| + |
NLK | down-regulates 
phosphorylation
|
LEF1 |
0.75 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-97808 |
Ser166 |
TYSDEHFsPGSHPSH |
Homo sapiens |
|
| pmid |
sentence |
| 12556497 |
Regulation of lymphoid enhancer factor 1/t-cell factor by mitogen-activated protein kinase-related nemo-like kinase-dependent phosphorylation in wnt/beta-catenin signaling.Nlk phosphorylates lef-1/tcf on two serine/threonine residues located in its central region. Mutation of both residues to alanine enhanced lef-1 transcriptional activity and rendered it resistant to inhibition by nlk. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-97812 |
Thr155 |
SHAVHPLtPLITYSD |
Homo sapiens |
|
| pmid |
sentence |
| 12556497 |
Regulation of lymphoid enhancer factor 1/t-cell factor by mitogen-activated protein kinase-related nemo-like kinase-dependent phosphorylation in wnt/beta-catenin signaling.Nlk phosphorylates lef-1/tcf on two serine/threonine residues located in its central region. Mutation of both residues to alanine enhanced lef-1 transcriptional activity and rendered it resistant to inhibition by nlk. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
LEF1 | up-regulates quantity by expression 
binding
|
ALX4 |
0.457 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254547 |
|
|
Mus musculus |
Mesenchymal Stromal Cell |
| pmid |
sentence |
| 11696550 |
Alx4 Stably Interacts with LEF-1. LEF-1 enhances Alx4 binding to DNA, suggesting that both interaction with LEF-1 and DNA binding are required to mediate its effects on the N-CAM promoter. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
LEF1 | down-regulates quantity by repression 
transcriptional regulation
|
IL4 |
0.357 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254504 |
|
|
Homo sapiens |
T-lymphocyte |
| pmid |
sentence |
| 18579517 |
We identified a high affinity LEF-1-binding site in the negative regulatory element of the IL-4 promoter. Knockdown LEF-1 expression by LEF-1-specific small interfering RNA resulted in an increase in the IL-4 mRNA expression |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CTNNB1 | up-regulates activity
binding
|
LEF1 |
0.914 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-169632 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21078818 |
Phosphorylated lrp5/6 leads to inhibition of the so-called beta-catenin destruction complex (which includes axin, gsk3, dvl, ck1, and the tumor suppressor adenomatous polyposis coli), resulting in the stabilization and translocation of beta-catenin in the nucleus, where it activates target genes through binding to tcf/lef transcription factors. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-199378 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23151663 |
Upon wnt activation, cytoplasmic beta-catenin is stabilized and enters the nucleus, where it associates with transcription factors, notably tcf (t cell factor) and lef (lymphoid enhancer-binding factor), to regulate the transcription of target genes. Thus beta-catenin regulates gene expression by direct interaction with transcription factors such as lef-1, providing a molecular mechanism for the transmission of signals, from cell-adhesion components or wnt protein to the nucleus. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-134219 |
|
|
Homo sapiens |
T-lymphocyte |
| pmid |
sentence |
| 15735151 |
Activated dvl binds and inhibits the phosphorylation of beta catenin by gsk3beta/alfa, blocking beta catenin degradation), so that beta catenin accumulates and translocates to the nucleus, where it interacts with the t cell specific factor (tcf)/lymphoid enhancer binding factor 1 (lef-1) transcription factor and induces the transcription of target genes such as c-jun, c-myc, and cyclin d1 |
|
| Publications: |
3 |
Organism: |
Homo Sapiens |
| Pathways: | Colorectal Carcinoma, Hepatocellular Tumor, Wnt in cancer, WNT Signaling, WNT/FLT3, WNT Signaling and Myogenesis |
| + |
NLK | down-regulates quantity
phosphorylation
|
LEF1 |
0.75 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271596 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 16714285 |
NLK Augments the Ubiquitylation Activity of NARF against TCF/LEF. ctivation of NLK induced by unknown ligands leads to the phosphorylation of TCF/LEF. NARF then acts on TCF/LEF as an E3 ubiquitin-ligase and, together with E1 and E2 ubiquitylation enzymes, catalyze the ubiquitylation of TCF/LEF. Finally, ubiquitylated TCF/LEF is degraded by the 26 S proteasome. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
LEF1 | up-regulates quantity by expression 
transcriptional regulation
|
MYF5 |
0.255 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-149170 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 16936075 |
Furthermore, we show that direct activation is mediated by binding of the tcf-lef/ - catenin complex to the myf5 epaxial enhancer and to a newly identified element upstream of this enhancer. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Tissue: |
Muscle |
| Pathways: | WNT Signaling and Myogenesis |
| + |
CSNK2A1 | up-regulates
phosphorylation
|
LEF1 |
0.308 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-23958 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 2861485 |
Here, we identify ck1 and ck2 as major kinases that directly bind to and phosphorylate lef-1 inducing distinct, kinase-specific changes in the lef-1/dna complex.CK1-dependent phosphorylation inhibits, whereas ck2 activates lef-1/beta-catenin transcriptional activity in reporter gene assays. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-134500 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 15747065 |
Here, we identify ck1 and ck2 as major kinases that directly bind to and phosphorylate lef-1 inducing distinct, kinase-specific changes in the lef-1/dna complex. Ck1-dependent phosphorylation inhibits, whereas ck2 activates lef-1/beta-catenin transcriptional activity in reporter gene assays. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-187209 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19623618 |
Here, we identify ck1 and ck2 as major kinases that directly bind to and phosphorylate lef-1 inducing distinct, kinase-specific changes in the lef-1/dna complex.CK1-dependent phosphorylation inhibits, whereas ck2 activates lef-1/beta-catenin transcriptional activity in reporter gene assays. |
|
| Publications: |
3 |
Organism: |
Homo Sapiens |
| + |
RNF138 | down-regulates quantity by destabilization
polyubiquitination
|
LEF1 |
0.311 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-271595 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 16714285 |
Here, we show that NARF induces the ubiquitylation of TCF/LEF in vitro and in vivo, and functions as an E3 ubiquitin-ligase that specifically cooperates with the E2 conjugating enzyme E2-25K. We found that NLK augmented NARF binding and ubiquitylation of TCF/LEF, and this required NLK kinase activity. The ubiquitylated TCF/LEF was subsequently degraded by the proteasome. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
LEF1 | up-regulates quantity by expression
transcriptional regulation
|
CEBPA |
0.356 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254551 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19620402 |
We have identified LEF-1 as a decisive transcription factor in granulopoiesis controlling proliferation and granulocytic differentiation by direct activation of its target gene, C/EBPalpha. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
LEF1 | down-regulates quantity by repression
transcriptional regulation
|
DSG4 |
0.292 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254183 |
|
|
Homo sapiens |
HaCaT Cell |
| pmid |
sentence |
| 19683850 |
we studied the transcriptional regulation of DSG4 by transcription factors/pathways that are known regulators of hair keratin or KAP expression. We show that HOXC13, LEF1 and FOXN1 repress DSG4 transcription and provide in vitro and in vivo evidence correlating the Notch pathway with the activation and/or maintenance of DSG4 expression in the hair follicle. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
LEF1 | up-regulates activity
transcriptional regulation
|
NCAM1 |
0.279 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254549 |
|
|
Mus musculus |
Mesenchymal Stromal Cell |
| pmid |
sentence |
| 11696550 |
Consistent with our observation that expression of exogenous LEF-1 causes transactivation of the N-CAM promoter, a recent study demonstrated that noggin-dependent induction of LEF-1 coincidentally increased N-CAM expression (50). Ectopic noggin added to skin cultures up-regulates LEF-1 expression and stimulates hair induction. Based on promoter assays and EMSAs, our results further support the notion that N-CAM is a direct target of LEF-1. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
LEF1 | up-regulates quantity by expression
transcriptional regulation
|
PITX2 |
0.708 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-188730 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19850024 |
These results suggest that wnt/lef1 signaling regulates epaxial myogenesis via pitx2 but that this link is uncoupled in other regions of the body, emphasizing the unique molecular networks that control the development of various muscles in vertebrates. The pitx2 promoter contains tcf/lef binding sites and expression can be induced by licl, which activates the canonical wnt signaling pathway |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Tissue: |
Muscle |
| Pathways: | WNT Signaling, WNT Signaling and Myogenesis |
| + |
LEF1 | up-regulates quantity by expression
transcriptional regulation
|
ELANE |
0.259 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254550 |
|
|
Homo sapiens |
Bone Marrow Cell |
| pmid |
sentence |
| 14594802 |
We find that LEF-1 and CBFalpha co-activate ELA2 expression. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TLE1 | down-regulates activity
binding
|
LEF1 |
0.676 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260109 |
|
|
in vitro |
|
| pmid |
sentence |
| 19460168 |
Our data shows that Groucho/TLE repression requires two sites of interaction in LEF-1 and that a central, conserved amino acid sequence within the primary region (F S/T/P/xx y I/L/V) is critical. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
PAX3 | up-regulates activity
binding
|
LEF1 |
0.438 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-162100 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20006729 |
Pax3 binds to lef1 pax3 activity may directly effect the expression of factors regulated by signal transduction pathways dependent on lef1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | WNT Signaling and Myogenesis |
| + |
LEF1 | up-regulates quantity by expression
transcriptional regulation
|
CLDN2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254120 |
|
|
Mus musculus |
C57MG Cell |
| pmid |
sentence |
| 14751232 |
Activity of the claudin-2 promoter was elevated in mouse mammary epithelial C57 cells expressing Wnt-1. LEF-1, a nuclear effector of the Wnt signaling pathway which is involved in the regulation of cell differentiation and polarization, was found to bind directly to the claudin-2 promoter as revealed by electrophoretic mobility shift assays. Expression of LEF-1 and beta-catenin both enhanced claudin-2 promoter activity. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| + |
LEF1 | up-regulates
|
Differentiation |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-229770 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17081971 |
The interaction of beta-catenin with the N terminus of tcf/lef transiently converts it into an activator, translating the Wnt signal into the transient transcription of Tcf target genes. The Wnt pathway has distinct transcriptional outputs, which are determined by the identity of the responding cell, and range from cell proliferation and survival to the terminal differentiation of postmitotic cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | WNT Signaling |
| + |
SMAD4 | up-regulates activity
|
LEF1 |
0.677 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-229311 |
|
|
Homo sapiens |
Hep-G2 Cell |
| pmid |
sentence |
| 10890911 |
Coexpression of smad2 and smad4, smad3 alone, or smad3 and smad4 resulted in strong enhancement of lef1-dependent transcriptional activity |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Colorectal Carcinoma, Hepatocellular Tumor |
| + |
LEF1 | up-regulates quantity by expression
transcriptional regulation
|
MYC |
0.62 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-245351 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19653274 |
Expression of Lef-1 FL, but not the newly identified Lef-1 Deltaexon VI, induced the expression of the cell cycle regulating proteins c-myc and cyclin D1 in cooperation with beta-catenin and it enhanced cell proliferation |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Colorectal Carcinoma, Hepatocellular Tumor, Wnt in cancer, WNT Signaling, WNT/FLT3 |
| + |
LEF1 | up-regulates
|
Proliferation |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-229764 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17081971 |
The interaction of beta-catenin with the N terminus of tcf/lef transiently converts it into an activator, translating the Wnt signal into the transient transcription of Tcf target genes. The Wnt pathway has distinct transcriptional outputs, which are determined by the identity of the responding cell, and range from cell proliferation and survival to the terminal differentiation of postmitotic cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Colorectal Carcinoma, Hepatocellular Tumor, Wnt in cancer, WNT Signaling, WNT/FLT3 |
| + |
LEF1 | up-regulates quantity by expression
transcriptional regulation
|
OCA2 |
0.256 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-254555 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 22234890 |
the SNP rs12913832 has strong statistical association with human pigmentation. It is located within an intron of the nonpigment gene HERC2, 21 kb upstream of the pigment gene OCA2, and the region surrounding rs12913832 is highly conserved among animal species.In darkly pigmented human melanocytes carrying the rs12913832 T-allele, we detected binding of the transcription factors HLTF, LEF1, and MITF to the HERC2 rs12913832 enhancer, and a long-range chromatin loop between this enhancer and the OCA2 promoter that leads to elevated OCA2 expression. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SMAD3/SMAD4 | up-regulates
transcriptional regulation
|
LEF1 |
0.604 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-217169 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 10890911 |
Coexpression of smad2 and smad4, smad3 alone, or smad3 and smad4 resulted in strong enhancement of lef1-dependent transcriptional activity |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Hepatocellular Tumor |
| + |
LEF1 | up-regulates quantity by expression
transcriptional regulation
|
CCND1 |
0.584 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-256281 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19653274 |
Expression of Lef-1 FL, but not the newly identified Lef-1 Deltaexon VI, induced the expression of the cell cycle regulating proteins c-myc and cyclin D1 in cooperation with beta-catenin and it enhanced cell proliferation |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CSNK1E | down-regulates
phosphorylation
|
LEF1 |
0.271 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-134497 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 15747065 |
Here, we identify ck1 and ck2 as major kinases that directly bind to and phosphorylate lef-1 inducing distinct, kinase-specific changes in the lef-1/dna complex.CK1-dependent phosphorylation inhibits, whereas ck2 activates lef-1/beta-catenin transcriptional activity in reporter gene assays. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
LEF1 | up-regulates
|
Survival |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-229767 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17081971 |
The interaction of beta-catenin with the N terminus of tcf/lef transiently converts it into an activator, translating the Wnt signal into the transient transcription of Tcf target genes. The Wnt pathway has distinct transcriptional outputs, which are determined by the identity of the responding cell, and range from cell proliferation and survival to the terminal differentiation of postmitotic cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Colorectal Carcinoma, Hepatocellular Tumor, WNT Signaling |
| + |
SMAD2 | up-regulates activity
|
LEF1 |
0.463 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-78988 |
|
|
Homo sapiens |
Hep-G2 Cell |
| pmid |
sentence |
| 10890911 |
Coexpression of smad2 and smad4, smad3 alone, or smad3 and smad4 resulted in strong enhancement of lef1-dependent transcriptional activity |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Hepatocellular Tumor |
| + |
SMAD3 | up-regulates activity
|
LEF1 |
0.53 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-229308 |
|
|
Homo sapiens |
Hep-G2 Cell |
| pmid |
sentence |
| 10890911 |
Coexpression of smad2 and smad4, smad3 alone, or smad3 and smad4 resulted in strong enhancement of lef1-dependent transcriptional activity |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Colorectal Carcinoma, Hepatocellular Tumor |
| + |
CSNK1D | down-regulates
phosphorylation
|
LEF1 |
0.253 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-134494 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 15747065 |
Cki_/_ binds and phosphorylates lef-1, and this phosphorylation disrupts lef-1_-catenin interaction |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TLE2 | down-regulates
binding
|
LEF1 |
0.622 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-185736 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19460168 |
Mapping studies reveal that groucho/tle binds two regions in lef-1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
LEF1 | up-regulates quantity by expression
transcriptional regulation
|
FST |
0.284 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-251722 |
|
|
Mus musculus |
C2C12 Cell |
| pmid |
sentence |
| 24344199 |
We further demonstrate that Fst is a direct target of the WNT/β-catenin pathway. Activation and inactivation of β-catenin induced and inhibited Fst expression, respectively, in both C2C12 cells and mouse embryos. Specific TCF/LEF1 binding sites within the promoter and intron 1 region of the Fst gene were required for RSPO2 and WNT/β-catenin-induced Fst expression. |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
3.0
LEF1
- 
- 