Relation Results

Summary

Name RNF8
Full Name E3 ubiquitin-protein ligase RNF8
Synonyms hRNF8, RING finger protein 8 | KIAA0646
Primary ID O76064
Links - -
Type protein
Relations 15
Function E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles

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Type: Score: Layout: SPV 
0.20.70.20.7570.20.20.2420.7490.3290.3020.20.4180.20.3380.2RNF8ACDDNA_repairH2AC11MDC1H1-2Ub:E2L3MBTL2UBE2NBCL10TPP1XRN2RAD18H2AXBLMHistone H2A

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▶Modifications Tables

Relations
Regulator
Mechanism
target
score ℹ
+ RNF8up-regulates quantity by stabilization img/direct-activation.png polyubiquitination ACD 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272722 Lys147 QDLDVQKkLYDCLEE Homo sapiens
pmid sentence
The Rnf8 RING-finger domain is essential for Tpp1 stability and retention at telomeres. Rnf8 physically interacts with Tpp1 to generate Ubc13-dependent Lys63 polyubiquitin chains that stabilize Tpp1 at telomeres. The conserved Tpp1 residue Lys233 is important for Rnf8-mediated Tpp1 ubiquitylation and localization to telomeres.
Publications: 1 Organism: Homo Sapiens
+ RNF8up-regulates img/indirect-activation.png DNA_repair 0.7
Identifier Residue Sequence Organism Cell Line
SIGNOR-266790 Homo sapiens
pmid sentence
L3MBTL2 links RNF8 and RNF168 in the DNA double strand break response. The protein kinase ATM phosphorylates L3MBTL2, which recruits it to the DNA lesion by promoting the interaction between MDC1 and L3MBTL2. L3MBTL2 is subsequently ubiquitinated by RNF8, which acts as a docking site for RNF168, thereby recruiting the ubiquitin ligase to the damage site. RNF168, in turn, ubiquitinates H2A-type histones to amplify the DNA damage response and recruit downstream DNA repair proteins for proper DSB signaling.
Publications: 1 Organism: Homo Sapiens
+ RNF8up-regulates img/direct-activation.png ubiquitination H2AC11 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-166174 Homo sapiens
pmid sentence
Rnf8 and ubc13 ubiquitylate h2a and h2ax, but other substrates probably exist.
Publications: 1 Organism: Homo Sapiens
+ MDC1up-regulates img/direct-activation.png relocalization RNF8 0.757
Identifier Residue Sequence Organism Cell Line
SIGNOR-179820 Homo sapiens
pmid sentence
Rnf8 relocalizes to dna damage sites via a phospho-dependent interaction with mdc1
Publications: 1 Organism: Homo Sapiens
+ RNF8down-regulates img/direct_inhibition.png polyubiquitination H1-2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272928 Homo sapiens MDA-MB-231 Cell
pmid sentence
ITCH interacts with and ubiquitinates linker histone H1.2 at K46. ITCH biochemically competes with RNF168 and RNF8 to polyubiquitinate histone H1.2.
Publications: 1 Organism: Homo Sapiens
+ Ub:E2up-regulates activity img/direct-activation.png ubiquitination RNF8 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-270987 Homo sapiens
pmid sentence
The ubiquitination process is mediated sequentially by three classes of enzymes consisting of a Ub-activating enzyme E1, a Ub-conjugating enzyme E2, and a Ub ligase E3. Ub is first activated by E1 in an adenosine 5′-triphosphate (ATP)-dependent manner t
Publications: 1 Organism: Homo Sapiens
+ RNF8up-regulates activity img/direct-activation.png ubiquitination L3MBTL2 0.242
Identifier Residue Sequence Organism Cell Line
SIGNOR-266787 Homo sapiens
pmid sentence
L3MBTL2 links RNF8 and RNF168 in the DNA double strand break response. The protein kinase ATM phosphorylates L3MBTL2, which recruits it to the DNA lesion by promoting the interaction between MDC1 and L3MBTL2. L3MBTL2 is subsequently ubiquitinated by RNF8, which acts as a docking site for RNF168, thereby recruiting the ubiquitin ligase to the damage site. RNF168, in turn, ubiquitinates H2A-type histones to amplify the DNA damage response and recruit downstream DNA repair proteins for proper DSB signaling.
Publications: 1 Organism: Homo Sapiens
+ RNF8up-regulates img/direct-activation.png binding UBE2N 0.749
Identifier Residue Sequence Organism Cell Line
SIGNOR-179823 Homo sapiens
pmid sentence
The rnf8 ring domain signals ubc13 to sites of damage, which is sufficient for dna damage signal transduction.
Publications: 1 Organism: Homo Sapiens
+ RNF8up-regulates quantity by stabilization img/direct-activation.png ubiquitination BCL10 0.329
Identifier Residue Sequence Organism Cell Line
SIGNOR-278778 Homo sapiens
pmid sentence
Phosphorylated and ubiquitinated BCL10 is stabilized on the damage sites through binding to and presenting UBC13 to RNF168.|We thus concluded that BCL10 is ubiquitinated mainly with K63-linked ubiquitination by RNF8.
Publications: 1 Organism: Homo Sapiens
+ RNF8up-regulates activity img/direct-activation.png ubiquitination TPP1 0.302
Identifier Residue Sequence Organism Cell Line
SIGNOR-278574 Homo sapiens
pmid sentence
Our data demonstrate that RNF8 directly ubiquitylates and stabilizes TPP1 at telomeres.|Taken together, these results suggest that RNF8 dependent K63 linked, but not K48 linked ubiquitin chain formation on TPP1 is required to promote TPP1 stability and function at telomeres.
Publications: 1 Organism: Homo Sapiens
+ RNF8up-regulates activity img/direct-activation.png ubiquitination XRN2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-277195 Homo sapiens
pmid sentence
Mechanistically, RNF8 interacts with XRN2, which is crucial for transcription termination and R-loop resolution. We report that RNF8 ubiquitylates XRN2 to facilitate its recruitment to R-loop-prone genomic loci and that RNF8 deficiency in BRCA1-mutant breast cancer cells decreases XRN2 occupancy at R-loop-prone sites, thereby promoting R-loop accumulation, transcription-replication collisions, excessive genomic instability, and cancer cell death.
Publications: 1 Organism: Homo Sapiens
+ RNF8up-regulates img/direct-activation.png binding RAD18 0.418
Identifier Residue Sequence Organism Cell Line
SIGNOR-185593 Homo sapiens
pmid sentence
Rnf8 depletion also significantly reduced the accumulation of rad18 to chromatin fraction after ir
Publications: 1 Organism: Homo Sapiens
+ RNF8up-regulates img/direct-activation.png ubiquitination H2AX 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-159309 Homo sapiens
pmid sentence
Rnf8 can ubiquitylate histone h2a and h2ax,
Publications: 1 Organism: Homo Sapiens
+ RNF8up-regulates activity img/direct-activation.png ubiquitination BLM 0.338
Identifier Residue Sequence Organism Cell Line
SIGNOR-272115 Homo sapiens U2-OS Cell
pmid sentence
Here, we demonstrate that the ubiquitin/SUMO-dependent DNA damage response (UbS-DDR), controlled by the E3 ligases RNF8/RNF168, triggers BLM recruitment to sites of replication fork stalling via ubiquitylation in the N-terminal region of BLM and subsequent BLM binding to the ubiquitin-interacting motifs of . 
Publications: 1 Organism: Homo Sapiens
+ RNF8up-regulates img/direct-activation.png ubiquitination Histone H2A 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-265313 Homo sapiens
pmid sentence
Rnf8 and ubc13 ubiquitylate h2a and h2ax, but other substrates probably exist.
Publications: 1 Organism: Homo Sapiens
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