| + |
BUB1 | down-regulates activity 
phosphorylation
|
CDC20 |
0.992 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250604 |
Ser153 |
NRLKVLYsQKATPGS |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 15525512 |
Bub1 directly phosphorylates Cdc20 in vitro and inhibits the ubiquitin ligase activity of APC/C(Cdc20) catalytically. A Cdc20 mutant with all six Bub1 phosphorylation sites removed is refractory to Bub1-mediated phosphorylation and inhibition in vitro. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250605 |
Ser161 |
QKATPGSsRKTCRYI |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 15525512 |
Bub1 directly phosphorylates Cdc20 in vitro and inhibits the ubiquitin ligase activity of APC/C(Cdc20) catalytically. A Cdc20 mutant with all six Bub1 phosphorylation sites removed is refractory to Bub1-mediated phosphorylation and inhibition in vitro. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250606 |
Ser41 |
EAAGPAPsPMRAANR |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 15525512 |
Bub1 directly phosphorylates Cdc20 in vitro and inhibits the ubiquitin ligase activity of APC/C(Cdc20) catalytically. A Cdc20 mutant with all six Bub1 phosphorylation sites removed is refractory to Bub1-mediated phosphorylation and inhibition in vitro. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250607 |
Ser72 |
SKVQTTPsKPGGDRY |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 15525512 |
Bub1 directly phosphorylates Cdc20 in vitro and inhibits the ubiquitin ligase activity of APC/C(Cdc20) catalytically. A Cdc20 mutant with all six Bub1 phosphorylation sites removed is refractory to Bub1-mediated phosphorylation and inhibition in vitro. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250608 |
Ser92 |
AAQMEVAsFLLSKEN |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 15525512 |
Bub1 directly phosphorylates Cdc20 in vitro and inhibits the ubiquitin ligase activity of APC/C(Cdc20) catalytically. A Cdc20 mutant with all six Bub1 phosphorylation sites removed is refractory to Bub1-mediated phosphorylation and inhibition in vitro. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-250609 |
Thr157 |
VLYSQKAtPGSSRKT |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 15525512 |
Bub1 directly phosphorylates Cdc20 in vitro and inhibits the ubiquitin ligase activity of APC/C(Cdc20) catalytically. A Cdc20 mutant with all six Bub1 phosphorylation sites removed is refractory to Bub1-mediated phosphorylation and inhibition in vitro. |
|
| Publications: |
6 |
Organism: |
Homo Sapiens |
| + |
PLK1 | down-regulates quantity by destabilization
phosphorylation
|
CDC20 |
0.976 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276493 |
Ser170 |
KTCRYIPsLPDRILD |
in vitro |
|
| pmid |
sentence |
| 23643811 |
Plk1 directly bound to Cdc20 and phosphorylates it on serine-170 located in CRY-box. Whereas wild-type Cdc20 was degraded according to progress cell cycle beyond mitosis, the phosphorylation-defective mutant, which serine-170 was changed into alanine, was not destroyed in early G1 phase. |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
CDK5RAP2 | down-regulates activity
binding
|
CDC20 |
0.316 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-260311 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 19282672 |
We show here that inhibition of CDK5RAP2 expression causes chromosome mis-segregation, fails to maintain the spindle checkpoint, and is associated with reduced expression of the spindle checkpoint proteins BUBR1 and MAD2 and an increase in chromatin-associated CDC20.|We found that the APC activator CDC20, but not others we exam-ined, was present in the CDK5RAP2 immunocomplex in HeLa cell extracts (Fig. 3A). CDK5RAP2 was detected in the CDC20 immunocomplex as well (Fig. 3B). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CDC20 | up-regulates activity 
binding
|
APC-c |
0.871 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272896 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 23287467 |
Here, we show that human REV1 undergoes proteosomal degradation mediated by the E3 ubiquitin ligase known as anaphase-promoting complex (APC). REV1 associates with APC. Overexpression of APC coactivator CDH1 or CDC20 promotes polyubiquitination and proteosomal degradation of REV1. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272880 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 23979597 |
We showed that PHF8 interacts with the CDC20-containing APC (APC(cdc20)) primarily during mitosis. we demonstrate that mutations of the LXPKXLF motif abrogate polyubiquitylation of PHF8 by the APC. APC substrates are typically cell cycle regulators, and consistent with this, the loss of PHF8 leads to prolonged G2 phase and defective mitosis. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-252014 |
|
|
|
|
| pmid |
sentence |
| 16896351 |
In addition to E2 enzymes, APC/C activity is also strictly dependent on one of several co-activator proteins that associate with APC/C during specific periods of the cell cycle. The best studied of these are Cdc20 and Cdh1 |
|
| Publications: |
3 |
Organism: |
Homo Sapiens, |
| + |
NEK2 | up-regulates activity
phosphorylation
|
CDC20 |
0.941 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278366 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20034488 |
In summary, we have demonstrated that Nek2 can associate with and phosphorylate Mad2 and Cdc20.|The results presented here support a model in which Nek2 modulates the functions of Mad2 and Cdc20 in the mitotic checkpoint and elevation of Nek2 levels may contribute to chromosome instability by interfering with the control of the checkpoint. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CDC20 | up-regulates activity
binding
|
UBE2S |
0.864 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-265082 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 19822757 |
Ube2S depends on the cell cycle-dependent association with the APC/C activators Cdc20 and Cdh1 for its activity |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
MAD2L2 | down-regulates activity
binding
|
CDC20 |
0.468 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264903 |
|
|
in vitro |
|
| pmid |
sentence |
| 11459826 |
The APC is activated in mitosis and G1 by CDC20 and CDH1, and inhibited by the checkpoint protein MAD2, a specific inhibitor of CDC20. We show here that a MAD2 homolog MAD2B also inhibits APC. MAD2B directly inhibits activation of APC by CDC20 and CDH1 |
|
| Publications: |
1 |
Organism: |
In Vitro |
| + |
CDK2 | down-regulates activity
phosphorylation
|
CDC20 |
0.692 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279322 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 26960431 |
Here we show that cyclin A2-Cdk2 binds and phosphorylates Cdc20 in interphase and this inhibits APC/C-Cdc20 activity.|Interphase APC/C-Cdc20 inhibition by cyclin A2-Cdk2 ensures efficient mitotic entry. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CDC20 | down-regulates quantity by destabilization
binding
|
PHF8 |
0.338 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272879 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 23979597 |
We showed that PHF8 interacts with the CDC20-containing APC (APC(cdc20)) primarily during mitosis. we demonstrate that mutations of the LXPKXLF motif abrogate polyubiquitylation of PHF8 by the APC. APC substrates are typically cell cycle regulators, and consistent with this, the loss of PHF8 leads to prolonged G2 phase and defective mitosis. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TBK1 | down-regulates activity
phosphorylation
|
CDC20 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279766 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 33310066 |
It was found that TBK1 phosphorylates both Cdc20 as well as Cdh1 (Figure 2F). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
TRiC | up-regulates quantity by stabilization
binding
|
CDC20 |
0.52 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272869 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 36185250 |
Mammalian cells contain an evolutionarily conserved type II chaperonin called chaperonin containing tailless complex polypeptide 1 (CCT) or tailless complex polypeptide 1 ring complex (TRiC). The CCT complex is composed of eight subunits [CCT1-8 (yeast) or CCTα-θ (mammals)] and folds substrates needed for cell invasion and proliferation, such as actin, tubulin, and cell division cycle protein 20 homolog (cdc20), as well as oncoproteins like signal transducer and activator of transcription 3 (STAT3), Kirsten rat sarcoma viral oncogene homolog (KRAS), and Myelocytomatosis (MYC). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CDC20 | down-regulates quantity by destabilization
binding
|
FBXO31 |
0.386 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277378 |
|
|
Homo sapiens |
HEK-293T Cell |
| pmid |
sentence |
| 29343641 |
Here we show that the low levels of FBXO31 are maintained through proteasomal degradation by anaphase-promoting complex/cyclosome (APC/C). We find that the APC/C coactivators CDH1 and CDC20 bind to a destruction-box (D-box) motif present in FBXO31 to promote its polyubiquitination and degradation in a cell-cycle-regulated manner, which requires phosphorylation of FBXO31 on serine-33 by the prosurvival kinase AKT. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CDC20 | down-regulates quantity by destabilization
binding
|
SP100 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272724 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 22086178 |
Cdc20 is a co-activator of the anaphase-promoting complex/cyclosome (APC/C complex), which recruits substrates at particular phases of the cell cycle and mediates their degradation. Overexpression of Cdc20 resulted in decreased levels of both endogenous Sp100 protein and overexpressed Sp100 mRNA in HEK 293 cells. Our results suggested that sp100 is a novel substrate of Cdc20 and it is degraded by the ubiquitination pathway. The intact D-box of Sp100 was necessary for this process. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
AURKB | down-regulates activity
phosphorylation
|
CDC20 |
0.768 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-280190 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 28434146 |
When SAC is active, Aurora kinase B (AurkB) phosphorylates and inactivates CDC20, to prevent the activation of anaphase promoting complex and cyclosome (APC/C).|When SAC is active, Aurora kinase B (AurkB) phosphorylates and inactivates CDC20, to prevent the activation of anaphase-promoting complex/cyclosome (APC/C) (Hagting et al., xref ; Nasmyth, xref ; Ruchaud et al., xref ). |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CDK1 | down-regulates activity
phosphorylation
|
CDC20 |
0.935 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279321 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 29971233 |
Cdk1 phosphorylates Cdc20 to negatively regulate its ability to bind and activate the APC/C. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CDC20 | down-regulates quantity by destabilization
binding
|
CCNB1 |
0.965 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272578 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 11285280 |
These results suggested that cyclin A is a target of the Cdc20-associated APC/C in human cells. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CDC20 | down-regulates quantity by destabilization
binding
|
REV1 |
0.278 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-272892 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 23287467 |
Here, we show that human REV1 undergoes proteosomal degradation mediated by the E3 ubiquitin ligase known as anaphase-promoting complex (APC). REV1 associates with APC. Overexpression of APC coactivator CDH1 or CDC20 promotes polyubiquitination and proteosomal degradation of REV1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
CDC20 | form complex 
binding
|
MCC |
0.978 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-265976 |
|
|
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 25092294 |
The mitotic (or spindle assembly) checkpoint system delays anaphase until all chromosomes are correctly attached to the mitotic spindle. When the checkpoint is active, a Mitotic Checkpoint Complex (MCC) assembles and inhibits the ubiquitin ligase Anaphase-Promoting Complex/Cyclosome (APC/C). MCC is composed of the checkpoint proteins Mad2, BubR1, and Bub3 associated with the APC/C activator Cdc20. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
BUB1B | up-regulates activity
phosphorylation
|
CDC20 |
0.993 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279507 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 11030144 |
Furthermore, BUBR1 phosphorylates p55CDC in vitro, and the phosphorylation of p55CDC by BUBR1 appears to be correlated with spindle checkpoint activation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
4.0
CDC20
- 
- 