| + |
ORC | up-regulates 
|
DNA_replication |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-267561 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 32808929 |
The dynamic nature of the human origin recognition complex revealed through five cryoEM structures|Genome replication is initiated from specific origin sites established by dynamic events. The Origin Recognition Complex (ORC) is necessary for orchestrating the initiation process by binding to origin DNA, recruiting CDC6, and assembling the MCM replicative helicase on DNA. Here we report five cryoEM structures of the human ORC (HsORC) that illustrate the native flexibility of the complex. |The very first step of this initiation process is accomplished by DNA association with the Origin Recognition Complex (ORC), a six-subunit protein that forms a partial ring around origin DNA |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DNA polymerase gamma | up-regulates
|
DNA_replication |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-265720 |
|
|
in vitro |
|
| pmid |
sentence |
| 19837034 |
DNA Pol gamma, in contrast to the many nuclear DNA polymerases (DNAPs) that have specialized functions, is solely responsible for DNA replication and repair in mitochondria. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
POLA1 | up-regulates
|
DNA_replication |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-261275 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19608746 |
Mcm10 is an essential eukaryotic protein required for the initiation and elongation phases of chromosomal replication. Specifically, Mcm10 is required for the association of several replication proteins, including DNA polymerase alpha (pol alpha), with chromatin. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MCM | up-regulates
|
DNA_replication |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-261678 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 19946136 |
The Mcm2-7 complex serves as the eukaryotic replicative helicase, the molecular motor that both unwinds duplex DNA and powers fork progression during DNA replication. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
DNA polymerase epsilon | up-regulates
|
DNA_replication |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-265514 |
|
|
Chlorocebus aethiops |
COS Cell |
| pmid |
sentence |
| 12930972 |
Processive DNA synthesis by DNA polymerases delta and epsilon requires the cellular replication factor C (RF‐C) and proliferating cell nuclear antigen (PCNA). |
|
| Publications: |
1 |
Organism: |
Chlorocebus Aethiops |
| + |
G1/S_transition | up-regulates
|
DNA_replication |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-245489 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21524151 |
In addition to the successive phosphorylation of pRb by active Cyclin/Cdk complexes, other factors can also impact upon S-phase entry Subsequently, the initiation of replication requires the formation of a pre-initiation complex (pre-IC) that is initiated by phosphorylation of Mcm2-7 by CyclinE/Cdk2 and DDK (Dbf4- and Drf1-dependent kinase) and recruitment of Cdc45 onto the chromatin (Figure 1). This recruitment is thought to be the critical step for the activation of the Mcm2-7 helicase activity and replication initiation. Finally, unwinding of the chromatin enables DNA-polymerase _ to initiate DNA synthesis and DNA-polymerase _ to continue replication |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition |
| + |
DNA polymerase delta | up-regulates
|
DNA_replication |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-265513 |
|
|
Chlorocebus aethiops |
COS Cell |
| pmid |
sentence |
| 12930972 |
Processive DNA synthesis by DNA polymerases delta and epsilon requires the cellular replication factor C (RF‐C) and proliferating cell nuclear antigen (PCNA). |
|
| Publications: |
1 |
Organism: |
Chlorocebus Aethiops |
| + |
DNA polymerase alpha:primase complex | up-regulates
|
DNA_replication |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-261344 |
|
|
|
|
| pmid |
sentence |
| 24043831 |
At the replication fork, primase is present in a constitutive complex with DNA polymerase α (Pol α), which extends the RNA primer with deoxynucleotides and makes the resulting RNA–DNA primer available to the leading- and lagging-strand polymerases, Pols ε and δ, for processive elongation |
|
| Publications: |
1 |
| + |
INO80 complex | up-regulates
|
DNA_replication |
0.7 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-270858 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 25016522 |
Here, we have systematically investigated the involvement of the catalytic subunit of the human INO80 complex during unchallenged replication and under replication stress by following the effects of its depletion on cell survival, S-phase checkpoint activation, the fate of individual replication forks, and the consequences of fork collapse. We report that INO80 was specifically needed for efficient replication elongation, while it was not required for initiation of replication. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
3.0
DNA_replication