+ |
TBK1 | down-regulates quantity by destabilization
phosphorylation
|
TNIP1 |
0.383 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-275733 |
Ser122 |
KPPSSGTsSEFEVVT |
|
|
pmid |
sentence |
36574265 |
TBK1 phosphorylation activates LIR-dependent degradation of the inflammation repressor TNIP1 |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-275734 |
Ser123 |
PPSSGTSsEFEVVTP |
|
|
pmid |
sentence |
36574265 |
TBK1 phosphorylation activates LIR-dependent degradation of the inflammation repressor TNIP1 |
|
Publications: |
2 |
+ |
TBK1 | up-regulates activity
phosphorylation
|
HTT |
0.294 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-270350 |
Ser13 |
KLMKAFEsLKSFQQQ |
Homo sapiens |
HEK-293 Cell |
pmid |
sentence |
32757223 |
Herein, we report the discovery and validation of a kinase, TANK-binding kinase 1 (TBK1), that efficiently phosphorylates full-length and N-terminal HTT fragments in vitro (at S13/S16), in cells (at S13) and in vivo. TBK1 expression in HD models (cells, primary neurons, and Caenorhabditis elegans) increases mutant HTT exon 1 phosphorylation and reduces its aggregation and cytotoxicity. |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
+ |
TBK1 | up-regulates quantity
phosphorylation
|
SIKE1 |
0.707 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-273815 |
Ser133 |
PVLKAHQsHSAEIES |
Homo sapiens |
HEK-293 Cell |
pmid |
sentence |
23649622 |
TBK1 phosphorylates SIKE on six serine residues that mimic the IRF3 phosphorylation sequence.Serines are listed from left to right: 133, 185, 187, 188, 190, and 198. |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-273813 |
Ser185 |
KELRELLsISSESLQ |
Homo sapiens |
HEK-293 Cell |
pmid |
sentence |
23649622 |
TBK1 phosphorylates SIKE on six serine residues that mimic the IRF3 phosphorylation sequence.TBK1-SIKE interactions were modulated by SIKE phosphorylation, clustered in the C-terminal portion of SIKE (Ser-133, -185, -187, -188, -190, and -198). Here, we describe the mechanism by which suppressor of IKKε (SIKE) inhibits TBK1-mediated phosphorylation of interferon regulatory factor 3 (IRF3), which is essential to type I interferon production. Kinetic analyses showed that SIKE not only inhibits IRF3 phosphorylation but is also a high affinity TBK1 substrate. |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-273814 |
Ser187 |
LRELLSIsSESLQAR |
Homo sapiens |
HEK-293 Cell |
pmid |
sentence |
23649622 |
TBK1 phosphorylates SIKE on six serine residues that mimic the IRF3 phosphorylation sequence.Serines are listed from left to right: 133, 185, 187, 188, 190, and 198. |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-273816 |
Ser188 |
RELLSISsESLQARK |
Homo sapiens |
HEK-293 Cell |
pmid |
sentence |
23649622 |
TBK1 phosphorylates SIKE on six serine residues that mimic the IRF3 phosphorylation sequence.Serines are listed from left to right: 133, 185, 187, 188, 190, and 198. |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-273817 |
Ser190 |
LLSISSEsLQARKEN |
Homo sapiens |
HEK-293 Cell |
pmid |
sentence |
23649622 |
TBK1 phosphorylates SIKE on six serine residues that mimic the IRF3 phosphorylation sequence.Serines are listed from left to right: 133, 185, 187, 188, 190, and 198. |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-273818 |
Ser198 |
LQARKENsMDTASQA |
Homo sapiens |
HEK-293 Cell |
pmid |
sentence |
23649622 |
TBK1 phosphorylates SIKE on six serine residues that mimic the IRF3 phosphorylation sequence.Serines are listed from left to right: 133, 185, 187, 188, 190, and 198. |
|
Publications: |
6 |
Organism: |
Homo Sapiens |
+ |
TBK1 | down-regulates quantity by destabilization
phosphorylation
|
PBXIP1 |
0.294 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-273811 |
Ser147 |
REEGRCSsSDDDTDV |
Homo sapiens |
HEK-293 Cell |
pmid |
sentence |
24488098 |
MDM2-dependent HPIP degradation occurs in breast cancer cells on its phosphorylation by the estrogen-activated kinase TBK1. Phosphorylation of HPIP on serine 147 by TBK1 promotes E2-mediated GREB1 expression. |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-273643 |
Ser147 |
REEGRCSsSDDDTDV |
Homo sapiens |
HEK-293 Cell |
pmid |
sentence |
24488098 |
Phosphorylation of HPIP on serine 147 by TBK1 promotes E2-mediated GREB1 expression. Accordingly, we identified the microtubule-associated HPIP, a positive regulator of oncogenic AKT signaling, as a novel MDM2 substrate. MDM2-dependent HPIP degradation occurs in breast cancer cells on its phosphorylation by the estrogen-activated kinase TBK1. |
|
Publications: |
2 |
Organism: |
Homo Sapiens |
+ |
TBK1 | up-regulates
phosphorylation
|
IRF5 |
0.557 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-196528 |
Ser158 |
QRMLPSLsLTEDVKW |
Homo sapiens |
|
pmid |
sentence |
22412986 |
Activation of interferon regulatory factor 5 by site specific phosphorylation. Although the gene induction by irf5 in the presence of tbk-1 was modest, phosphorylation by tbk-1 produced a significant shift in the mobility of irf5 in sds-page. For this reason we identified the residues that are phosphorylated on irf5 by tbk-1 with mass spectrometry. Ser-158 and ser-309 were found to be phosphorylated |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-196532 |
Ser293 |
VELFGPIsLEQVRFP |
Homo sapiens |
|
pmid |
sentence |
22412986 |
Activation of interferon regulatory factor 5 by site specific phosphorylation. Although the gene induction by irf5 in the presence of tbk-1 was modest, phosphorylation by tbk-1 produced a significant shift in the mobility of irf5 in sds-page. For this reason we identified the residues that are phosphorylated on irf5 by tbk-1 with mass spectrometry. Ser-158 and ser-309 were found to be phosphorylated |
|
Publications: |
2 |
Organism: |
Homo Sapiens |
+ |
PPM1A | down-regulates activity
dephosphorylation
|
TBK1 |
0.42 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-276966 |
Ser172 |
EDDEQFVsLYGTEEY |
Homo sapiens |
|
pmid |
sentence |
27419230 |
Furthermore, PPM1A, but not PPM1B, serves as an efficient phosphatase to dephosphorylate Ser 172 residue of both TBK1 and IKKepsilon kinases, which is critical for their kinase activities.|In a similar in vitro phosphatase assay, incubation of PPM1A also eliminated TBK1 and IKKepsilon phosphorylation at Ser 172 residue, evidenced by phospho-S172 immunoblotting (XREF_FIG, F and G).|These observations suggest that PPM1A may block kinase activities of TBK1 and IKKepsilon. |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
+ |
TBK1 | up-regulates activity
phosphorylation
|
STX17 |
0.296 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-273812 |
Ser202 |
SQQEKIDsIADHVNS |
Homo sapiens |
HEK-293 Cell |
pmid |
sentence |
30827897 |
Stx17 is phosphorylated by TBK1 whereby phospho-Stx17 controls the formation of the ATG13+FIP200+ mammalian pre-autophagosomal structure (mPAS) in response to induction of autophagy. TBK1 phosphorylates Stx17 at S202. |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
+ |
TBK1 | down-regulates activity
phosphorylation
|
DDAH2 |
0.2 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-275648 |
Ser245 |
GGGDLPNsQEALQKL |
|
|
pmid |
sentence |
33850055 |
TANK-binding kinase 1 (TBK1), a kinase downstream of MAVS, inhibited DDAH2 by phosphorylating DDAH2 at multiple sites. |The T203D, T211D, S245D, and S253D mutations significantly reduced the inhibitory effect of DDAH2 on RLR signaling, suggesting that phosphorylation of these residues was critical for DDAH2 to inhibit activation o |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-275646 |
Ser253 |
QEALQKLsDVTLVPV |
|
|
pmid |
sentence |
33850055 |
TANK-binding kinase 1 (TBK1), a kinase downstream of MAVS, inhibited DDAH2 by phosphorylating DDAH2 at multiple sites. |The T203D, T211D, S245D, and S253D mutations significantly reduced the inhibitory effect of DDAH2 on RLR signaling, suggesting that phosphorylation of these residues was critical for DDAH2 to inhibit activation o |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-275645 |
Thr203 |
VRAMAVLtDHPYASL |
|
|
pmid |
sentence |
33850055 |
TANK-binding kinase 1 (TBK1), a kinase downstream of MAVS, inhibited DDAH2 by phosphorylating DDAH2 at multiple sites. |The T203D, T211D, S245D, and S253D mutations significantly reduced the inhibitory effect of DDAH2 on RLR signaling, suggesting that phosphorylation of these residues was critical for DDAH2 to inhibit activation o |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-275647 |
Thr211 |
DHPYASLtLPDDAAA |
|
|
pmid |
sentence |
33850055 |
TANK-binding kinase 1 (TBK1), a kinase downstream of MAVS, inhibited DDAH2 by phosphorylating DDAH2 at multiple sites. |The T203D, T211D, S245D, and S253D mutations significantly reduced the inhibitory effect of DDAH2 on RLR signaling, suggesting that phosphorylation of these residues was critical for DDAH2 to inhibit activation o |
|
Publications: |
4 |
+ |
TBK1 | up-regulates activity
phosphorylation
|
STING1 |
0.2 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-263136 |
Ser358 |
VPSTSTMsQEPELLI |
Homo sapiens |
HEK-293 Cell |
pmid |
sentence |
18818105 |
MITA was phosphorylated by TBK1, which is required for MITA-mediated activation of IRF3. Our results suggest that MITA is a critical mediator of virus-triggered IRF3 activation and IFN expression and further demonstrate the importance of certain mitochondrial proteins in innate antiviral immunity. Consistent with its inability to activate IRF-E, the mutation of S358 to alanine impaired the ability of MITA to interact with TBK1 and to enhance the interaction between TBK1 and IRF3 |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
Pathways: | COVID-19 Causal Network, Innate Immune Response, SARS-CoV INNATE RESPONSE TO dsRNA |
+ |
TBK1 | down-regulates quantity by destabilization
phosphorylation
|
NFKBIA |
0.468 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-246643 |
Ser36 |
RHDSGLDsMKDEEYE |
Homo sapiens |
|
pmid |
sentence |
11815618 |
Nuclear factor-kappaB activation depends on phosphorylation and degradation of its inhibitor protein, IkapapB. TBK-1 and IKK-i phosphorylate Ser36 of IkappaBalpha. |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
Pathways: | COVID-19 Causal Network, EBV infection, Toll like receptors |
+ |
TBK1 | up-regulates activity
phosphorylation
|
IRF3 |
0.816 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-178391 |
Ser385 |
MARVGGAsSLENTVD |
in vitro |
|
pmid |
sentence |
18440553 |
Virus-induced phosphoactivation of irf-3, thought to be mediated directly or indirectly by ikk? And/or tbk1 occurs in the c-terminal region of irf-3 at seven ser/thr residues, 385sslentvdlhisnshplslts405 (fig. 1a).Within This region, irf-3 has two phosphorylation sites: site 1 includes ser385 and ser386, whereas site 2 includes ser396, ser398, ser402, ser405, and thr404. |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-178395 |
Ser386 |
ARVGGASsLENTVDL |
in vitro |
|
pmid |
sentence |
18440553 |
Virus-induced phosphoactivation of irf-3, thought to be mediated directly or indirectly by ikk? And/or tbk1 occurs in the c-terminal region of irf-3 at seven ser/thr residues, 385sslentvdlhisnshplslts405 (fig. 1a).Within This region, irf-3 has two phosphorylation sites: site 1 includes ser385 and ser386, whereas site 2 includes ser396, ser398, ser402, ser405, and thr404. |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-178399 |
Ser396 |
NTVDLHIsNSHPLSL |
in vitro |
|
pmid |
sentence |
18440553 |
Virus-induced phosphoactivation of irf-3, thought to be mediated directly or indirectly by ikk? And/or tbk1 occurs in the c-terminal region of irf-3 at seven ser/thr residues, 385sslentvdlhisnshplslts405 (fig. 1a).Within This region, irf-3 has two phosphorylation sites: site 1 includes ser385 and ser386, whereas site 2 includes ser396, ser398, ser402, ser405, and thr404. |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-178403 |
Ser398 |
VDLHISNsHPLSLTS |
in vitro |
|
pmid |
sentence |
18440553 |
Virus-induced phosphoactivation of irf-3, thought to be mediated directly or indirectly by ikk? And/or tbk1 occurs in the c-terminal region of irf-3 at seven ser/thr residues, 385sslentvdlhisnshplslts405 (fig. 1a).Within This region, irf-3 has two phosphorylation sites: site 1 includes ser385 and ser386, whereas site 2 includes ser396, ser398, ser402, ser405, and thr404. |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-178407 |
Ser402 |
ISNSHPLsLTSDQYK |
in vitro |
|
pmid |
sentence |
18440553 |
Virus-induced phosphoactivation of irf-3, thought to be mediated directly or indirectly by ikk? And/or tbk1 occurs in the c-terminal region of irf-3 at seven ser/thr residues, 385sslentvdlhisnshplslts405 (fig. 1a).Within This region, irf-3 has two phosphorylation sites: site 1 includes ser385 and ser386, whereas site 2 includes ser396, ser398, ser402, ser405, and thr404. |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-178411 |
Ser405 |
SHPLSLTsDQYKAYL |
in vitro |
|
pmid |
sentence |
18440553 |
Virus-induced phosphoactivation of irf-3, thought to be mediated directly or indirectly by ikk? And/or tbk1 occurs in the c-terminal region of irf-3 at seven ser/thr residues, 385sslentvdlhisnshplslts405 (fig. 1a).Within This region, irf-3 has two phosphorylation sites: site 1 includes ser385 and ser386, whereas site 2 includes ser396, ser398, ser402, ser405, and thr404. |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-178420 |
Thr404 |
NSHPLSLtSDQYKAY |
in vitro |
|
pmid |
sentence |
18440553 |
Virus-induced phosphoactivation of irf-3, thought to be mediated directly or indirectly by ikk? And/or tbk1 occurs in the c-terminal region of irf-3 at seven ser/thr residues, 385sslentvdlhisnshplslts405 (fig. 1a).Within This region, irf-3 has two phosphorylation sites: site 1 includes ser385 and ser386, whereas site 2 includes ser396, ser398, ser402, ser405, and thr404. |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-260154 |
|
|
Homo sapiens |
|
pmid |
sentence |
24622840 |
STING recruits TBK1 and IKKε and forms the TBK1-IKKε complex via the association with TRAF3. The TBK1 complex induces the phosphorylation, dimerization, and nuclear translocation of IRF3. |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-120355 |
|
|
Homo sapiens |
T-lymphocyte |
pmid |
sentence |
14679297 |
We show that purified recombinant ikk-epsilon and tbk1 directly phosphorylate the critical serine residues in irf3 allowing its translocation into the nucleus and production of interferon type i. |
|
Publications: |
9 |
Organism: |
In Vitro, Homo Sapiens |
Pathways: | COVID-19 Causal Network, EBV infection, Innate Immune Response, Inflammosome Activation, SARS-CoV INFLAMMATORY RESPONSE, SARS-CoV INNATE RESPONSE TO dsRNA, Toll like receptors |
+ |
TBK1 | up-regulates
phosphorylation
|
SQSTM1 |
0.701 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-191944 |
Ser403 |
ESLSQMLsMGFSDEG |
Homo sapiens |
Macrophage |
pmid |
sentence |
22921120 |
Tbk-1 coordinated assembly and function of the autophagic machinery and phosphorylated the autophagic adaptor p62 (sequestosome 1) on ser-403, a residue essential for its role in autophagic clearance. |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
+ |
TBK1 | up-regulates
phosphorylation
|
STAT6 |
0.66 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-176771 |
Ser407 |
PIQLQALsLPLVVIV |
Homo sapiens |
|
pmid |
sentence |
22000020 |
We now show that stat6 is required for innate immune signaling in response to virus infection. Viruses or cytoplasmic nucleic acids trigger sting (also named mita/eris) to recruit stat6 to the endoplasmic reticulum, leading to stat6 phosphorylation on ser(407) by tbk1 and tyr(641), independent of jaks. Phosphorylated stat6 then dimerizes and translocates to the nucleus to induce specific target genes responsible for immune cell homing. |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-176775 |
Tyr641 |
MGKDGRGyVPATIKM |
Homo sapiens |
|
pmid |
sentence |
22000020 |
We now show that stat6 is required for innate immune signaling in response to virus infection. Viruses or cytoplasmic nucleic acids trigger sting (also named mita/eris) to recruit stat6 to the endoplasmic reticulum, leading to stat6 phosphorylation on ser(407) by tbk1 and tyr(641), independent of jaks. Phosphorylated stat6 then dimerizes and translocates to the nucleus to induce specific target genes responsible for immune cell homing. |
|
Publications: |
2 |
Organism: |
Homo Sapiens |
+ |
TBK1 | up-regulates
phosphorylation
|
AKT1 |
0.417 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-252608 |
Ser473 |
RPHFPQFsYSASGTA |
Homo sapiens |
|
pmid |
sentence |
21329883 |
Upon mitogen stimulation, triggering of the innate immune response, re-exposure to glucose, or oncogene activation, tbk1 is recruited to the exocyst, where it activates akt. Akt is a direct tbk1 substrate that connects tbk1 to prosurvival signaling. |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
+ |
TBK1 | up-regulates
phosphorylation
|
AKT |
0.417 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-172132 |
Ser473 |
RPHFPQFsYSASGTA |
Homo sapiens |
|
pmid |
sentence |
21329883 |
Upon mitogen stimulation, triggering of the innate immune response, re-exposure to glucose, or oncogene activation, tbk1 is recruited to the exocyst, where it activates akt. Akt is a direct tbk1 substrate that connects tbk1 to prosurvival signaling. |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
Pathways: | COVID-19 Causal Network, EBV infection, Inflammosome Activation, SARS-CoV INFLAMMATORY RESPONSE |
+ |
DYRK2 | down-regulates quantity by destabilization
phosphorylation
|
TBK1 |
0.422 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-276939 |
Ser527 |
QDIDSRLsPGGSLAD |
Homo sapiens |
HEK-293 Cell |
pmid |
sentence |
26407194 |
We further found that DYRK2 phosphorylated Ser527 of TBK1, which is essential for the recruitment of NLRP4 and for the E3 ubiquitin ligase DTX4 to degrade TBK1. |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
+ |
TBK1 | up-regulates
phosphorylation
|
RELA |
0.602 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-129951 |
Ser536 |
SGDEDFSsIADMDFS |
Homo sapiens |
|
pmid |
sentence |
15489227 |
Chromatographic fractionation of cell extracts allowed the identification of two distinct enzymatic activities phosphorylating ser-536. Peak 1 represents an unknown kinase, whereas peak 2 contained ikkalpha, ikkbeta, ikkepsilon, and tbk1. collectively, our results provide evidence for at least five kinases that converge on ser-536 of p65 and a novel function for this phosphorylation site in the recruitment of components of the basal transcriptional machinery to the interleukin-8 promoter. |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
+ |
PRKCQ | up-regulates activity
phosphorylation
|
TBK1 |
0.2 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-272472 |
Ser716 |
HILERFGsLTMDGGL |
Homo sapiens |
|
pmid |
sentence |
31792381 |
TBKBP1 recruits TBK1 to protein kinase C-theta (PKCθ) through a scaffold protein, CARD10. This enables PKCθ to phosphorylate TBK1 at Ser 716, a crucial step for TBK1 activation |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
+ |
TBK1 | up-regulates activity
phosphorylation
|
PRPS1 |
0.2 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-277316 |
Thr228 |
DMADTCGtICHAADK |
in vitro |
|
pmid |
sentence |
34343500 |
Here, we show that ionizing radiation results in TBK1-mediated phosphorylation of phosphoribosyl pyrophosphate synthetase (PRPS)1/2 at T228, thereby enhancing PRPS1/2 catalytic activity and promoting deoxyribonucleotide synthesis. |
|
Publications: |
1 |
Organism: |
In Vitro |
+ |
TBK1 | up-regulates activity
phosphorylation
|
PRPS2 |
0.2 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-277317 |
Thr228 |
DMADTCGtICHAADK |
in vitro |
|
pmid |
sentence |
34343500 |
Here, we show that ionizing radiation results in TBK1-mediated phosphorylation of phosphoribosyl pyrophosphate synthetase (PRPS)1/2 at T228, thereby enhancing PRPS1/2 catalytic activity and promoting deoxyribonucleotide synthesis. |
|
Publications: |
1 |
Organism: |
In Vitro |
+ |
HCK | down-regulates activity
phosphorylation
|
TBK1 |
0.2 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-276726 |
Tyr354 |
SSNQELIyEGRRLVL |
Homo sapiens |
HEK-293T Cell |
pmid |
sentence |
28618271 |
The Src family kinases (SFKs) Lck, Hck, and Fgr directly phosphorylate TBK1 at Tyr354/394, to prevent TBK1 dimerization and activation. |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-276727 |
Tyr394 |
LNTIGLIyEKISLPK |
Homo sapiens |
HEK-293T Cell |
pmid |
sentence |
28618271 |
The Src family kinases (SFKs) Lck, Hck, and Fgr directly phosphorylate TBK1 at Tyr354/394, to prevent TBK1 dimerization and activation. |
|
Publications: |
2 |
Organism: |
Homo Sapiens |
+ |
LCK | down-regulates activity
phosphorylation
|
TBK1 |
0.2 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-276724 |
Tyr354 |
SSNQELIyEGRRLVL |
Homo sapiens |
HEK-293T Cell |
pmid |
sentence |
28618271 |
The Src family kinases (SFKs) Lck, Hck, and Fgr directly phosphorylate TBK1 at Tyr354/394, to prevent TBK1 dimerization and activation. |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-276723 |
Tyr394 |
LNTIGLIyEKISLPK |
Homo sapiens |
HEK-293T Cell |
pmid |
sentence |
28618271 |
The Src family kinases (SFKs) Lck, Hck, and Fgr directly phosphorylate TBK1 at Tyr354/394, to prevent TBK1 dimerization and activation. |
|
Publications: |
2 |
Organism: |
Homo Sapiens |
+ |
FGR | down-regulates activity
phosphorylation
|
TBK1 |
0.2 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-276725 |
Tyr354 |
SSNQELIyEGRRLVL |
Homo sapiens |
HEK-293T Cell |
pmid |
sentence |
28618271 |
The Src family kinases (SFKs) Lck, Hck, and Fgr directly phosphorylate TBK1 at Tyr354/394, to prevent TBK1 dimerization and activation. |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-276722 |
Tyr394 |
LNTIGLIyEKISLPK |
Homo sapiens |
HEK-293T Cell |
pmid |
sentence |
28618271 |
The Src family kinases (SFKs) Lck, Hck, and Fgr directly phosphorylate TBK1 at Tyr354/394, to prevent TBK1 dimerization and activation. |
|
Publications: |
2 |
Organism: |
Homo Sapiens |
+ |
ORF4b | down-regulates activity
binding
|
TBK1 |
0.2 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-260593 |
|
|
Homo sapiens |
HEK-293 Cell |
pmid |
sentence |
26631542 |
Previous studies have shown that MERS-CoV ORF4b antagonizes the early antiviral alpha/beta interferon (IFN-α/β) response, which may significantly contribute to MERS-CoV pathogenesis; however, the underlying mechanism is poorly understood. Here, we found that ORF4b in the cytoplasm could specifically bind to TANK binding kinase 1 (TBK1) and IκB kinase epsilon (IKKε), suppress the molecular interaction between mitochondrial antiviral signaling protein (MAVS) and IKKε, and inhibit IFN regulatory factor 3 (IRF3) phosphorylation and subsequent IFN-β production. these results indicate that MERS-CoV ORF4b inhibits the induction of type I IFN through a direct interaction with IKKε/TBK1 in the cytoplasm |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
Pathways: | COVID-19 Causal Network, SARS-CoV INNATE RESPONSE TO dsRNA |
+ |
DTX4 | down-regulates
ubiquitination
|
TBK1 |
0.567 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-71565 |
|
|
Homo sapiens |
Neuron |
pmid |
sentence |
10531053 |
Nlrp4 negatively regulates type i interferon signaling by targeting the kinase tbk1 for degradation via the ubiquitin ligase dtx4 |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
+ |
TBK1 | up-regulates activity
phosphorylation
|
NfKb-p65/p50 |
0.577 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-260157 |
|
|
Homo sapiens |
|
pmid |
sentence |
15489227 |
Constitutive and interleukin-1-inducible Phosphorylation of p65 NF-{kappa}B at Serine 536 Is Mediated by Multiple Protein Kinases Including I{kappa}B Kinase (IKK)-{alpha}, IKK{beta}, IKK{epsilon}, TRAF Family Member-Associated (TANK)-binding Kinase 1 (TBK1). Overexpressed ikkepsilon and tbk1 phosphorylate ser-536 in vivo and in vitro. |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
Pathways: | COVID-19 Causal Network, EBV infection, Innate Immune Response, Inflammosome Activation, SARS-CoV INFLAMMATORY RESPONSE, SARS-CoV INNATE RESPONSE TO dsRNA, Toll like receptors |
+ |
N-[3-[[5-Cyclopropyl-2-(4-morpholin-4-ylanilino)pyrimidin-4-yl]amino]propyl]cyclobutanecarboxamide | down-regulates activity
chemical activation
|
TBK1 |
0.8 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-261109 |
|
|
Mus musculus |
Primary Cell |
pmid |
sentence |
23099093 |
We herein describe the development of a novel series of 2,4-diamino-5-cyclopropyl pyrimidines as potent inhibitors of TBK1/IKKɛ, with improved kinase selectivity and drug-like properties. |
|
Publications: |
1 |
Organism: |
Mus Musculus |
+ |
N-[3-[[5-iodo-4-[3-[[oxo(thiophen-2-yl)methyl]amino]propylamino]-2-pyrimidinyl]amino]phenyl]-1-pyrrolidinecarboxamide | down-regulates
chemical inhibition
|
TBK1 |
0.8 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-190795 |
|
|
Homo sapiens |
|
pmid |
sentence |
Other |
|
|
Publications: |
1 |
Organism: |
Homo Sapiens |
+ |
MAVS | up-regulates activity
binding
|
TBK1 |
0.91 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-260145 |
|
|
Homo sapiens |
|
pmid |
sentence |
25636800 |
After ligand binding, cGAS and RIG-I signal through respective adaptor proteins STING and MAVS to recruit the kinases IKK and TBK1, which then activate the transcription factors NF-κB and interferon regulatory factor 3 (IRF3), respectively. |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
Pathways: | COVID-19 Causal Network, Innate Immune Response, SARS-CoV INNATE RESPONSE TO dsRNA |
+ |
PPM1B | down-regulates activity
dephosphorylation
|
TBK1 |
0.36 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-276985 |
|
|
in vitro |
|
pmid |
sentence |
22750291 |
PPM1B dephosphorylates TBK1 in vivo and in vitro.|These results demonstrate that PPM1B inhibits TBK1 mediated antiviral signaling by directly dephosphorylating TBK1 at Ser172. |
|
Publications: |
1 |
Organism: |
In Vitro |
+ |
OPTN | up-regulates activity
binding
|
TBK1 |
0.785 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-262276 |
|
|
Homo sapiens |
HEK-293 Cell |
pmid |
sentence |
20174559 |
Using biochemical experiments we showed that optineurin interacts with the protein kinase TBK1 and the ubiquitin ligase TRAF3. Furthermore, a mutation in optineurin that prevents the interaction with the small protein modifier ubiquitin (D474N) ablated the negative regulatory function of optineurin. |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
+ |
STING1 | up-regulates activity
binding
|
TBK1 |
0.2 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-260153 |
|
|
Homo sapiens |
|
pmid |
sentence |
24622840 |
MAVS also interacts with STING that locates at the ER (endoplasmic reticulum), and induces the ubiquitination and dimerization of STING. The activated STING recruits TBK1 and IRF3 and contributes to the phosphorylation of IRF3 mediated by TBK1. |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
Pathways: | COVID-19 Causal Network, Innate Immune Response, SARS-CoV INNATE RESPONSE TO dsRNA |
+ |
IKBKE | up-regulates activity
binding
|
TBK1 |
0.629 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-260155 |
|
|
Homo sapiens |
|
pmid |
sentence |
24622840 |
STING recruits TBK1 and IKKε and forms the TBK1-IKKε complex via the association with TRAF3. The TBK1 complex induces the phosphorylation, dimerization, and nuclear translocation of IRF3. |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
Pathways: | COVID-19 Causal Network, Innate Immune Response, SARS-CoV INNATE RESPONSE TO dsRNA, Toll like receptors |
+ |
TRIM23 | up-regulates activity
binding
|
TBK1 |
0.534 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-266654 |
|
|
Homo sapiens |
HEK-293 Cell |
pmid |
sentence |
28871090 |
TRIM23 interacts with TBK1 and p62. TRIM23 GTPase activates TBK1 to phosphorylate p62. Biochemical characterization showed that TRIM23 binds with its C-terminal ARF domain to the N-terminal KD of TBK1, and that GTP hydrolysis activity of the ARF stimulates TBK1-mediated phosphorylation of p62 at S403 in its ubiquitin-associated (UBA) domain, which was shown to promote cargo recruitment and autophagic flux |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
+ |
Helicase | down-regulates activity
binding
|
TBK1 |
0.2 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-262512 |
|
|
Homo sapiens |
HEK-293 Cell |
pmid |
sentence |
32979938 |
We use unbiased screening to identify SARS-CoV-2 proteins that antagonize type I interferon (IFN-I) response. We found three proteins that antagonize IFN-I production via distinct mechanisms: nonstructural protein 6 (nsp6) binds TANK binding kinase 1 (TBK1) to suppress interferon regulatory factor 3 (IRF3) phosphorylation, nsp13 binds and blocks TBK1 phosphorylation, and open reading frame 6 (ORF6) binds importin Karyopherin α 2 (KPNA2) to inhibit IRF3 nuclear translocation. the results indicate that (1) nsp6 binds to TBK1 without affecting TBK1 phosphorylation, but the nsp6/TBK1 interaction decreases IRF3 phosphorylation, which leads to reduced IFN-β production; and (2) nsp13 binds and inhibits TBK1 phosphorylation, resulting in decreased IRF3 activation and IFN-β production (Figure 2F). |
|
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-262511 |
|
|
Homo sapiens |
HEK-293 Cell |
pmid |
sentence |
32979938 |
We use unbiased screening to identify SARS-CoV-2 proteins that antagonize type I interferon (IFN-I) response. We found three proteins that antagonize IFN-I production via distinct mechanisms: nonstructural protein 6 (nsp6) binds TANK binding kinase 1 (TBK1) to suppress interferon regulatory factor 3 (IRF3) phosphorylation, nsp13 binds and blocks TBK1 phosphorylation, and open reading frame 6 (ORF6) binds importin Karyopherin α 2 (KPNA2) to inhibit IRF3 nuclear translocation. the results indicate that (1) nsp6 binds to TBK1 without affecting TBK1 phosphorylation, but the nsp6/TBK1 interaction decreases IRF3 phosphorylation, which leads to reduced IFN-β production; and (2) nsp13 binds and inhibits TBK1 phosphorylation, resulting in decreased IRF3 activation and IFN-β production (Figure 2F). |
|
Publications: |
2 |
Organism: |
Homo Sapiens |
Pathways: | SARS-CoV INFLAMMATORY RESPONSE, SARS-CoV INNATE RESPONSE TO dsRNA |
+ |
TICAM1 | up-regulates activity
binding
|
TBK1 |
0.814 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-118458 |
|
|
Homo sapiens |
HEK-293 Cell |
pmid |
sentence |
14530355 |
Toll/il-1 receptor domain-containing adaptor inducing ifn-beta (trif) associates with tnf receptor-associated factor 6 and tank-binding kinase 1, and activates two distinct transcription factors, nf-kappa b and ifn-regulatory factor-3, in the toll-like receptor signaling |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
Pathways: | COVID-19 Causal Network, EBV infection, Innate Immune Response, Inflammosome Activation, SARS-CoV INFLAMMATORY RESPONSE, SARS-CoV INNATE RESPONSE TO dsRNA, Toll like receptors |
+ |
MTURN | down-regulates activity
binding
|
TBK1 |
0.2 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-273664 |
|
|
Mus musculus |
MEF Cell |
pmid |
sentence |
28704656 |
Here we report that viral infection induced upregulation of INKIT, an inhibitor for NF-κB and IRF3 that restricted innate antiviral responses by blocking phosphorylation of p65 and IRF3. Viral infection induced IKK-mediated phosphorylation of INKIT at Ser58, resulting in its dissociation from the IKKs. INKIT is associated with IKKα/β and TBK1/IKKɛ and inhibits the recruitment and phosphorylation of p65 and IRF3, respectively. IKKα and TBK1 phosphorylate INKIT at Ser58, which results in disassociation of INKIT from IKKα or TBK1 and thereby allows for the subsequent recruitment and phosphorylation of p65 and IRF3 |
|
Publications: |
1 |
Organism: |
Mus Musculus |
+ |
TBKBP1 | up-regulates activity
relocalization
|
TBK1 |
0.623 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-272469 |
|
|
Homo sapiens |
|
pmid |
sentence |
31792381 |
TBKBP1 recruits TBK1 to protein kinase C-theta (PKCθ) through a scaffold protein, CARD10. This enables PKCθ to phosphorylate TBK1 at Ser 716, a crucial step for TBK1 activation |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
+ |
TBK1 | up-regulates
phosphorylation
|
REL/RELA |
0.583 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-217667 |
|
|
Homo sapiens |
|
pmid |
sentence |
16888014 |
The present results demonstrate that ikkepsilon- and tbk1-mediated phosphorylation of crel in the c-terminal td leads to cytoplasmic dissociation of a crel-ikb_ complex and nuclear accumulation of crel. |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
+ |
TRAF3 | up-regulates activity
binding
|
TBK1 |
0.9 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-260156 |
|
|
Homo sapiens |
|
pmid |
sentence |
24622840 |
MAVS also interacts with STING that locates at the ER (endoplasmic reticulum), and induces the ubiquitination and dimerization of STING. The activated STING recruits TBK1 and IRF3 and contributes to the phosphorylation of IRF3 mediated by TBK1. |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
Pathways: | COVID-19 Causal Network, Innate Immune Response, Inflammosome Activation, SARS-CoV INFLAMMATORY RESPONSE |
+ |
Non-structural protein 6 | down-regulates activity
binding
|
TBK1 |
0.2 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-262510 |
|
|
Homo sapiens |
|
pmid |
sentence |
32979938 |
We use unbiased screening to identify SARS-CoV-2 proteins that antagonize type I interferon (IFN-I) response. We found three proteins that antagonize IFN-I production via distinct mechanisms: nonstructural protein 6 (nsp6) binds TANK binding kinase 1 (TBK1) to suppress interferon regulatory factor 3 (IRF3) phosphorylation, nsp13 binds and blocks TBK1 phosphorylation, and open reading frame 6 (ORF6) binds importin Karyopherin α 2 (KPNA2) to inhibit IRF3 nuclear translocation. the results indicate that (1) nsp6 binds to TBK1 without affecting TBK1 phosphorylation, but the nsp6/TBK1 interaction decreases IRF3 phosphorylation, which leads to reduced IFN-β production; and (2) nsp13 binds and inhibits TBK1 phosphorylation, resulting in decreased IRF3 activation and IFN-β production (Figure 2F). |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
Pathways: | SARS-CoV INFLAMMATORY RESPONSE, SARS-CoV INNATE RESPONSE TO dsRNA |
+ |
TBK1 | up-regulates
phosphorylation
|
REL |
0.565 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-148623 |
|
|
Homo sapiens |
|
pmid |
sentence |
16888014 |
The present results demonstrate that ikkepsilon- and tbk1-mediated phosphorylation of crel in the c-terminal td leads to cytoplasmic dissociation of a crel-ikb_ complex and nuclear accumulation of crel. |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
+ |
TFEB | up-regulates quantity by expression
transcriptional regulation
|
TBK1 |
0.293 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-276802 |
|
|
|
|
pmid |
sentence |
30145926 |
Inhibition of DNM or dynein-mediated endocytic trafficking for up to 1 h resulted in translocation of TFEB-GFP to the nucleus in P8B11-HeLa cells (Figure 5(a-c) and a correlated increase in transcription of TFEB-target genes, including MAP1LC3/LC3, SQSTM1, MCOLN1, CTSB, CTSF, and TFEB |
|
Publications: |
1 |
+ |
TBK1 | up-regulates activity
binding
|
IKBKE |
0.629 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-178053 |
|
|
Homo sapiens |
|
pmid |
sentence |
18353649 |
Whereas nemo assembles some but not all ikk complexes [12,13], recent reports provide strong experimental evidence for a role of tank [also called traf-interacting protein (i-traf)], nak-associated protein (nap1) and similar to nap1 tbk1 adaptor (sintbad) in the assembly of tbk1 and ikk-e kinase complexes that phosphorylate irf3 and irf7 and promote type i ifn gene induction |
|
Publications: |
1 |
Organism: |
Homo Sapiens |
Pathways: | COVID-19 Causal Network, Innate Immune Response, SARS-CoV INNATE RESPONSE TO dsRNA, Toll like receptors |
+ |
TBK1 | up-regulates
binding
|
IKBKB |
0.412 |
Identifier |
Residue |
Sequence |
Organism |
Cell Line |
SIGNOR-121576 |
|
|
Homo sapiens |
|
pmid |
sentence |
14743216 |
A physical and functional map of the human tnf-alpha/nf-kappa b signal transduction pathway. |
|
Publications: |
1 |
Organism: |
Homo Sapiens |