbp12, pras40 and mlst8) and mtor complex 2 (mtorc2, containing rictor, sin1, protor and mlst8) |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DEPTOR | form complex
binding
|
mTORC2 |
0.723 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-205603 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 25628925 |
Depending on their binding partners and sensitivities to rapamycin, mtor resides in at least two distinct complexes, termed mtor complex 1 (mtorc1, containing raptor, fkbp12, pras40 and mlst8) and mtor complex 2 (mtorc2, containing rictor, sin1, protor and mlst8) |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DEPTOR | down-regulates activity
binding
|
mTORC2 |
0.723 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-251659 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 19446321 |
DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for their survival |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
RNF7 | down-regulates activity
ubiquitination
|
DEPTOR |
0.299 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271449 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23136067 |
SAG (Sensitive to Apoptosis Gene), also known as RBX2 (RING box protein 2), ROC2 (Regulator of Cullins 2), or RNF7 (RING Finger Protein 7), was originally cloned in our laboratory as a redox inducible antioxidant protein and later characterized as the second member of the RBX/ROC RING component of the SCF (SKP1-CUL-F-box Proteins) E3 ubiquitin ligase. by forming a complex with other components of the SCF E3 ligase, SAG promotes ubiquitination and degradation of a number of protein substrates, including c-JUN, DEPTOR, HIF-1α, IκBα, NF1, NOXA, p27, and procaspase-3, thus regulating various signaling pathways and biological processes. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DEPTOR | down-regulates activity
binding
|
mTORC1 |
0.726 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-251658 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 19446321 |
DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for their survival |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
USP7 | up-regulates quantity by stabilization
deubiquitination
|
DEPTOR |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277588 |
|
|
in vitro |
|
| pmid |
sentence |
| 35216969 |
Screening the DEPTOR interactome identified that the association of USP-7 deubiquitinase with DEPTOR was dependent upon S235 phosphorylation. Inhibition of USP-7 activity resulted in DEPTOR polyubiquitination and degradation. A scansite search suggested that ERK1 may be responsible for S235 phosphorylation, which was confirmed through the use of inhibitors, ERK1 knockdown, and an in vitro kinase assay. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
DEPTOR | down-regulates activity
binding
|
MTOR |
0.754 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-251657 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 19446321 |
DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for their survival |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
DEPTOR
- 
- 