| + |
NEK2 | up-regulates 
phosphorylation
|
SGO1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-156878 |
Ser14 |
LKKSFQDsLEDIKKR |
Homo sapiens |
|
| pmid |
sentence |
| 17621308 |
Here we show that nek2a phosphorylates human sgo1 and such phosphorylation is essential for faithful chromosome congression in mitosis. phosphorylation sites were mapped to ser(14) and ser(507) |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-156882 |
Ser507 |
TDLCFLNsPIFKQKK |
Homo sapiens |
|
| pmid |
sentence |
| 17621308 |
Here we show that nek2a phosphorylates human sgo1 and such phosphorylation is essential for faithful chromosome congression in mitosis. phosphorylation sites were mapped to ser(14) and ser(507) |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
NEK2 | up-regulates
phosphorylation
|
NDC80 |
0.593 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-94322 |
Ser165 |
LGYPFALsKSSMYTV |
Homo sapiens |
|
| pmid |
sentence |
| 12386167 |
Phosphorylation of the mitotic regulator protein hec1 by nek2 kinase is essential for faithful chromosome segregation.Hec1 (highly expressed in cancer) plays essential roles in chromosome segregation by interacting through its coiled-coil domains with several proteins that modulate the g(2)/m phase.Nek2 phosphorylates hec1 on serine residue 165, both in vitro and in vivo. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NEK2 | up-regulates
phosphorylation
|
NEK2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-151755 |
Ser171 |
RILNHDTsFAKTFVG |
Homo sapiens |
|
| pmid |
sentence |
| 17197699 |
Enzymatic activity, induced; |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-151759 |
Thr170 |
ARILNHDtSFAKTFV |
Homo sapiens |
|
| pmid |
sentence |
| 17197699 |
Thus, it appears that autophosphorylation of thr-170 and/or ser-171 in nek2 may fine-tune overall activity of nek2 in vivo. regardless, the importance of thr-175 suggested by its conservation in many other kinases is underlined by the t175a mutant that shows reduced kinase activity and a significant reduction in efficiency of cs. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-151763 |
Thr175 |
HDTSFAKtFVGTPYY |
Homo sapiens |
|
| pmid |
sentence |
| 17197699 |
Enzymatic activity, induced; |
|
| Publications: |
3 |
Organism: |
Homo Sapiens |
| + |
NEK2 | down-regulates 
phosphorylation
|
CEP250 |
0.776 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-204833 |
Ser2392 |
AGLHHSLsHSLLAVA |
Homo sapiens |
|
| pmid |
sentence |
| 24695856 |
Our data support a model in which centrosome disjunction is triggered by the hyperphosphorylation of c-nap1, a major linker component. This occurs in response to a shift in the balance of activities of the nek2?_Pp1 bi-stable switch. C-nap1 hyperphosphorylation triggers the loss of both oligomerization and, crucially, interaction with the core centriole proximal-end protein, cep135. All three of these sites were identified in our in vivo analysis but only two (s2234 and s2394) were identified as nek2 phosphorylation sites in vitro. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-204837 |
Ser2394 |
LHHSLSHsLLAVAQA |
Homo sapiens |
|
| pmid |
sentence |
| 24695856 |
C-nap1 hyperphosphorylation triggers the loss of both oligomerization and, crucially, interaction with the core centriole proximal-end protein, cep135. All three of these sites were identified in our in vivo analysis but only two (s2234 and s2394) were identified as nek2 phosphorylation sites in vitro. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
NEK2 | down-regulates
phosphorylation
|
NEK2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-151767 |
Ser241 |
RRIPYRYsDELNEII |
Homo sapiens |
|
| pmid |
sentence |
| 17197699 |
Enzymatic activity, inhibited; |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-151771 |
Thr179 |
FAKTFVGtPYYMSPE |
Homo sapiens |
|
| pmid |
sentence |
| 17197699 |
Enzymatic activity, inhibited; |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
NEK2 | down-regulates
phosphorylation
|
TP53 |
0.319 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278488 |
Ser315 |
LPNNTSSsPQPKKKP |
Homo sapiens |
|
| pmid |
sentence |
| 30266789 |
NEK2 Phosphorylates p53 at Ser315 and Reduces Its Stability.|These results are consistent with NEK2 inhibiting p53 transcriptional activation functions. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NEK2 | down-regulates quantity
phosphorylation
|
CTNNB1 |
0.469 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278173 |
Ser33 |
QQQSYLDsGIHSGAT |
Homo sapiens |
|
| pmid |
sentence |
| 30968157 |
NEK2 silencing reduced the phosphorylation of beta-catenin at Ser33 and Ser37, but did not decrease the level of total beta-catenin.|NEK2 slightly decreased the level of total beta-catenin (XREF_FIG). |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278172 |
Ser37 |
YLDSGIHsGATTTAP |
Homo sapiens |
|
| pmid |
sentence |
| 30968157 |
NEK2 silencing reduced the phosphorylation of beta-catenin at Ser33 and Ser37, but did not decrease the level of total beta-catenin.|NEK2 slightly decreased the level of total beta-catenin (XREF_FIG). |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
NEK2 | up-regulates activity
phosphorylation
|
GAS2L1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-273683 |
Ser352 |
HPRSRRYsGDSDSSA |
Homo sapiens |
|
| pmid |
sentence |
| 32289147 |
Nek2A mediates G2/M phosphorylation of GAS2L1. GAS2L1 and its Ser352 phosphorylation are required for proper spindle organization and chromosome segregation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
STK3 | up-regulates
phosphorylation
|
NEK2 |
0.244 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-169539 |
Ser438 |
EKNYQLKsRQILGMR |
Homo sapiens |
|
| pmid |
sentence |
| 21076410 |
Our data suggest that mst2 phosphorylates nek2a thereby recruiting nek2a to centrosomes and promoting phosphorylation and displacement of centrosomal linker proteins |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NEK2 | down-regulates activity
phosphorylation
|
LRRC45 |
0.372 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-273707 |
Ser661 |
VLAYVQAsPVRTLSP |
Homo sapiens |
HeLa Cell |
| pmid |
sentence |
| 24035387 |
Moreover, LRRC45 interacts with both C-Nap1 and rootletin and is phosphorylated by Nek2A at S661 during mitosis. After phosphorylation, both LRRC45 centrosomal localization and fiber-like structures are significantly reduced, which subsequently leads to centrosome separation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NEK2 | up-regulates quantity by stabilization
phosphorylation
|
YAP1 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-276586 |
Thr143 |
AVSPGTLtPTGVVSG |
|
|
| pmid |
sentence |
| 35705994 |
NEK2 promotes the migration and proliferation of ESCC via stabilization of YAP1 by phosphorylation at Thr-143 |
|
| Publications: |
1 |
| + |
NEK2 | up-regulates quantity by stabilization
phosphorylation
|
CD274 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277315 |
Thr194 |
EEKLFNVtSTLRINT |
Mus musculus |
Pancreatic Cancer Cell |
| pmid |
sentence |
| 34315872 |
NEK2 interacts with PD-L1, phosphorylating the T194/T210 residues and preventing ubiquitin-proteasome pathway-mediated degradation of PD-L1 in ER lumen. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-277314 |
Thr210 |
TNEIFYCtFRRLDPE |
Mus musculus |
Pancreatic Cancer Cell |
| pmid |
sentence |
| 34315872 |
NEK2 interacts with PD-L1, phosphorylating the T194/T210 residues and preventing ubiquitin-proteasome pathway-mediated degradation of PD-L1 in ER lumen. |
|
| Publications: |
2 |
Organism: |
Mus Musculus |
| + |
NEK2 | down-regulates
phosphorylation
|
PPP1CC |
0.488 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-78306 |
Thr307 |
EKKKPNAtRPVTPPR |
Homo sapiens |
|
| pmid |
sentence |
| 10880350 |
Pp1 is a substrate for nek2 and phosphorylation of pp1gamma(1) on two c-terminal sites reduces its phosphatase activity. / threonine-307 and -318 appear to be equally well phosphorylated by nek2 |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-78603 |
Thr318 |
TPPRGMItKQAKK |
Homo sapiens |
|
| pmid |
sentence |
| 10880350 |
Pp1 is a substrate for nek2 and phosphorylation of pp1gamma(1) on two c-terminal sites reduces its phosphatase activity. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
NEK2 | up-regulates activity
phosphorylation
|
KIF24 |
0.697 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278413 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 26290419 |
Nek2 binds and phosphorylates Kif24.|We also provide evidence that Nek2 dependent phosphorylation induces a conformational change in Kif24 that promotes its activity. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NEK2 | down-regulates quantity by destabilization
phosphorylation
|
CEP68 |
0.252 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279471 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 25704143 |
In this study we show phosphorylation of Cep68 by Nek2 creates a potential phosphodegron that directs Cep68 destruction through the activity of SCF-beta-Trcp.|Our above results showed that Nek2 promoted mitotic degradation of Cep68. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NEK2 | down-regulates activity
phosphorylation
|
CNTROB |
0.351 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279545 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 23291182 |
The opposite outcomes in NEK2- and centrobin-depleted cells suggest that NEK2 antagonizes biological functions of centrobin.|These results suggest that NEK2 phosphorylates specific sites of centrobin, which are distinct from the PLK1 phosphorylation sites. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PCM1 | up-regulates
relocalization
|
NEK2 |
0.427 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-133337 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 15659651 |
Recruitment of nek2 and c-nap1 to the centrosome is dependent on pcm-1 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NEK2 | up-regulates activity
phosphorylation
|
SUFU |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279235 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 27297360 |
Intriguingly, Nek2A is found to stabilize SuFu at least partly depending on its kinase activity, thereby triggering phosphorylation of the SuFu protein.|Nek2A phosphorylates and stabilizes SuFu: A new strategy of Gli2/Hedgehog signaling regulatory mechanism. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NEK2 | down-regulates activity
phosphorylation
|
SRSF1 |
0.386 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279344 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 24369428 |
First, NEK2 interacts with and phosphorylates SRSF1. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NEK2 | down-regulates activity
phosphorylation
|
MAD2L1 |
0.844 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278446 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20034488 |
We demonstrated that overexpression of Nek2 can enhance the ability of Mad2 to cause delays in cell division.|We have demonstrated that Nek2 can bind and phosphorylate Mad2 and Cdc20. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NEK2 | down-regulates activity
phosphorylation
|
CCDC102B |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-275626 |
|
|
|
|
| pmid |
sentence |
| 30404835 |
CCDC102B is recruited to the centrosome by C-Nap1 (also known as CEP250) and interacts with the centrosome linker components rootletin and LRRC45. CCDC102B decorates and facilitates the formation of rootletin filaments. Furthermore, CCDC102B is phosphorylated by Nek2A (an isoform encoded by NEK2) and is disassociated from the centrosome at the onset of mitosis. |
|
| Publications: |
1 |
| + |
NEK2 | down-regulates
phosphorylation
|
PP1 |
0.489 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264655 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 10880350 |
Pp1 is a substrate for nek2 and phosphorylation of pp1gamma(1) on two c-terminal sites reduces its phosphatase activity. / threonine-307 and -318 appear to be equally well phosphorylated by nek2 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PPP1CA | down-regulates
dephosphorylation
|
NEK2 |
0.373 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-152949 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17283141 |
Nek2 is activated by autophosphorylation, and its dephosphorylation is catalyzed by pp1 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NEK2 | down-regulates activity
phosphorylation
|
NCAPD2 |
0.27 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279234 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 25704143 |
Nek2 phosphorylates C-Nap1, rootletin and beta-catenin to regulate centrosome separation. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
PP1 | down-regulates
dephosphorylation
|
NEK2 |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-264667 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 17283141 |
Nek2 is activated by autophosphorylation, and its dephosphorylation is catalyzed by pp1 |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NEK2 | up-regulates activity
phosphorylation
|
CDC20 |
0.945 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278366 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 20034488 |
In summary, we have demonstrated that Nek2 can associate with and phosphorylate Mad2 and Cdc20.|The results presented here support a model in which Nek2 modulates the functions of Mad2 and Cdc20 in the mitotic checkpoint and elevation of Nek2 levels may contribute to chromosome instability by interfering with the control of the checkpoint. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
NEK2 | up-regulates
phosphorylation
|
NEK11 |
0.392 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-124944 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 15161910 |
Nek2 directly phosphorylated nek11 in the c-terminal non-catalytic region and elevated nek11 kinase activity. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
4.0
NEK2
- 
- 