| + |
LNX1 | down-regulates quantity 
ubiquitination
|
SUFU |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278626 |
Lys470 |
SWPEKKLkVSILPDV |
Homo sapiens |
|
| pmid |
sentence |
| 33608498 |
Indeed, they target different lysine residues in the SuFu protein, as LNX1 ubiquitinates SuFu at K59 and K470, and SCF Fbxl17 acts at K257, while Itch ubiquitinates at K321 and K457.|XREF_FIG, ectopic LNX1 expression reduced SuFu protein levels in HEK-293T cells, while shRNA mediated knockdown of LNX1 increased these levels. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-278627 |
Lys59 |
LQVTAIVkYWLGGPD |
Homo sapiens |
|
| pmid |
sentence |
| 33608498 |
Indeed, they target different lysine residues in the SuFu protein, as LNX1 ubiquitinates SuFu at K59 and K470, and SCF Fbxl17 acts at K257, while Itch ubiquitinates at K321 and K457.|XREF_FIG, ectopic LNX1 expression reduced SuFu protein levels in HEK-293T cells, while shRNA mediated knockdown of LNX1 increased these levels. |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| + |
PRKACA | up-regulates 
phosphorylation
|
SUFU |
0.469 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-200492 |
Ser342 |
LAHDRAPsRKDSLES |
Homo sapiens |
|
| pmid |
sentence |
| 23337587 |
Interestingly, sufu stability is regulated via dual phosphorylation at ser342/ser346 by pka and gsk3, and blocking sufu phosphorylation either by mutating ser346 to ala or by treating cultured cells with pka inhibitors attenuates sufu ciliary accumulation, whereas phospho-mimetic forms of sufu exhibits increased ciliary localization |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-119099 |
Ser346 |
RAPSRKDsLESDSST |
Homo sapiens |
|
| pmid |
sentence |
| 23337587 |
Interestingly, sufu stability is regulated via dual phosphorylation at ser342/ser346 by pka and gsk3, and blocking sufu phosphorylation either by mutating ser346 to ala or by treating cultured cells with pka inhibitors attenuates sufu ciliary accumulation, whereas phospho-mimetic forms of sufu exhibits increased ciliary localization |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-200496 |
Ser346 |
RAPSRKDsLESDSST |
Homo sapiens |
|
| pmid |
sentence |
| 21317289 |
We report that Sufu is phosphorylated at Ser-342 and Ser-346 by GSK3? and cAMP-dependent protein kinase A (PKA), respectively, and phosphorylation at this dual site stabilizes Sufu against Shh signaling-induced degradation |
|
| Publications: |
3 |
Organism: |
Homo Sapiens |
| Pathways: | Sonic Hedgehog |
| + |
PRKACA | up-regulates quantity by stabilization
phosphorylation
|
SUFU |
0.469 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-171999 |
Ser342 |
LAHDRAPsRKDSLES |
Homo sapiens |
|
| pmid |
sentence |
| 21317289 |
We report that Sufu is phosphorylated at Ser-342 and Ser-346 by GSK3? and cAMP-dependent protein kinase A (PKA), respectively, and phosphorylation at this dual site stabilizes Sufu against Shh signaling-induced degradation. |
|
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-172003 |
Ser346 |
RAPSRKDsLESDSST |
Homo sapiens |
|
| pmid |
sentence |
| 21317289 |
We report that Sufu is phosphorylated at Ser-342 and Ser-346 by GSK3? and cAMP-dependent protein kinase A (PKA), respectively, and phosphorylation at this dual site stabilizes Sufu against Shh signaling-induced degradation |
|
| Publications: |
2 |
Organism: |
Homo Sapiens |
| Pathways: | Sonic Hedgehog |
| + |
NEK2 | up-regulates activity
phosphorylation
|
SUFU |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-279235 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 27297360 |
Intriguingly, Nek2A is found to stabilize SuFu at least partly depending on its kinase activity, thereby triggering phosphorylation of the SuFu protein.|Nek2A phosphorylates and stabilizes SuFu: A new strategy of Gli2/Hedgehog signaling regulatory mechanism. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SUFU | down-regulates activity
relocalization
|
GLI2 |
0.91 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129065 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 16316410 |
We demonstrate here that Su(fu) prevents the nuclear accumulation of Gli1 and Gli2 through multiple mechanisms |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| Pathways: | Sonic Hedgehog |
| + |
SUFU | down-regulates
binding
|
GLIS3 |
0.379 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-173573 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 21543335 |
These data indicate that the inhibition of glis3-mediated transactivation by sufu appears to rely on the interaction with glis3 through the ygh motif and is not related to an effect on the general transcriptional machinery |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SUFU | up-regulates
binding
|
SAP18 |
0.675 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-117311 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 11960000 |
Here we report that the mouse homolog of su(fu) [msu(fu)] specifically interacts with sap18, a component of the msin3 and histone deacetylase complex. In addition, we demonstrate that msu(fu) functionally cooperates with sap18 to repress transcription by recruiting the sap18-msin3 complex to promoters containing the gli-binding element. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SUFU | down-regulates activity
binding
|
GLI1 |
0.949 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-249591 |
|
|
|
|
| pmid |
sentence |
| 15367681 |
Here we characterize structural and functional determinants of Su(fu) required for Gli regulation and show that Su(fu) contains at least two distinct domains: a highly conserved carboxy-terminal region required for binding to the amino-terminal ends of the Gli proteins and a unique amino-terminal domain that binds the carboxy-terminal tail of Gli1. While each domain is capable of binding to different Gli1 regions independently, interactions between Su(fu) and Gli1 at both sites are required for cytoplasmic tethering and repression of Gli1 |
|
| Publications: |
1 |
| Pathways: | Sonic Hedgehog |
| + |
FBXW11 | up-regulates
binding
|
SUFU |
0.292 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-72240 |
|
|
Homo sapiens |
Prostate Gland Cancer Cell, Melanoma Cell, Glioblastoma Cell |
| pmid |
sentence |
| 10564661 |
We found that in vitro-translated 35s- labeled slimb indeed specifically bound to su(fu) in the gst pull-down assay. In our functional gli reporter assay, slimb alone did not alter gli-induced reporter expression;however, when cotransfected with hsu(fu), slimb significantly potentiated the inhibitory effect of su(fu) on gli activity. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
SUFU | up-regulates quantity by stabilization
binding
|
GLI2 |
0.91 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-268867 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 20463034 |
We show that loss of suppressor of fused (Sufu; an inhibitory effector for Gli proteins) results in destabilization of Gli2 and Gli3 full-length activators but not of their C-terminally processed repressors, whereas overexpression of Sufu stabilizes them. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Sonic Hedgehog |
| + |
SUFU | down-regulates
relocalization
|
GLIS2 |
0.265 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-142608 |
|
|
Homo sapiens |
Prostate Gland Cancer Cell |
| pmid |
sentence |
| 16316410 |
Negative regulation of gli1 and gli2 activator function by suppressor of fused through multiple mechanisms.Together, these observations reveal that su(fu) regulates the activity of gli1 and gli2 through distinct cytoplasmic and nuclear mechanisms. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| + |
FBXL17 | down-regulates quantity by destabilization
ubiquitination
|
SUFU |
0.419 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-253545 |
|
|
|
|
| pmid |
sentence |
| 27234298 |
Here, we show that Fbxl17 (F-box and leucine-rich repeat protein 17) targets Sufu for proteolysis in the nucleus. The ubiquitylation of Sufu, mediated by Fbxl17, allows the release of Gli1 from Sufu for proper Hh signal transduction |
|
| Publications: |
1 |
| + |
SUFU | up-regulates
binding
|
SUFU |
0.2 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-72311 |
|
|
Homo sapiens |
Prostate Gland Cancer Cell, Melanoma Cell, Glioblastoma Cell |
| pmid |
sentence |
| 10564661 |
Hsu(fu) associated with itself / homo- or heterodimers of hsu(fu) might function to bring together other effector proteins |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Sonic Hedgehog |
| + |
SUFU | down-regulates
relocalization
|
GLI3 |
0.889 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-72308 |
|
|
Homo sapiens |
Prostate Gland Cancer Cell, Melanoma Cell, Glioblastoma Cell |
| pmid |
sentence |
| 10564661 |
Su(fu) is a negative regulator of shh that interacts with all three gli proteins to retain them in the cytosol. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Sonic Hedgehog |
| + |
SMO | down-regulates activity
binding
|
SUFU |
0.644 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-177656 |
|
|
Homo sapiens |
Fibroblast |
| pmid |
sentence |
| 22114142 |
In addition to activating g(i), smo signals through its c-terminal tail to inhibit suppressor of fused, resulting in stabilization and activation of the gli family of transcription factors, which execute a transcriptional response to so-called canonical hh signaling. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Sonic Hedgehog |
| + |
SUFU | up-regulates quantity by stabilization
binding
|
GLI3 |
0.889 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-268868 |
|
|
Homo sapiens |
HEK-293 Cell |
| pmid |
sentence |
| 20463034 |
We show that loss of suppressor of fused (Sufu; an inhibitory effector for Gli proteins) results in destabilization of Gli2 and Gli3 full-length activators but not of their C-terminally processed repressors, whereas overexpression of Sufu stabilizes them. |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Pathways: | Sonic Hedgehog |
| + |
MTSS1 | up-regulates
binding
|
SUFU |
0.44 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-130545 |
|
|
Homo sapiens |
|
| pmid |
sentence |
| 15545630 |
We found that in vitro translated gli1 and sufu bind to gst-mim, but not gst-mim?N399 Or gst columns (fig. 4f). Indicative of the importance of the mim/gli complex interactions, the mim?N399 Mutant that fails to interact with gli and sufu showed a markedly reduced capacity to potentiate gli-dependent transcription (fig. 4g). Although these results indicate that a mim/gli/sufu complex is important for mim-mediated transcriptional potentiation |
|
| Publications: |
1 |
Organism: |
Homo Sapiens |
| Tissue: |
Skin |
| + |
SUFU | down-regulates activity
relocalization
|
GLI3 |
0.889 |
| Identifier |
Residue |
Sequence |
Organism |
Cell Line |
| SIGNOR-129068 |
|
|
Mus musculus |
|
| pmid |
sentence |
| 10433919 |
Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling |
|
| Publications: |
1 |
Organism: |
Mus Musculus |
| Pathways: | Sonic Hedgehog |
4.0
SUFU
- 
- 